Effect of miR-200c on nasopharyngeal carcinoma and the probable molecular regulatory mechanism involved

Purpose: To determine the effect of micro-ribonucleic acid-200c (miR-200c) on biological function of nasopharyngeal carcinoma, and the likely molecular regulatory mechanism involved.Methods: Thirty (30) nasopharyngeal carcinoma tissues and para-cancerous normal tissues were taken from patients undergoing surgery in Maternal and Child Health Hospital of Hubei Province, Wuhan from September 2018 to January 2020. The expression levels of miR-200c in the two types of tissues were determined. Cells of human nasopharyngeal carcinoma cell line HNE1 were cultured to about 70 % growth prior to transfection with blank plasmid, PINI and miR-200c analogs. After 48 h of culture, control group, PINI group, and miR-200c over-expression + PINI group were obtained. The expression levels of PINI and changes in centrosomes in each group were measured, and changes in cell migration in each group were determined using Transwell migration assay.Results: Compared with para-cancerous normal tissues, the expression level of miR-200c in nasopharyngeal carcinoma was  significantly increased (p < 0.01). Compared with the control group, the PINI expression level and cell migration ability in miR-200c overexpression tissue were markedly decreased, while the percentage of extra centrosomes was markedly increased. Compared to miR-200c over-expression tissue, the PINI expression level and cell migration ability in the miR-200c overexpression + PINI group were markedly raised, while the percentage of extra-central somatic cells was significantly decreased (p < 0.05).Conclusion: MiR-200c may inhibit the migration of nasopharyngeal carcinoma cells by inhibiting the expression of PINI and inhibiting abnormal expansion of centrosomes. Keywords: MiR-200c, Nasopharyngeal carcinoma, Biological function, Molecular regulatory mechanis

OR-022 Effects of aerobic exercise on the hemodynamics and structure of the common carotid artery in obese adolescents

Objective With the population of obese adolescents increases dramatically, a series of cardiovascular diseases, especially atherosclerotic, are triggered by obese which seriously threatens the life and health of teenagers. The aim of this study is to investigate the effects of aerobic exercise intervention on the hemodynamics and structure of the common carotid artery in obese adolescents. Methods  Forty obese adolescents (18 ± 2years) were randomly assigned into the experimental group (EG; n = 20) and control group (CG; n = 20). EG undertook 12 weeks of aerobic exercise training (AET), CG had not any exercise intervention. The carotid artery of both CG and EG were examined and compared. Carotid artery responses were assessed in both groups. Color doppler ultrasound was used to determine the tube diameter and axial flow of the common carotid before and after exercise intervention. The heart rate, systolic and diastolic blood pressure were simultaneously measured on the left brachial artery by a sphygmomanometer. Results Compared with CG, there were improvements of EG in peripheral resistance (22.90±6.70 VS 29.58±8.71. p＜0.01) and Systolic blood pressure (123.57±7.36 VS 130.25±6.79. p＜0.05) were verified after AET, except diastolic blood pressure. Following AET, blood flow velocity (0.28±0.05 VS 0.21±0.05. p＜0.01) and wall shear stress (6.25±0.90 VS 4.97±1.54. p＜0.05) increased prominently, which were also significant differences only in EG. In contrast, the vascular diameter demonstrated consistently upper compared with CG, but no differences between EG and CG. Conclusions  Regular aerobic exercise lasting 12 weeks could effectively change the dynamic parameters of the common carotid artery in obese adolescents, but no changes in arterial diameter. These findings indicated that 12 weeks of aerobic exercise can induce some changes of the common carotid artery blood flow within the circulation function in a short time. But the changing in common carotid arteries structure is needed after a long-term blood flow to the stimulation

Implications of C1q/TNF-related protein superfamily in patients with coronary artery disease.

The C1q complement/TNF-related protein superfamily (CTRPs) displays differential effects on the regulation of metabolic homeostasis, governing cardiovascular function. However, whether and how they may serve as predictor/pro-diagnosis factors for assessing the risks of coronary artery disease (CAD) remains controversial. Therefore, we performed a clinical study to elaborate on the implication of CTRPs (CTRP1, CTRP5, CTRP7, and CTRP15) in CAD. CTRP1 were significantly increased, whereas CTRP7 and CTRP15 levels were decreased in CAD patients compared to the non-CAD group. Significant differences in CTRP1 levels were discovered between the single- and triple-vascular-vessel lesion groups. ROC analysis revealed that CTRP7 and CTRP15 may serve as CAD markers, while CTRP1 may serve as a marker for the single-vessel lesion of CAD. CTRP1 and CTRP5 can serve as markers for the triple-vessel lesion. CTRP1 may serve as an independent risk predictor for triple-vessel lesion, whereas CTRP15 alteration may serve for a single-vessel lesion of CAD. CTRP1 may serve as a novel superior biomarker for diagnosis of severity of vessel-lesion of CAD patients. CTRP7, CTRP15 may serve as more suitable biomarker for the diagnosis of CAD patients, whereas CTRP5 may serve as an independent predictor for CAD. These findings suggest CTRPs may be the superior predictive factors for the vascular lesion of CAD and represent novel therapeutic targets against CAD

The Role of Tumour Metabolism in Cisplatin Resistance

Cisplatin is a chemotherapy drug commonly used in cancer treatment. Tumour cells are more sensitive to cisplatin than normal cells. Cisplatin exerts an antitumour effect by interfering with DNA replication and transcription processes. However, the drug-resistance properties of tumour cells often cause loss of cisplatin efficacy and failure of chemotherapy, leading to tumour progression. Owing to the large amounts of energy and compounds required by tumour cells, metabolic reprogramming plays an important part in the occurrence and development of tumours. The interplay between DNA damage repair and metabolism also has an effect on cisplatin resistance; the molecular changes to glucose metabolism, amino acid metabolism, lipid metabolism, and other metabolic pathways affect the cisplatin resistance of tumour cells. Here, we review the mechanism of action of cisplatin, the mechanism of resistance to cisplatin, the role of metabolic remodelling in tumorigenesis and development, and the effects of common metabolic pathways on cisplatin resistance

A comparative study of prepulse inhibition in children with first episode schizophrenia and normal children

Objective·To explore the characteristics of sensory gating and its variation in children with first episode schizophrenia (COS) by using a new technique of prepulse suppression (PI).Methods·By using the ERP recording and analysis system of brain products, PI was detected in 56 patients with COS and 38 healthy children (NC) using the paradigm of single strong stimulus and weak stimulus+strong stimulus. The patients′ performance was comprehensively evaluated with Positive and Negative Syndrome Scale (PANSS), Social Disability Screening Schedule (SDSS), Social Adjustment Rating Scale (SSRS), and Family Interview Schedule (FIS).Results·The social objective support formed by summing up the above scales was compared with the quantitative stanard of social support [the standard of social support scale was (8±2) points, and the value of COS group was (10±3) points], and the difference was statistically significant (P=0.007). No correlation was found between PI and PANSS total score and each factor score (all P>0.05). The latency of startle reflex in the COS group was longer than that in the NC group [the NC group was (86±11) ms, the COS group was (97±13) ms, P=0.001]. In the COS group, the amplitude of startle reflex of weak stimulus+strong stimulus was higher, and the latency was longer than that of the NC group [NC group: (39±12) μV, COS group (47±21) μV, P=0.007; the latency of the normal group was (84±17) ms, and that of the COS group was (97±20) ms, P=0.003]. PI inhibition rate in the COS group was lower than that in the NC group [(66±32) % in the NC group, (43±37) % in the COS group, P=0.000].Conclusion·COS patients have the same PI abnormality as adult schizophrenia. The change of PI inhibition may be the result of biological markers reflecting the change of agitated emotion in COS patients

A clinical evaluation of amlexanox oral adhesive pellicles in the treatment of recurrent aphthous stomatitis and comparison with amlexanox oral tablets: a randomized, placebo controlled, blinded, multicenter clinical trial

<p>Abstract</p> <p>Background</p> <p>Amlexanox has been developed as a 5 percent topical oral paste for the treatment of patients with recurrent aphthous stomatitis (RAS) in most European countries. However, it is not yet available in China and has not been generally accepted in clinical treatment. The aim of this study was to explore the effectiveness of amlexanox oral adhesive pellicles in the treatment of minor recurrent aphthous ulcers, and compare the results with those of amlexanox oral adhesive tablets in order to analyse the difference between the two dosage forms of amlexanox.</p> <p>Methods</p> <p>We performed a randomized, blinded, placebo-controlled, parallel, multicenter clinical study. A total of 216 patients with minor recurrent aphthous ulcers (MiRAU) were recruited and randomized to amlexanox pellicles or placebo pellicles. Pellicles were consecutively applied four times per day, for five days. The size and pain level of ulcers were measured and recorded on treatment days 0, 4 and 6. Finally, the results were compared with those of our previous 104 cases treated with amlexanox tablets.</p> <p>Results</p> <p>Amlexanox oral adhesive pellicles significantly reduced ulcer size (P= 0.017 for day 4, P=0.038 for day 6) and alleviated ulcer pain (P=0.021 for day 4, P=0.036 for day 6). No significant difference was observed in the treatment effectiveness between the pellicle and tablet form of amlexanox.</p> <p>Conclusions</p> <p>Amlexanox oral adhesive pellicles are as effective and safe as amlexanox oral adhesive tablets in the treatment of MiRAU for this Chinese cohort. However, pellicles seem to be more comfortable to use when compared with the dosage form of tablets. Therefore, in clinical practice, amlexanox oral adhesive pellicles may be a better choice for RAS patients.</p> <p>Trials registration</p> <p>Nederlands Trial Register NTR1727.</p