MOESM1 of Heterologous expression of Spathaspora passalidarum xylose reductase and xylitol dehydrogenase genes improved xylose fermentation ability of Aureobasidium pullulans

Additional file 1: Table S1. Primers used for genetic manipulation of A. pullulans var. melanogenum CBS 110374

MOESM2 of Heterologous expression of Spathaspora passalidarum xylose reductase and xylitol dehydrogenase genes improved xylose fermentation ability of Aureobasidium pullulans

Additional file 2: Figure S1. The sketch map for knock-in of XI gene by one-step homologous recombination-based method. Figure S2. PCR verification of the transformant with overexpressed XI gene. Figure S3. PCR verification of the transformant with overexpressed XYL1.1 (A), XYL1.2 (B), XYL2.1 (C) and XYL2.2 (D). Figure S4. PCR verification of the transformant with overexpressed XYL1.1 and XYL2.1 (A), XYL1.1 and XYL2.2 (B), XYL1.2 and XYL2.1 (C), XYL1.2 and XYL2.2 (D). Figure S5. Comparing the production of pullulan, heavy oil and melanin of the strains with single overexpressed XR gene or XDH gene with that of the parent strain