A new homoisoflavone from <i>Portulaca oleracea</i> L<i>.</i> and its antioxidant activity

<p>A new compound was identified as 3-(2-hydroxybenzyl)-5,7-dimethoxy-4<i>H</i>-chromen-4-one (or 2′-dihydroxy-5,7-dimethoxy-homoisoflavone), oleracone F (<b>1</b>), together with <i>p</i>-hydroxy ethyl cinnamate (<b>2</b>) and 4-hydroxy-3-methoxy ethyl cinnamate (<b>3</b>) isolated from the <i>Portulaca oleracea</i> L. for the first time and six known compounds, salicylic acid (<b>4</b>), <i>β</i>-carboline-3-carboxylic acid (<b>5</b>), aurantiamide (<b>6</b>), portulacanone A (<b>7</b>), B (<b>8</b>) and C (<b>9</b>)<i>.</i> Their structures were determined using spectroscopic methods, including one- and two-dimensional nuclear magnetic resonance and high-resolution electrospray ionization time-of-flight mass spectrometry. Notably, oleracone F (<b>1</b>) presented scavenging activity in 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical quenching assay, with IC₅₀ value of 17.78 μM.</p

Two novel ketone alkaloids from <i>Portulaca oleracea</i>L. and their anti-inflammatory activities

Eleven compounds were obtained from Portulaca oleracea L., including two novel ketone alkaloids, (1, 2), 4-hydroxy-3-methoxybenzamide (3) (isolated for the first time), β-adenosine (4), oleracrylimide A and B (5, 6), oleracein H, C, D, Q and A (7–11). The two novel ketone alkaloids were identified as 5-acetyl-5-methylcyclopent-2-ene-1-carboxamide (1), named oleraciamide H, and (2 R,3S,4R,5R)-5-((R)-1,2-dihydroxyethyl)-3,4-dihydroxytetrahydrofuran-2-yl glycinate (2), named oleracone Q by spectroscopic methods, including 1D, 2D NMR and compound fingerprints. Additionally, their anti-inflammatory activities were tested via RAW 264.7 cells induced by LPS and found that they could significantly inhibit the release of IL-1β and TNF-α.</p

A new lactam alkaloid from <i>Portulaca oleracea</i> L. and its cytotoxity

<p>A new lactam alkaloid named oleraciamide D (<b>1</b>), indentified as (5<i>R</i>)-4-(3-methoxy-4-hydroxyphenyl)-5-(4-hydroxyphenyl)-5,6-dihydropyridin-2(1<i>H</i>)-one, together with five known compounds, indole-3-aldehyde (<b>2</b>), portulacatone (<b>3</b>), <i>N</i>-<i>trans</i>-feruloyloctopamine (<b>4</b>), <i>N</i>-<i>trans</i>-feruloyl-3′-O-methyldopamine (<b>5</b>) and <i>N</i>-<i>trans</i>-feruloyltyramine (<b>6</b>) were isolated from <i>Potulaca oleracea</i> L. Among them, indole-3-aldehyde (<b>2</b>) was isolated from the medicine for the first time. The structure of the new alkaloid was elucidated via UHPLC-ESI-Q-TOF/MS, 1D NMR and 2D NMR. The five known compounds were established by comparing the ¹H-NMR and ¹³C NMR with the reported literature. Oleraciamide D (<b>1</b>) showed cytotoxicity against SH-SY5Y cells when concentration at 50 uM by CCK-8 method.</p

Additional file 1 of Comparative analysis of chloroplast genomes of Pulsatilla species reveals evolutionary and taxonomic status of newly discovered endangered species Pulsatilla saxatilis

Supplementary Material