1 research outputs found
Construction of Nanodroplet/Adiposome and Artificial Lipid Droplets
The lipid droplet (LD) is a cellular
organelle that consists of
a neutral lipid core with a monolayer-phospholipid membrane and associated
proteins. Recent LD studies demonstrate its importance in metabolic
diseases and biofuel development. However, the mechanisms governing
its formation and dynamics remain elusive. Therefore, we developed
an <i>in vitro</i> system to facilitate the elucidation
of these mechanisms. We generated sphere-shaped structures with a
neutral lipid core and a monolayer-phospholipid membrane by mechanically
mixing neutral lipids and phospholipids followed by a two-step purification.
We named the nanodroplet “adiposome”. We then recruited
LD structure-like/resident proteins to the adiposome, including the
bacterial MLDS, Caenorhabditis elegans MDT-28/PLIN-1, or mammalian perilipin-2. In addition, adipose triglyceride
lipase (ATGL) and apolipoprotein A1 (apo A-I) were recruited to adiposome.
We termed the functional protein-coated adiposomes, Artificial Lipid
Droplets (ALDs). With this experimental system, different proteins
can be recruited to build ALDs for some biological goals and potential
usage in drug delivery