Flow-based assembly of nucleic acid-loaded polymer nanoparticles

Since the development of messenger RNA (mRNA)-based SARS-CoV-2 (COVID-19) vaccines, there is increased public awareness of the importance of nanoparticles, in this case lipid nanoparticles, to ensure safe delivery of an active compound. To ensure the formation of high-quality nanoparticles with reproducible results, these lipid nanoparticles are assembled with the nucleic acid drug using flow-based devices. Although flow assembly using lipid nanoparticles for nucleic acid delivery is well described in the literature, only a few examples use polymers. This is surprising because the field of polymers for nucleic acid delivery is substantial as hundreds of polymers for nucleic acid delivery have been reported in the literature. In this review, we discuss several aspects of flow-based assembly of nucleic acid-loaded polymer nanoparticles. Initially, we introduce the concept of chip-based or capillary-based systems that can be either used as single-phase or multiphase systems. Initially, researchers have to choose the type of mixing, which can be active or passive. The type of flow, laminar or turbulent, also significantly affects the quality of the nanoparticles. We then present the type of polymers that have so far been assembled with mRNA, small interfering RNA (siRNA) or plasmid DNA (pDNA) using flow devices. We discuss effects such as flow rate, concentration and polymer lengths on the outcome. To conclude, we highlight how flow assembly is an excellent way to generate well-defined nanoparticles including polyplexes in a reproducible manner

Surface molecular pump enables ultrahigh catalyst activity.

The performance of electrocatalysts is critical for renewable energy technologies. While the electrocatalytic activity can be modulated through structural and compositional engineering following the Sabatier principle, the insufficiently explored catalyst-electrolyte interface is promising to promote microkinetic processes such as physisorption and desorption. By combining experimental designs and molecular dynamics simulations with explicit solvent in high accuracy, we demonstrated that dimethylformamide can work as an effective surface molecular pump to facilitate the entrapment of oxygen and outflux of water. Dimethylformamide disrupts the interfacial network of hydrogen bonds, leading to enhanced activity of the oxygen reduction reaction by a factor of 2 to 3. This strategy works generally for platinum-alloy catalysts, and we introduce an optimal model PtCuNi catalyst with an unprecedented specific activity of 21.8 ± 2.1 mA/cm2 at 0.9 V versus the reversible hydrogen electrode, nearly double the previous record, and an ultrahigh mass activity of 10.7 ± 1.1 A/mgPt

Unsupervised Anomaly Detection via Variational Auto-Encoder for Seasonal KPIs in Web Applications

To ensure undisrupted business, large Internet companies need to closely monitor various KPIs (e.g., Page Views, number of online users, and number of orders) of its Web applications, to accurately detect anomalies and trigger timely troubleshooting/mitigation. However, anomaly detection for these seasonal KPIs with various patterns and data quality has been a great challenge, especially without labels. In this paper, we proposed Donut, an unsupervised anomaly detection algorithm based on VAE. Thanks to a few of our key techniques, Donut greatly outperforms a state-of-arts supervised ensemble approach and a baseline VAE approach, and its best F-scores range from 0.75 to 0.9 for the studied KPIs from a top global Internet company. We come up with a novel KDE interpretation of reconstruction for Donut, making it the first VAE-based anomaly detection algorithm with solid theoretical explanation.Comment: 12 pages (including references), 17 figures, submitted to WWW 2018: The 2018 Web Conference, April 23--27, 2018, Lyon, France. The contents discarded from the conference version due to the 9-page limitation are also included in this versio

CKD-TransBTS: Clinical Knowledge-Driven Hybrid Transformer with Modality-Correlated Cross-Attention for Brain Tumor Segmentation

Brain tumor segmentation (BTS) in magnetic resonance image (MRI) is crucial for brain tumor diagnosis, cancer management and research purposes. With the great success of the ten-year BraTS challenges as well as the advances of CNN and Transformer algorithms, a lot of outstanding BTS models have been proposed to tackle the difficulties of BTS in different technical aspects. However, existing studies hardly consider how to fuse the multi-modality images in a reasonable manner. In this paper, we leverage the clinical knowledge of how radiologists diagnose brain tumors from multiple MRI modalities and propose a clinical knowledge-driven brain tumor segmentation model, called CKD-TransBTS. Instead of directly concatenating all the modalities, we re-organize the input modalities by separating them into two groups according to the imaging principle of MRI. A dual-branch hybrid encoder with the proposed modality-correlated cross-attention block (MCCA) is designed to extract the multi-modality image features. The proposed model inherits the strengths from both Transformer and CNN with the local feature representation ability for precise lesion boundaries and long-range feature extraction for 3D volumetric images. To bridge the gap between Transformer and CNN features, we propose a Trans&CNN Feature Calibration block (TCFC) in the decoder. We compare the proposed model with five CNN-based models and six transformer-based models on the BraTS 2021 challenge dataset. Extensive experiments demonstrate that the proposed model achieves state-of-the-art brain tumor segmentation performance compared with all the competitors

HoVer-Trans: Anatomy-aware HoVer-Transformer for ROI-free Breast Cancer Diagnosis in Ultrasound Images

Ultrasonography is an important routine examination for breast cancer diagnosis, due to its non-invasive, radiation-free and low-cost properties. However, the diagnostic accuracy of breast cancer is still limited due to its inherent limitations. It would be a tremendous success if we can precisely diagnose breast cancer by breast ultrasound images (BUS). Many learning-based computer-aided diagnostic methods have been proposed to achieve breast cancer diagnosis/lesion classification. However, most of them require a pre-define ROI and then classify the lesion inside the ROI. Conventional classification backbones, such as VGG16 and ResNet50, can achieve promising classification results with no ROI requirement. But these models lack interpretability, thus restricting their use in clinical practice. In this study, we propose a novel ROI-free model for breast cancer diagnosis in ultrasound images with interpretable feature representations. We leverage the anatomical prior knowledge that malignant and benign tumors have different spatial relationships between different tissue layers, and propose a HoVer-Transformer to formulate this prior knowledge. The proposed HoVer-Trans block extracts the inter- and intra-layer spatial information horizontally and vertically. We conduct and release an open dataset GDPH&SYSUCC for breast cancer diagnosis in BUS. The proposed model is evaluated in three datasets by comparing with four CNN-based models and two vision transformer models via five-fold cross validation. It achieves state-of-the-art classification performance with the best model interpretability. In the meanwhile, our proposed model outperforms two senior sonographers on the breast cancer diagnosis when only one BUS image is given

Active IRS-Aided MIMO Systems: How Much Gain Can We Get?

Intelligent reflecting surfaces (IRSs) have emerged as a promising technology to improve the efficiency of wireless communication systems. However, passive IRSs suffer from the ``multiplicative fading" effect, because the transmit signal will go through two fading hops. With the ability to amplify and reflect signals, active IRSs offer a potential way to tackle this issue, where the amplification energy only experiences the second hop. However, the fundamental limit and system design for active IRSs have not been fully understood, especially for multiple-input multiple-output (MIMO) systems. In this work, we consider the analysis and design for the large-scale active IRS-aided MIMO system assuming only statistical channel state information (CSI) at the transmitter and the IRS. The evaluation of the fundamental limit, i.e., ergodic rate, turns out to be a very difficult problem. To this end, we leverage random matrix theory (RMT) to derive the deterministic approximation (DA) for the ergodic rate, and then design an algorithm to jointly optimize the transmit covariance matrix at the transmitter and the reflection matrix at the active IRS. Numerical results demonstrate the accuracy of the derived DA and the effectiveness of the proposed optimization algorithm. The results in this work reveal interesting physical insights with respect to the advantage of active IRSs over their passive counterparts