10 research outputs found
DataSheet_3_Regulation and bioinformatic analysis of circ_0015891/miR-129-1-3p axis in methamphetamine-induced dopaminergic apoptosis.zip
Methamphetamine (METH) abuse can result in severe neurotoxicity, for which the mechanism is not yet clear. In the present study, we investigated the role of noncoding RNAs in METH-induced dopaminergic neurotoxicity, and analyzed the underlying mechanism using bioinformatic methods. We confirmed by flow cytometry that miR-129-1-3p is involved in promoting dopaminergic apoptosis under METH treatment and its role could be inhibited by a high concentration of circ_0015891. Also, we combined transcriptomic data with bioinformatics to explore the downstream mechanism of miR-129-1-3p regulation of METH-induced apoptosis, highlighted the potentially pivotal figure of response to nutrition. Further bioinformatic analysis of circ_0015891 was conducted as well and showed that circ_0015891 was the sponge of various microRNAs that effect apoptosis by different mechanisms. Collectively, we found a novel circ_0015891/miR-129-1-3p axis that may be a promising therapeutic target for METH-induced dopaminergic neurotoxicity.</p
DataSheet_1_Regulation and bioinformatic analysis of circ_0015891/miR-129-1-3p axis in methamphetamine-induced dopaminergic apoptosis.zip
Methamphetamine (METH) abuse can result in severe neurotoxicity, for which the mechanism is not yet clear. In the present study, we investigated the role of noncoding RNAs in METH-induced dopaminergic neurotoxicity, and analyzed the underlying mechanism using bioinformatic methods. We confirmed by flow cytometry that miR-129-1-3p is involved in promoting dopaminergic apoptosis under METH treatment and its role could be inhibited by a high concentration of circ_0015891. Also, we combined transcriptomic data with bioinformatics to explore the downstream mechanism of miR-129-1-3p regulation of METH-induced apoptosis, highlighted the potentially pivotal figure of response to nutrition. Further bioinformatic analysis of circ_0015891 was conducted as well and showed that circ_0015891 was the sponge of various microRNAs that effect apoptosis by different mechanisms. Collectively, we found a novel circ_0015891/miR-129-1-3p axis that may be a promising therapeutic target for METH-induced dopaminergic neurotoxicity.</p
DataSheet_2_Regulation and bioinformatic analysis of circ_0015891/miR-129-1-3p axis in methamphetamine-induced dopaminergic apoptosis.zip
Methamphetamine (METH) abuse can result in severe neurotoxicity, for which the mechanism is not yet clear. In the present study, we investigated the role of noncoding RNAs in METH-induced dopaminergic neurotoxicity, and analyzed the underlying mechanism using bioinformatic methods. We confirmed by flow cytometry that miR-129-1-3p is involved in promoting dopaminergic apoptosis under METH treatment and its role could be inhibited by a high concentration of circ_0015891. Also, we combined transcriptomic data with bioinformatics to explore the downstream mechanism of miR-129-1-3p regulation of METH-induced apoptosis, highlighted the potentially pivotal figure of response to nutrition. Further bioinformatic analysis of circ_0015891 was conducted as well and showed that circ_0015891 was the sponge of various microRNAs that effect apoptosis by different mechanisms. Collectively, we found a novel circ_0015891/miR-129-1-3p axis that may be a promising therapeutic target for METH-induced dopaminergic neurotoxicity.</p
Quantitative Analyses of Dynamic Features of Fibroblasts on Different Protein-Coated Compliant Substrates
Cell
response to substrate rigidity, closely related to extracellular
matrix protein composition, requires actomyosin-generated contractility.
By introducing coefficients describing cell spreading and traction
dynamics, and a revised high-resolution traction force microscopy,
we analyzed the static and dynamic features of fibroblasts on fibronectin-
or collagen- coated stiff or soft substrates. Large cell spreading
area and branchlike morphology were more favorable on fibronectin
than collagen. Cell spreading on fibronectin-coated substrates was
more sensitive to rigidity compared with collagen. Low concentration
fibronectin-coated substrate induced more dynamic lamellipodia movement
than other conditions. Interestingly, the static average cell traction
on high concentration fibronectin-coated stiff and soft substrates
showed no difference. However, the lamellipodium traction dynamics
was sensitive to rigidity on fibronectin. Particularly, lamellipodia
on fibronectin-coated soft substrate performed much higher local traction
dynamics compared with other groups. Together, dynamics of cell adhesion
and traction are regulated by extracellular matrix protein composition,
coupled with substrate rigidity
Quantitative Analyses of Dynamic Features of Fibroblasts on Different Protein-Coated Compliant Substrates
Cell
response to substrate rigidity, closely related to extracellular
matrix protein composition, requires actomyosin-generated contractility.
By introducing coefficients describing cell spreading and traction
dynamics, and a revised high-resolution traction force microscopy,
we analyzed the static and dynamic features of fibroblasts on fibronectin-
or collagen- coated stiff or soft substrates. Large cell spreading
area and branchlike morphology were more favorable on fibronectin
than collagen. Cell spreading on fibronectin-coated substrates was
more sensitive to rigidity compared with collagen. Low concentration
fibronectin-coated substrate induced more dynamic lamellipodia movement
than other conditions. Interestingly, the static average cell traction
on high concentration fibronectin-coated stiff and soft substrates
showed no difference. However, the lamellipodium traction dynamics
was sensitive to rigidity on fibronectin. Particularly, lamellipodia
on fibronectin-coated soft substrate performed much higher local traction
dynamics compared with other groups. Together, dynamics of cell adhesion
and traction are regulated by extracellular matrix protein composition,
coupled with substrate rigidity
Quantitative Analyses of Dynamic Features of Fibroblasts on Different Protein-Coated Compliant Substrates
Cell
response to substrate rigidity, closely related to extracellular
matrix protein composition, requires actomyosin-generated contractility.
By introducing coefficients describing cell spreading and traction
dynamics, and a revised high-resolution traction force microscopy,
we analyzed the static and dynamic features of fibroblasts on fibronectin-
or collagen- coated stiff or soft substrates. Large cell spreading
area and branchlike morphology were more favorable on fibronectin
than collagen. Cell spreading on fibronectin-coated substrates was
more sensitive to rigidity compared with collagen. Low concentration
fibronectin-coated substrate induced more dynamic lamellipodia movement
than other conditions. Interestingly, the static average cell traction
on high concentration fibronectin-coated stiff and soft substrates
showed no difference. However, the lamellipodium traction dynamics
was sensitive to rigidity on fibronectin. Particularly, lamellipodia
on fibronectin-coated soft substrate performed much higher local traction
dynamics compared with other groups. Together, dynamics of cell adhesion
and traction are regulated by extracellular matrix protein composition,
coupled with substrate rigidity
Significance of Epitaxial Growth of PtO<sub>2</sub> on Rutile TiO<sub>2</sub> for Pt/TiO<sub>2</sub> Catalysts
TiO2-supported Pt species have been widely
applied in
numerous critical reactions involving photo-, thermo-, and electrochemical-catalysis
for decades. Manipulation of the state of the Pt species in Pt/TiO2 catalysts is crucial for fine-tuning their catalytic performance.
Here, we report an interesting discovery showing the epitaxial growth
of PtO2 atomic layers on rutile TiO2, potentially
allowing control of the states of active Pt species in Pt/TiO2 catalysts. The presence of PtO2 atomic layers
could modulate the geometric configuration and electronic state of
the Pt species under reduction conditions, resulting in a spread of
the particle shape and obtaining a Pt/PtO2/TiO2 structure with more positive valence of Pt species. As a result,
such a catalyst exhibits exceptional electrocatalytic activity and
stability toward hydrogen evolution reaction, while also promoting
the thermocatalytic CO oxidation, surpassing the performance of the
Pt/TiO2 catalyst with no epitaxial structure. This novel
epitaxial growth of the PtO2 structure on rutile TiO2 in Pt/TiO2 catalysts shows its potential in the
rational design of highly active and economical catalysts toward diverse
catalytic reactions
Revealing the Size Effect of Ceria Nanocube-Supported Platinum Nanoparticles in Complete Propane Oxidation
The
elimination of propane is one of the key tasks in
reducing
volatile organic compounds (VOCs) and automotive exhaust emissions.
The platinum nanoparticle (NP) is a promising catalyst for propane
oxidation, while the study of its structural characteristics and functionality
remains in its infancy. In this work, we synthesized the nanocubes
CeO2 with a well-defined (100) facet supporting Pt NPs
with various sizes, from 1.3 to 7 nm, and systematically investigated
the effect of the Pt size on complete propane oxidation efficiency.
In particular, CeO2(100) supported Pt NPs smaller than
4 nm promote the formation of positively charged Pt sites, which hinder
the adsorption and activation of propane and reduce the intrinsic
activity for propane oxidation. Consequently, within this size range,
the catalytic performance is primarily influenced by the electronic
state of the Pt species, with metallic Pt being identified as the
active site for the reaction. Conversely, as the particle size exceeds
4 nm, metallic Pt particles become dominant and the geometric structure
starts to influence the activity as well. Such entanglement of electronic
and geometric factors gives rise to a volcano relationship between
reaction rates and Pt particle sizes ranging from 1.3 to 7 nm, while
an increased correlation can be observed between the turnover frequencies
and the particle sizes in this range. This knowledge can guide the
synthesis of highly active catalysts, enabling the efficient oxidation
of VOCs with reduced precious metal loadings
Additional file 1 of NOD1 deficiency ameliorates the progression of diabetic retinopathy by modulating bone marrow–retina crosstalk
Additional file 1. Supplementary data
Atomically Dispersed Dual Metal Sites Boost the Efficiency of Olefins Epoxidation in Tandem with CO<sub>2</sub> Cycloaddition
Tandem catalysis provides an economical and energy-efficient
process
for the production of fine chemicals. In this work, we demonstrate
that a rationally synthesized carbon-based catalyst with atomically
dispersed dual Fe–Al sites (ADD-Fe-Al) achieves superior catalytic
activity for the one-pot oxidative carboxylation of olefins (conversion
∼97%, selectivity ∼91%), where the yield of target product
over ADD-Fe-Al is at least 62% higher than that of monometallic counterparts.
The kinetic results reveal that the excellent catalytic performance
arises from the synergistic effect between Fe (oxidation site) and
Al sites (cycloaddition site), where the efficient CO2 cycloaddition
with epoxides in the presence of Al sites (3.91 wt %) positively shifts
the oxidation equilibrium to olefin epoxidation over Fe sites (0.89
wt %). This work not only offers an advanced catalyst for oxidative
carboxylation of olefins but also opens up an avenue for the rational
design of multifunctional catalysts for tandem catalytic reactions
in the future