Global fitness profiling of fission yeast deletion strains by barcode sequencing

A genome-wide deletion library is a powerful tool for probing gene functions and one has recently become available for the fission yeast Schizosaccharomyces pombe. Here we use deep sequencing to accurately characterize the barcode sequences in the deletion library, thus enabling the quantitative measurement of the fitness of fission yeast deletion strains by barcode sequencing

Coherence-protected Quantum Gate by Continuous Dynamical Decoupling in Diamond

To implement reliable quantum information processing, quantum gates have to be protected together with the qubits from decoherence. Here we demonstrate experimentally on nitrogen-vacancy system that by using continuous wave dynamical decoupling method, not only the coherence time is prolonged by about 20 times, but also the quantum gates is protected for the duration of controlling time. This protocol shares the merits of retaining the superiority of prolonging the coherence time and at the same time easily combining with quantum logic tasks. It is expected to be useful in task where duration of quantum controlling exceeds far beyond the dephasing time.Comment: 5 pages, 4 figure

Two types of generalized integrable decompositions and new solitary-wave solutions for the modified Kadomtsev-Petviashvili equation with symbolic computation

The modified Kadomtsev-Petviashvili (mKP) equation is shown in this paper to be decomposable into the first two soliton equations of the 2N-coupled Chen-Lee-Liu and Kaup-Newell hierarchies by respectively nonlinearizing two sets of symmetry Lax pairs. In these two cases, the decomposed (1+1)-dimensional nonlinear systems both have a couple of different Lax representations, which means that there are two linear systems associated with the mKP equation under the same constraint between the potential and eigenfunctions. For each Lax representation of the decomposed (1+1)-dimensional nonlinear systems, the corresponding Darboux transformation is further constructed such that a series of explicit solutions of the mKP equation can be recursively generated with the assistance of symbolic computation. In illustration, four new families of solitary-wave solutions are presented and the relevant stability is analyzed.Comment: 23 page

Quantum Discord for Investigating Quantum Correlations without Entanglement in Solids

Quantum systems unfold diversified correlations which have no classical counterparts. These quantum correlations have various different facets. Quantum entanglement, as the most well known measure of quantum correlations, plays essential roles in quantum information processing. However, it has recently been pointed out that quantum entanglement cannot describe all the nonclassicality in the correlations. Thus the study of quantum correlations in separable states attracts widely attentions. Herein, we experimentally investigate the quantum correlations of separable thermal states in terms of quantum discord. The sudden change of quantum discord is observed, which captures ambiguously the critical point associated with the behavior of Hamiltonian. Our results display the potential applications of quantum correlations in studying the fundamental properties of quantum system, such as quantum criticality of non-zero temperature.Comment: 4 pages, 4 figure

Association of Mitochondrial DNA Polymerase γ Gene <i>POLG1</i> Polymorphisms with Parkinsonism in Chinese Populations

Background: Mitochondrial DNA polymerase gamma (POLG1) mutations were associated with levodopa-responsive Parkinsonism. POLG1 gene contains a number of common nonsynonymous SNPs and intronic regulatory SNPs which may have functional consequences. It is of great interest to discover polymorphisms variants associated with Parkinson's disease (PD), both in isolation and in combination with specific SNPs.Materials and Methods: We conducted a case-control study and genotyped twenty SNPs and poly-Q polymorphisms of POLG1 gene including in 344 Chinese sporadic PD patients and 154 healthy controls. All the polymorphisms of POLG1 we found in this study were sequenced by PCR products with dye terminator methods using an ABI-3100 sequencer. Hardy-Weinberg equilibrium and linkage disequilibrium (LD) for association between twenty POLG1 SNPs and PD were calculated using the program Haploview.Principal Results: We provided evidence for strong association of four intronic SNPs of the POLG1 gene (new report: c.2070-12T>A and rs2307439: c.2070-64G>A in intron 11, P = 0.00011, OR = 1.727; rs2302084: c.3105-11T>C and rs2246900: c.3105-36A>G in intron 19, P = 0.00031, OR = 1.648) with PD. However, we did not identify any significant association between ten exonic SNPs of POLG1 and PD. Linkage disequilibrium analysis indicated that c.2070-12T>A and c.2070-64G>A could be parsed into one block as Haplotype 1 as well as c.3105-11T>C and c.3105-36A>G in Haplotype 2. In addition, case and control study on association of POLG1 CAG repeat (poly-Q) alleles with PD showed a significant association (P = 0.03, OR = 2.16) of the non-10/11Q variants with PD. Although intronic SNPs associated with PD didn't influence POLG1 mRNA alternative splicing, there was a strong association of c.2070-12T>A and c.2070-64G>A with decreased POLG1 mRNA level and protein levels.Conclusions: Our findings indicate that POLG1 may play a role in the pathogenesis of PD in Chinese populations.</p

Research on Chloride Penetration Resistance of Phosphoaluminate Cement Concrete

The chloride penetration resistance of phosphoaluminate cement (PAC) concrete was investigated in this study. The effects of mixture proportioning parameters, including cement content, water–cement ratio (w/c), and superplasticizer content on chloride ion penetration resistance of PAC concrete were investigated by the rapid chloride migration test (RCM). The result shows that the increase of cement content and the decrease of w/c can reduce the coefficient of chloride ion penetration of PAC concrete at a certain range. Moreover, the penetration of chloride ion can be declined effectively when polycarboxylate superplasticizer is employed. The PAC concrete exhibited better chloride penetration resistance compared with normal Portland cement (PC) concrete prepared with the same mixture proportioning parameters. This is due to the fact that the hydration mechanism and the hydration products of PAC concrete are different from that of PC concrete

The role of EGFR mutation as a prognostic factor in survival after diagnosis of brain metastasis in non-small cell lung cancer: A systematic review and meta-analysis

Abstract Background The brain is a common site for metastasis in non-small-cell lung cancer (NSCLC). This study was designed to evaluate the relationship between the mutational of the epidermal growth factor receptor (EGFR) and overall survival (OS) in NSCLC patients with brain metastases. Methods Searches were performed in PubMed, EmBase, and the Cochrane Library to identify studies evaluating the association of EGFR mutation with OS in NSCLC patients through September 2017. Results 4373 NSCLC patients with brain metastases in 18 studies were involved. Mutated EGFR associated with significantly improved OS compared with wild type. Subgroup analyses suggested that this relationship persisted in studies conducted in Eastern, with retrospective design, with sample size ≥500, mean age of patients ≥65.0 years, percentage male < 50.0%, percentage of patients receiving tyrosine kinase inhibitor ≥30.0%. Finally, although significant publication bias was observed using the Egger test, the results were not changed after adjustment using the trim and fill method. Conclusions This meta-analysis suggests that EGFR mutation is an important predictive factor linked to improved OS for NSCLC patients with brain metastases. It can serve as a useful index in the prognostic assessment of NSCLC patients with brain metastases

Distribution amplitudes of light diquarks

Accumulating evidence indicates that soft quark+quark (diquark) correlations play an important role in the structure and interactions of hadrons constituted from three or more valence-quarks; so, it is worth developing insights into diquark structure. Using a leading-order truncation of those equations needed to solve continuum two-valence-body bound-state problems, the leading-twist two-parton distribution amplitudes (DAs) of light-quark scalar and pseudovector diquarks are calculated. The diquark DAs are narrower and taller than the asymptotic profile that characterises mesons. Consequently, the valence quasiparticles in a diquark are less likely to carry a large light-front fraction of the system's total momentum than those in a meson. These features may both influence the form of baryon DAs and be transmitted to diquark distribution functions (DFs), in which case their impact will be felt, e.g. in the proton's $u$ and $d$ valence-quark DFs.Comment: 8 pages, 2 figures, 1 table. Accepted for publication in Eur. Phys. J. A (Lett

Iterative Learning Control of a Nonlinear Aeroelastic System despite Gust Load

The development of a control strategy appropriate for the suppression of aeroelastic vibration of a two-dimensional nonlinear wing section based on iterative learning control (ILC) theory is described. Structural stiffness in pitch degree of freedom is represented by nonlinear polynomials. The uncontrolled aeroelastic model exhibits limit cycle oscillations beyond a critical value of the free-stream velocity. Using a single trailing-edge control surface as the control input, a ILC law under alignment condition is developed to ensure convergence of state tracking error. A novel Barrier Lyapunov Function (BLF) is incorporated in the proposed Barrier Composite Energy Function (BCEF) approach. Numerical simulation results clearly demonstrate the effectiveness of the control strategy toward suppressing aeroelastic vibration in the presence of parameter uncertainties and triangular, sinusoidal, and graded gust loads