Dynamic Model and Fault Feature Research of Dual-Rotor System with Bearing Pedestal Looseness

The paper presents a finite element model of dual-rotor system with pedestal looseness stemming from loosened bolts. Dynamic model including bearing pedestal looseness is established based on the dual-rotor test rig. Three-degree-of-freedom (DOF) planar rigid motion of loose bearing pedestal is fully considered and collision recovery coefficient is also introduced in the model. Based on the Timoshenko beam elements, using the finite element method, rigid body kinematics, and the Newmark-β algorithm for numerical simulation, dynamic characteristics of the inner and outer rotors and the bearing pedestal plane rigid body motion under bearing pedestal looseness condition are studied. Meanwhile, the looseness experiments under two different speed combinations are carried out, and the experimental results are basically the same. The simulation results are compared with the experimental results, indicating that vibration displacement waveforms of loosened rotor have “clipping” phenomenon. When the bearing pedestal looseness fault occurs, the inner and outer rotors vibration spectrum not only contains the difference and sum frequency of the two rotors’ fundamental frequency but also contains 2X and 3X component of rotor with loosened support, and so forth; low frequency spectrum is more, containing dividing component, and so forth; the rotor displacement spectrums also contain fewer combination frequency components, and so forth; when one side of the inner rotor bearing pedestal is loosened, the inner rotor axis trajectory is drawn into similar-ellipse shape

Rapid and reliable differential display from minute amounts of tissue: mass cloning and characterization of differentially expressed genes from loblolly pine embryos

"December 1997.""Submitted to Plant Molecular Biology Reporter.

Transcript profiling: a tool to assess the development of conifer embryos

"March 2000.""Submitted to In Vitro Cellular and Developmental Biology-Plant.

Loblolly pine somatic embryogenesis: development of a maturation medium and resulting embryo quality

"May 2002."Submitted to Plant Cell Reports

Differential display: a tool to follow natural and somatic embryo development in loblolly pine

"July 1997.""Submitted to Tappi R and D Division Biological Sciences Symposium, San Francisco, California, October 20-23, 1997.

A novel mesenchymal stem cell-based regimen for acute myeloid leukemia differentiation therapy

Currently the main treatment of acute myeloid leukemia (AML) is chemotherapy combining hematopoietic stem cell transplantation. However, the unbearable side effect of chemotherapy and the high risk of life-threatening infections and disease relapse following hematopoietic stem cell transplantation restrict its application in clinical practice. Thus, there is an urgent need to develop alternative therapeutic tactics with significant efficacy and attenuated adverse effects. Here, we revealed that umbilical cord-derived mesenchymal stem cells (UC-MSC) efficiently induced AML cell differentiation by shuttling the neutrophil elastase (NE)-packaged extracellular vesicles (EVs) into AML cells. Interestingly, the generation and release of NE-packaged EVs could be dramatically increased by vitamin D receptor (VDR) activation in UC-MSC. Chemical activation of VDR by using its agonist 1α,25-dihydroxyvitamin D3 efficiently enhanced the pro-differentiation capacity of UC-MSC and then alleviated malignant burden in AML mouse model. Based on these discoveries, to evade the risk of hypercalcemia, we synthetized and identified sw-22, a novel non-steroidal VDR agonist, which exerted a synergistic pro-differentiation function with UC-MSC on mitigating the progress of AML. Collectively, our findings provided a non-gene editing MSC-based therapeutic regimen to overcome the differentiation blockade in AML

Forest biology project advisory committee meeting

"October 15-16, 1997."Mass clonal propagation of improved conifers - elementals analysis of zygotic female gametophyte and embryo tissues / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - embryo development improvements based on elemental analysis of female gametophyte, zygotic and somatic embryo tissues / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman, Paul Montello, Mike Cunningham ; Combined effects of activated carbon and pH on ionic composition and 2,4-D availability in a tissue culture medium / S.C. Van Winkle, G.S. Pullman ; F010: Mass clonal propagation of softwoods: functional analysis of zygotic embryo germination / Gary Peter ... [et al.] ; Analysis of stage specific bands from differential display of loblolly pine somatic embryos / John Cairney and Barbara Johns ; Analysis of somatic embryo development using differential display I: advancements in techniques / Nanfei Xu, Gerald Pullman and John Cairney ; Analysis of somatic embryo development using differential display II: cloning, sequencing and characterization of genes differentially expressed at different stages of somatic embryos / Nanfei Xu ... [et al.] ; Analysis of embryo development using differential display III: cloning, sequencing and characterization of genes differentially expressed at different stages of zygotic embryos / Nanfei Xu ... [et al.] ; Isolation and characterization of loblolly pine embryo specific vicilin-like storage protein gene; use of this gene as a molecular marker for somatic embryo sorting / Ranjan Perera ... [et al.] ; Applying expertise in flowering research to embryogenesis I: expression of floral homeotic genes in pine embryos / Lin Ge ... [et al.] ; Applying expertise in flowering research to embryogenesis II: isolation and characterization of flower specific (homeotic) genes from loblolly pine, and expression of these genes in vegetative tissues / Ranjan Perera ... [et al.] ; F011: Mass clonal propagation of hardwoods: establishing and improving the zinnia elegans tracheary element differentiation system at IPST / Gary Peter, Karen Floyd, Chris Ricker ; Gene regulation in woody plants I: promoter and 3'UTR effects: regulation of expression of a stress-related gene: promoter and 3'UTR analysis-an update / Luis Destefano-Beltran and John Cairney ; Gene regulation in woody plants II: 5'UTR effects: regulation of expression and gene structure of a down-regulated gene during drought stress / Luis Destefano-Beltran and John Cairney ; Gene regulation in woody plants III: identification of a plant enzyme which activates a regulatory peptide / Luis Destefano ... [et al.] -- Slide Material

Annual program review, Forest Biology, March 25-26, 1997

"March 25-26, 1997."Mass clonal propagation of improved conifers: project F010 / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation and culture survival / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: long-term storage of loblolly pine somatic embryo cultures in liquid nitrogen / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers: field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman, Paul Montello, Mike Cunningham ; Mass clonal propagation of improved conifers: progress in protocol development and targets / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers - elemental analysis of zygotic female gametophyte and embryo tissues / Gerald Pullman, Mike Buchanan, Yolanda Powell ; Mass clonal propagation of improved conifers: antibody markets, straining procedures and image analysis for characterizing pinus taeda embryogenesis / Gary Peter, Levi Barclay ; Mass clonal propagation of improved conifers: construction of cDNA libraries from zygotic embryos of pinus taeda / Gary Peter / Gerald Pullman ; Differential display as a tool to understand and improve somatic embryogenesis / John Cairney ; Monitoring gene expression in somatic embryos of an elite genotype of loblolly pine / John Cairney and Barbara Johns ; Differential display to compare gene expression in loblolly pine zygotic and somatic embryos / Vincent Ciavatta, Jerry Pullman and John Cairney ; Analysis of somatic embryo development using differential display: rapid identification, cloning and sequencing of marker bands by an improved method / Nanfei Xu, Jerry Pullman, and John Cairney ; Preliminary comparison of zygotic and somatic embryo development using differential display / Nanfei Xu, Jerry Pullman and John Cairney ; Gene expression during loblolly pine embryogenesis: drought-inducible genes also expressed in pine cell suspension culture / Ranjan Perera and John Cairney ; Mass clonal propagation of genetically improved and engineered hardwoods: project F011 / Gerald Pullman ... [et al.] ; Agrobacterium tumefaciens-mediated transformation of populus deltoides leaf sections / Ronald J. Dinus ... [et al.] ; Regulation of expression of a stress-related gene: promoter and 3' UTR analysis / Luis Destefano and John Cairney ; Identification of a plant enzyme which activates a regulatory peptide / John Cairney ... [et al.] ; Three year research plan for mass clonal propagation of genetically improved and engineered hardwoods 1997-2000 / Gary Peter, John Cairney, Gerald Pullman -- Slide Material