QTL analysis of L<i>er/</i>Cvi RIL population for primary root length under 40 mM CaCl₂ condition.

<p>LOD score values from multiple-QTL model mapping (MQM) analysis of traits plotted against a linear representation of the <i>Arabidopsis</i> genome. Red lines represent LOD score values. Dashed lines indicated the threshold value. Four QTLs were identified in chrom.1, 2, and 5 and named response to high calcium (<i>RHCA</i>) 1–4.</p

The response of L<i>er</i>/Cvi near-isogenic lines (NILs) containing QTLs on chromosome 1 and 5 to high calcium.

<p>(A) Analysis of primary root length of L<i>er</i>/Cvi NIL lines grown on different medium. Data presented as means ± SD (n≥5). The different lowercase letters on the bars indicate significantly different means (P<0.05). (B) The graphic genotypes of NIL lines. White column indicates L<i>er</i> genome, and black column indicates Cvi introgression fragment.</p

Frequency distribution of primary root length of L<i>er</i>/Cvi RIL population grown under 40 mM CaCl₂ condition.

<p>Arrows indicate the levels in the parental lines: L<i>er</i> and Cvi.</p

The primary root length of L<i>er</i> and Cvi seedlings on different medium with high CaCl_2, NaCl and KCl.

<p>(A) Pictures of L<i>er</i> and Cvi seedlings grown on different medium (Scale bar = 1 cm). (B) Primary root length analysis of L<i>er</i> and Cvi seedlings grown under different conditions. Average values ± SD are shown (n = 5). The different lowercase letters on the bars indicate significantly different means (P<0.05).</p

Natural variation of high Ca response in <i>Arabidopsis</i>.

<p>(A–C) Pictures of different <i>Arabidopsis</i> accessions grown on medium with or without 40 and 50 mM CaCl₂ (Scale bar = 1 cm). Shakdara (Sha, N929), Cape Verde Islands (Cvi, N902), Landsberg <i>erecta</i> (L<i>er,</i> N1642), An-1(N99), Columbia (Col-0, N1092), Eri. (D) Analysis of primary root length of <i>Arabidopsis</i> accessions under different calcium conditions. Data presented as means± SD (n≥3). The different lowercase letters on the bars indicate significantly different means (P<0.05).</p