44 research outputs found
Hydrogen-Bonded Multilayers of Silk Fibroin: From Coatings to Cell-Mimicking Shaped Microcontainers
We present a novel type of all-aqueous nonionic layer-by-layer
films of silk fibroin with synthetic macromolecules and a natural
polyphenol. We found the multilayer growth and stability to be strongly
pH-dependent. Silk assembled with poly(methacrylic) and tannic acids
at pH = 3.5 disintegrated at pH ∼ 5, while silk/poly(<i>N</i>-vinylcaprolactam) interactions were stable at low and
high pH values but resulted in thinner films at a high pH. The results
suggest that the intermolecular interactions are primarily driven
by hydrogen bonding with a considerable contribution of hydrophobic
forces. We also demonstrated that cubical, spherical, and platelet
capsules with silk-containing walls can be constructed using particulate
sacrificial templates. This work sets a foundation for future explorations
of natural and synthetic macromolecules assemblies as biomimetic materials
with tunable properties
Additional file 9: of In-depth comparative analysis of malaria parasite genomes reveals protein-coding genes linked to human disease in Plasmodium falciparum genome
Table S5. Candidate genes related to virulence of the P. falciparum parasite. (XLSX 31Â kb
Additional file 6: of In-depth comparative analysis of malaria parasite genomes reveals protein-coding genes linked to human disease in Plasmodium falciparum genome
Figure S2. Genomic location of 115 P. falciparum genes. (TIF 968Â kb
Additional file 4: of In-depth comparative analysis of malaria parasite genomes reveals protein-coding genes linked to human disease in Plasmodium falciparum genome
Table S3. The candidate P. falciparum genes probably responsible for parasitizing human erythrocytes. (XLSX 16Â kb
Additional file 2: of In-depth comparative analysis of malaria parasite genomes reveals protein-coding genes linked to human disease in Plasmodium falciparum genome
Figure S1. Clusters composed of members from a single species or six species. a) Clusters comprise P. vavix genes (left panel) or P. falciparum genes (right panel). b) Clusters comprising genes from six Plasmodium species. (TIF 1617Â kb
Additional file 8: of In-depth comparative analysis of malaria parasite genomes reveals protein-coding genes linked to human disease in Plasmodium falciparum genome
Figure S4. Expression dynamics of SURF family members in the intraerythrocytic cycle of the P. falciparum parasite. (TIF 75Â kb
In Situ Reductive Synthesis of Structural Supported Gold Nanorods in Porous Silicon Particles for Multifunctional Nanovectors
Porous silicon nanodisks
(PSD) were fabricated by the combination of photolithography and electrochemical
etching of silicon. By using PSD as a reducing agent, gold nanorods
(AuNR) were in situ synthesized in the nanopores of PSD, forming PSD-supported-AuNR
(PSD/AuNR) hybrid particles. The formation mechanism of AuNR in porous
silicon (pSi) was revealed by exploring the role of pSi reducibility
and each chemical in the reaction. With the PSD support, AuNR exhibited
a stable morphology without toxic surface ligands (CTAB). The PSD/AuNR
hybrid particles showed enhanced plasmonic property compared to free
AuNR. Because high-density “hot spots” can be generated
by controlling the distribution of AuNR supported in PSD, surface-enhanced
raman scattering (SERS) using PSD/AuNR as particle substrates was
demonstrated. A multifunctional vector, PSD/AuNR/DOX, composed of
doxorubicin (DOX)-loaded PSD/AuNR capped with agarose (agar), was
developed for highly efficient, combinatorial cancer treatment. Their
therapeutic efficacy was examined using two pancreatic cancer cell
lines, PANC-1 and MIA PaCa-2. PSD/AuNR/DOX (20 μg Au and 1.25
μg DOX/mL) effectively destroyed these cells under near-IR laser
irradiation (810 nm, 15 J·cm<sup>–2</sup> power, 90 s).
Overall, we envision that PSD/AuNR may be a promising injectable,
multifunctional nanovector for biomedical application
Additional file 5: of In-depth comparative analysis of malaria parasite genomes reveals protein-coding genes linked to human disease in Plasmodium falciparum genome
Table S4. The P. berghei genes included in rodent malaria parasite-enriched clusters. (XLSX 20Â kb
Additional file 7: of In-depth comparative analysis of malaria parasite genomes reveals protein-coding genes linked to human disease in Plasmodium falciparum genome
Figure S3. Genomic location of 267 P. berghei genes. (TIF 2034Â kb
Additional file 11: of In-depth comparative analysis of malaria parasite genomes reveals protein-coding genes linked to human disease in Plasmodium falciparum genome
Table S6. Identified P. falciparum genes that possibly contribute to cerebral malaria. (XLSX 12Â kb