Cell migration of ADSCs as determined by wound scratch assays.

<p>(A) Quantification of the “wound gap” distance between the front lines of migrating cells. (B) Images taken at 0, 6, 12, 24, and 48 hours to visualize the wound gap (n = 3, * p<0.05).</p

Adipogenic differentiation of ADSCs.

<p>(A) Adipose differentiation of ADSCs evaluated by Oil red O staining. (B) Quantitative analysis of the intracellular Oil red O by spectrophotometry (n = 3, * p<0.05).</p

A Comparative Study on the Biological Characteristics of Human Adipose-Derived Stem Cells from Lipectomy and Liposuction - Fig 13

<p><b>The LDH levels of ADSCs treated with different concertations of lidocaine (A) and adrenaline (B)</b> (n = 3, * p<0.05).</p

Cell attachment rate of ADSCs from lipectomy and liposuction (n = 3, * p<0.05).

<p>Cell attachment rate of ADSCs from lipectomy and liposuction (n = 3, * p<0.05).</p

The proliferation of ADSCs from lipectomy and liposuction detected by CCK-8 assay (n = 4, * p<0.05).

<p>The proliferation of ADSCs from lipectomy and liposuction detected by CCK-8 assay (n = 4, * p<0.05).</p

The paracrine secretion of ADSCs measured by ELISA.

<p>VEGF (A), IL-6 (B), IL-8 (C), and HGF (D) expression, when ADSCs were treated with different concertations of insulin and TGF-α(n = 3, p>0.05).</p

Cell viability of ADSCs from lipectomy and liposuction detected by Trypan blue staining (n = 3, * p<0.05).

<p>Cell viability of ADSCs from lipectomy and liposuction detected by Trypan blue staining (n = 3, * p<0.05).</p

The proliferation of ADSCs, treated with different concertation of lidocaine and adrenaline, detected by CCK-8 assays.

<p>The proliferation of ADSCs, treated with different concertation of lidocaine and adrenaline, detected by CCK-8 assays.</p

Oxidative stress assessment of ADSCs by detecting the SOD and MDA level.

<p>The SOD and MDA levels were detected by Xanthine oxidase and thiobarbituric acid methods, respectively (n = 3, * p<0.05).</p

The apoptosis of ADSCs treated with lidocaine or adrenaline.

<p>ADSCs were treated with different concentrations of lidocaine (A) or adrenaline (C) for 2 hours, and then the apoptosis of cell was measured using flow cytometry analysis after staining with Annexin V-FITC/PI. (B), (D) Quantification of apoptosis in the cells treated with different concentrations of lidocaine or adrenaline, respectively (n = 3, * p<0.05).</p