(S)-tert-Butyl 3-(3-phenyl-1,2,4-oxa­diazol-5-yl)piperidine-1-carboxyl­ate

The title compound, C18H23N3O3, crystallized with two independent mol­ecules (A and B) in the asymmetric unit. The phenyl ring and the 1,2,4-oxadiazole ring are inclined to one another by 19.9 (3)° in mol­ecule A and 7.3 (3)° in mol­ecule B. The absolute structure of the title compound was referred to the transfered chiral center (S) of one of the starting reacta­nts. In the crystal, A mol­ecules are linked by C—H⋯N inter­actions involving the two oxadiazole N atoms

Point Cloud Upsampling via Cascaded Refinement Network

Point cloud upsampling focuses on generating a dense, uniform and proximity-to-surface point set. Most previous approaches accomplish these objectives by carefully designing a single-stage network, which makes it still challenging to generate a high-fidelity point distribution. Instead, upsampling point cloud in a coarse-to-fine manner is a decent solution. However, existing coarse-to-fine upsampling methods require extra training strategies, which are complicated and time-consuming during the training. In this paper, we propose a simple yet effective cascaded refinement network, consisting of three generation stages that have the same network architecture but achieve different objectives. Specifically, the first two upsampling stages generate the dense but coarse points progressively, while the last refinement stage further adjust the coarse points to a better position. To mitigate the learning conflicts between multiple stages and decrease the difficulty of regressing new points, we encourage each stage to predict the point offsets with respect to the input shape. In this manner, the proposed cascaded refinement network can be easily optimized without extra learning strategies. Moreover, we design a transformer-based feature extraction module to learn the informative global and local shape context. In inference phase, we can dynamically adjust the model efficiency and effectiveness, depending on the available computational resources. Extensive experiments on both synthetic and real-scanned datasets demonstrate that the proposed approach outperforms the existing state-of-the-art methods.Comment: The first two authors contributed equally to this work. The code is publicly available at https://github.com/hikvision-research/3DVision. Accepted to ACCV 2022 as oral presentatio

Glomerular Organization of the Antennal Lobes of the Diamondback Moth, Plutella xylostella L.

The antennal lobe of the moth brain is the primary olfactory center processing information concerning pheromones and plant odors. Plutella xylostella is a major worldwide pest of cruciferous vegetables and its behavior is highly dependent on their olfactory system. However, detailed knowledge of the anatomy and function of the P. xylostella olfactory system remains limited. In the present study, we present the 3-Dimentional (3-D) map of the antennal lobe of P. xylostella, based on confocal microscopic analysis of glomerular segmentation and Neurobiotin backfills of Olfactory Receptor Neurons (ORNs). We identified 74–76 ordinary glomeruli and a macroglomerular complex (MGC) situated at the entrance of the antennal nerve in males. The MGC contained three glomeruli. The volumes of glomeruli in males ranged from 305.83 ± 129.53 to 25440.00 ± 1377.67 μm3. In females, 74–77 glomeruli were found, with the largest glomerulus ELG being situated at the entrance of the antennal nerve. The volumes of glomeruli in females ranged from 802.17 ± 95.68 to 8142.17 ± 509.46 μm3. Sexual dimorphism was observed in anomalous supernumerary, anomalous missing, shape, size, and array of several of the identified glomeruli in both sexes. All glomeruli, except one in the antennal lobe (AL), received projections of antennal ORNs. The glomeruli PV1 in both sexes received input from the labial palp nerve and was assumed as the labial pit organ glomerulus (LPOG). These results provide a foundation for better understanding of coding mechanisms of odors in this important pest insect

Energy-Based Controller Design of Stochastic Magnetic Levitation System

This paper investigates the control problem of magnetic levitation system, in which velocity feedback signal is influenced by stochastic disturbance. Firstly, single-degree-freedom magnetic levitation is regarded as an energy-transform action device. From the view of energy-balance relation, the magnetic levitation system is transformed into port-controlled Hamiltonian system model. Next, based on the Hamiltonian structure, the control law of magnetic levitation system is designed by applying Lyapunov theory. Finally, the simulation verifies the correctness of the proposed results

MicroRNA-141 Represses HBV Replication by Targeting PPARA

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression primarily at the post-transcriptional level and play critical roles in a variety of physiological and pathological processes. In this report, miR-141 was identified to repress HBV expression by screening a small miRNA expressing library and synthetic miR-141 mimics could also significantly suppress HBV expression and replication in HepG2 cells. Bioinformatic analysis and experiment assays indicate that peroxisome proliferator-activated receptor alpha (PPARA) was the target of hsa-miR-141 during this process. Furthermore, knockdown of PPARA by small interfering RNA (siRNA) inhibited HBV replication similar to levels observed for miR-141. Promoter functional analysis indicated that repression of HBV replication by miR-141 mimics or siRNA was mediated by interfering with the HBV promoter functions, consistent with previous studies demonstrating that PPARA regulated HBV gene expression through interactions with HBV promoter regulatory elements. Our results suggest that miR-141 suppressed HBV replication by reducing HBV promoter activities by down-regulating PPARA. This study provides new insights into the molecular mechanisms associated with HBV-host interactions. Furthermore, this information may facilitate the development of novel anti-HBV therapeutic strategies

FBNet: Feedback Network for Point Cloud Completion

The rapid development of point cloud learning has driven point cloud completion into a new era. However, the information flows of most existing completion methods are solely feedforward, and high-level information is rarely reused to improve low-level feature learning. To this end, we propose a novel Feedback Network (FBNet) for point cloud completion, in which present features are efficiently refined by rerouting subsequent fine-grained ones. Firstly, partial inputs are fed to a Hierarchical Graph-based Network (HGNet) to generate coarse shapes. Then, we cascade several Feedback-Aware Completion (FBAC) Blocks and unfold them across time recurrently. Feedback connections between two adjacent time steps exploit fine-grained features to improve present shape generations. The main challenge of building feedback connections is the dimension mismatching between present and subsequent features. To address this, the elaborately designed point Cross Transformer exploits efficient information from feedback features via cross attention strategy and then refines present features with the enhanced feedback features. Quantitative and qualitative experiments on several datasets demonstrate the superiority of proposed FBNet compared to state-of-the-art methods on point completion task.Comment: The first two authors contributed equally to this work. The source code and model are available at https://github.com/hikvision-research/3DVision/. Accepted to ECCV 2022 as oral presentatio

Application of Polypyrrole (Ppy) nanofibers in Solid Phase Extraction of samples before the determination of vitamin B families in infant powder

In this study, a kind of core/sheath Polystyrene/Polypyrrole (PS/PPy) electrospun nanofibers was prepared and used as the solid phase extraction (SPE) adsorbent. Primary extraction of target analytes, three kinds of vitamin B (VB2, riboflavin; VB9, folic acid; VB12, cobalamin), was carried out by loading samples onto the column along with appointed boric acid compound reagent, and then the column should also be rinsed with the same reagent solution before elution. The boric acid compound was applied as complexing reagent to retain as much of three analytes as possible on the column based on the multi interaction between three vitamins and the boronate affinity reagent, thus improving hydrophobicity of targets and adsorption efficiency through loading and rinsing steps. Analytical parameters include linearity r2> 0.99; limit of detection (LOD) ranged from 0.05 to 0.093μg/ml; Intraday and Interday precision of the method, 0.59%-12.1% and 0.64%-14.5%, respectively; the spiked extraction recoveries ranged from 84.9% to 111.7% in the infant powder samples. A Ppy nanofibers SPE column coupled with HPLC-UV was established for assay of elements in infant powder. The levels of three vitamin Bs in three brands of real milk powder samples were determined and the results were compared with the prescription. Results showed that the analysis method in the paper were reliable to be used in the determination of vitamin B families in infant powder

Geniposide Alleviates Glucocorticoid-Induced Inhibition of Osteogenic Differentiation in MC3T3-E1 Cells by ERK Pathway

Glucocorticoid (GC) therapy is the leading cause of secondary osteoporosis and the therapeutic and preventative drugs for GC-induced osteoporosis are limited. In this study, we investigated the protective effects of geniposide on dexamethasone (DEX)-induced osteogenic inhibition in MC3T3-E1 cells. The results showed that there was no obvious toxicity on MC3T3-E1 cells when geniposide was used at the doses ranging from 1 to 75 μM. In DEX-treated MC3T3-E1 cells, geniposide promoted the alkaline phosphatase (ALP) activity and the mineralization. In addition, geniposide also significantly increased the mRNA and protein expression of osteopontin (OPN), Runt-related transcription factor 2 (Runx2), and Osterix (Osx) in DEX-treated MC3T3-E1 cells. Furthermore, geniposide activated ERK pathway in DEX-treated MC3T3-E1 cells. The ERK activation inhibitor U0126 and glucagon-like peptide-1 (GLP-1) receptor antagonist exendin 9-39 abolished the geniposide-induced activation of ERK and inhibited the protective effect of geniposide. Taken together, our study revealed that geniposide alleviated GC-induced osteogenic suppression in MC3T3-E1 cells. The effect of geniposide was at least partially associated with activating ERK signaling pathway via GLP-1 receptor. Geniposide might be a potential therapeutic agent for GC-induced osteoporosis