Univariate analysis of prognostic variables in overall survival (OS) for patients.

*<p>Significant difference.</p

Kaplan-Meier curves for patient survival.

<p>(A) Patients with an erbB2-positive tumor showed a significantly shorter survival period after surgery than those with an erbB2-negative tumor (p  = 0.014). (B) Patients with a flotillin2-positive tumor showed the worst outcome. High flotillin-2 and erbB2 expression are significantly correlated with poor patient outcome (p<0.001).</p

Flotillin2 expression in relation to the status of erbB2 expression.

<p>Figures in parentheses are percentages, % is within ErbB2</p>*<p>p<0.05 considered to be statistically significant.</p

Multivariate analysis of overall survival after surgery.

<p>HR hazard radio, CI confidence interval, *Significant difference.</p

Correlation of Flotillin2 and erbB2 expression with clinicopathologic parameters in gastric cancer.

<p>P values are based on a X2 test, *Significant difference</p

Knockdown of flotillin-2 resulted in a stronger reduction of erbB2.

<p>Amplification of HER2 gene was determined by FISH. HER2 gene amplification in a cluster pattern (red) was observed in (A) MGC-803, (B) BGC-823 and (C) SGC-7901 gastric cancer cells, HER2 over expression in human SGC-7901 cell. (D) Western blot analysis and (E) Quantitative real-time PCR were accessed for erbB2 and flotillin2 in SGC-7901 cell line after knockdown of flotillin-2. The expression status of flotillin2 is highly concordant with that of erbB2, siRNA was blank vector, β-Actin was used as internal control.</p

Correlation between LDH-A expression and prognosis of patients with GC.

<p>Kaplan-Meier curves for cumulative survival rate of patients with GC according to GC tissue LDH-A expression(a), Kaplan-Meier curves for cumulative survival rate of patients with intestinal-type GC(b) and diffuse-type GC(c) stratified by the Lauren grade, and Kaplan-Meier curves for differentiated type GC(d) and undifferentiated type GC(e) stratified according to Histological type.</p

Immunohistochemical staining of LDH-A in gastric samples.

<p>LDH-A was expressed in the cytoplasm of NNM and carcinomas: <b>a</b> LDH-A positivity was observed in the cytoplasm of NNM, <b>b</b> LDH-A was detected in the cytoplasm of differentiated gastric carcinoma, <b>c</b> and in the poorly-differentiated gastric carcinoma.</p

Relationship between LDH-A expression and clinico-pathologic features of GC.

<p>Relationship between LDH-A expression and clinico-pathologic features of GC.</p

Univariate and multivariate analysis for overall survival after surgery using cox RR relative risk.

<p>Univariate and multivariate analysis for overall survival after surgery using cox RR relative risk.</p