Design of double-notch UWB filter with upper stopband characteristics based on ACPW-DGS.

In this manuscript, a compact (size only 9.8mm*9.8mm) Ultra Wide Band (UWB) bandpass filter with a new structure is proposed, which can be used in the UWB wireless communication band authorized by the FCC. The top plane is composed of a pair of back-to-back microstrip lines, and the ground plane structure is based on an asymmetric coplanar waveguide-defect ground structure (ACPW-DGS). UWB is formed by the vertical electromagnetic coupling of the top plane and the ground plane. On this basis, split ring resonator (SRR) and C type resonator (CTR) are utilized to place double notch bands. A novel third order nested C-type resonator (TONCTR) is obtained by performing CTR, which can further optimize the upper stopband while ensuring double notch bands. The filter can be used for filtering within the UWB system, and it can also avoid the amateur radio band (9.2 -10.3GHz) and the X-band satellite link band (9.6-12.3GHz) on UWB communication systems. Finally, the measured results from the fabricated prototype are basically consistent with the simulation results

Risk and prognosis of secondary lung cancer after radiation therapy for thoracic malignancies

Abstract Objective Radiation therapy (RT) may increase the risk of second cancer. This study aimed to determine the association between exposure to radiotherapy for the treatment of thoracic cancer (TC) and subsequent secondary lung cancer (SLC). Materials and Methods The Surveillance, Epidemiology, and End Results (SEER) database (from 1975 to 2015) was queried for TC. Univariate Cox regression analyses and multiple primary standardized incidence ratios (SIRs) were used to assess the risk of SLC. Subgroup analyses of patients stratified by latency time since TC diagnosis, age at TC diagnosis, and calendar year of TC diagnosis stage were also performed. Overall survival and SLC‐related death were compared among the RT and no radiation therapy (NRT) groups by using Kaplan–Meier analysis and competitive risk analysis. Results In a total of 329 129 observations, 147 847 of whom had been treated with RT. And 6799 patients developed SLC. Receiving radiotherapy was related to a higher risk of developing SLC for TC patients (adjusted HR, 1.25; 95% CI, 1.19–1.32; P < 0.001). The cumulative incidence of developing SLC in TC patients with RT (3.8%) was higher than the cumulative incidence (2.9%) in TC patients with NRT(P). The incidence risk of SLC in TC patients who received radiotherapy was significantly higher than the US general population (SIR, 1.19; 95% CI, 1.14–1.23; P < 0.050). Conclusions Radiotherapy for TC was associated with higher risks of developing SLC compared with patients unexposed to radiotherapy

Correction to: Construction of cell factory capable of efficiently converting l‑tryptophan into 5‑hydroxytryptamine

BACKGROUND: l-Tryptophan (l-Trp) derivatives such as 5-hydroxytryptophan (5-HTP) and 5-hydroxytryptamine (5-HT), N-Acetyl-5-hydroxytryptamine and melatonin are important molecules with pharmaceutical interest. Among, 5-HT is an inhibitory neurotransmitter with proven benefits for treating the symptoms of depression. At present, 5-HT depends on plant extraction and chemical synthesis, which limits its mass production and causes environmental problems. Therefore, it is necessary to develop an efficient, green and sustainable biosynthesis method to produce 5-HT. RESULTS: Here we propose a one-pot production of 5-HT from l-Trp via two enzyme cascades for the first time. First, a chassis cell that can convert l-Trp into 5-HTP was constructed by heterologous expression of tryptophan hydroxylase from Schistosoma mansoni (SmTPH) and an artificial endogenous tetrahydrobiopterin (BH(4)) module. Then, dopa decarboxylase from Harminia axyridis (HaDDC), which can specifically catalyse 5-HTP to 5-HT, was used for 5-HT production. The cell factory, E. coli BL21(DE3)△tnaA/BH(4)/HaDDC-SmTPH, which contains SmTPH and HaDDC, was constructed for 5-HT synthesis. The highest concentration of 5-HT reached 414.5 ± 1.6 mg/L (with conversion rate of 25.9 mol%) at the optimal conditions (substrate concentration,2 g/L; induced temperature, 25℃; IPTG concentration, 0.5 mM; catalysis temperature, 30℃; catalysis time, 72 h). CONCLUSIONS: This protocol provided an efficient one-pot method for converting. l-Trp into 5-HT production, which opens up possibilities for the practical biosynthesis of natural 5-HT at an industrial scale. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12934-022-01745-0

The Spatial Structure and Driving Mechanisms of Multi-Source Networks in the Chengdu–Chongqing Economic Circle of China

The phenomenon of polarized development among regional cities has sparked extensive contemplation and indicated a need for research on multi-source regional networks. However, such research faces two obstacles: the absence of quantitative measurement of differences in network structures and the lack of a thorough examination of the degree of city clustering and the dynamics of community composition in hierarchical networks. Thus, we identified 16 cities in the Chengdu–Chongqing Economic Circle (CCEC) as the spatial units to examine the spatial network structures of population, resources, and transportation and the integrated spatial network structure. Using social network analysis, this paper describes the structural characteristics of the three networks (population, resource, and transportation), followed by an analysis of their collective and hierarchical network clustering characteristics, and explores the driving mechanisms and factors that make up each network model. Our results show the following: (1) All three networks exhibit an “east dense, west sparse” characteristic, but there are differences in the layouts of the core cities in terms of the three networks. (2) The clustering characteristics of the hierarchical networks are more pronounced than those of the overall network. The results of the analysis combined with the network formation mechanisms can help effectively plan the future coordinated development of the CCEC

Weak-Texture Seafloor and Land Image Matching Using Homography-Based Motion Statistics with Epipolar Geometry

The matching of remote sensing images is a critical and necessary procedure that directly impacts the correctness and accuracy of underwater topography, change detection, digital elevation model (DEM) generation, and object detection. The texture of images becomes weaker with increasing water depth, and this results in matching-extraction failure. To address this issue, a novel method, homography-based motion statistics with an epipolar constraint (HMSEC), is proposed to improve the number, reliability, and robustness of matching points for weak-textured seafloor images. In the matching process of HMSEC, a large number of reliable matching points can be identified from the preliminary matching points based on the motion smoothness assumption and motion statistics. Homography and epipolar geometry are also used to estimate the scale and rotation influences of each matching point in image pairs. The results show that the matching-point numbers for the seafloor and land regions can be significantly improved. In this study, we evaluated this method for the areas of Zhaoshu Island, Ganquan Island, and Lingyang Reef and compared the results to those of the grid-based motion statistics (GMS) method. The increment of matching points reached 2672, 2767, and 1346, respectively. In addition, the seafloor matching points had a wider distribution and reached greater water depths of −11.66, −14.06, and −9.61 m. These results indicate that the proposed method could significantly improve the number and reliability of matching points for seafloor images

ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance

The ATR-Chk1 pathway is essential in cellular responses to DNA damage and replication stress, whereas the role of long noncoding RNAs (lncRNAs) in regulating this pathway remains largely unknown. In this study, we identify an ATR and Chk1 interacting lncRNA (ACIL, also known as LRRC75A-AS1 or SNHG29), which promotes the phosphorylation of Chk1 by ATR upon DNA damages. High ACIL levels are associated with chemoresistance to DNA damaging agents and poor outcome of breast cancer patients. ACIL knockdown sensitizes breast cancer cells to DNA damaging drugs in vitro and in vivo. ACIL protects cancer cells against DNA damages by inducing cell cycle arrest, stabilizing replication forks and inhibiting unscheduled origin firing, thereby guarding against replication catastrophe and contributing to DNA damage repair. These findings demonstrate a lncRNA-dependent mechanism of activating the ATR-Chk1 pathway and highlight the potential of utilizing ACIL as a predictive biomarker for chemotherapy sensitivity, as well as targeting ACIL to reverse chemoresistance in breast cancer

Activation of Bivalent Gene POU4F1 Promotes and Maintains Basal‐like Breast Cancer

Abstract Basal‐like breast cancer (BLBC) is the most aggressive molecular subtype of breast cancer with worse prognosis and fewer treatment options. The underlying mechanisms upon BLBC transcriptional dysregulation and its upstream transcription factors (TFs) remain unclear. Here, among the hyperactive candidate TFs of BLBC identified by bioinformatic analysis, POU4F1 is uniquely upregulated in BLBC and is associated with poor prognosis. POU4F1 is necessary for the tumor growth and malignant phenotypes of BLBC through regulating G1/S transition by direct binding at the promoter of CDK2 and CCND1. More importantly, POU4F1 maintains BLBC identity by repressing ERα expression through CDK2‐mediated EZH2 phosphorylation and subsequent H3K27me3 modification in ESR1 promoter. Knocking out POU4F1 in BLBC cells reactivates functional ERα expression, rendering BLBC sensitive to tamoxifen treatment. In‐depth epigenetic analysis reveals that the subtype‐specific re‐configuration and activation of the bivalent chromatin in the POU4F1 promoter contributes to its unique expression in BLBC, which is maintained by DNA demethylase TET1. Together, these results reveal a subtype‐specific epigenetically activated TF with critical role in promoting and maintaining BLBC, suggesting that POU4F1 is a potential therapeutic target for BLBC