From Decolonial to the Postcolonial: Trauma of an Unfinished Agenda

Expression stability of the candidate reference genes under different conditions. (DOCX 13 kb

Microarray analysis of expression profiles of selected <i>P. xylostella</i> miRNAs at different developmental stages.

<p>After microarray hybridization and statistical analyses, a set of <i>P. xylostella</i> miRNAs differentially expressed in eggs, larvae, pupae and adults was identified. Two replicates and profiles clustering are presented for miRNAs significantly expressed among developmental stages. Microarray data were analyzed using the TIGR MeV (MultiExperiment Viewer) v4.1 software, <a href="http://www.tm4.org/mev.html" target="_blank">http://www.tm4.org/mev.html</a>, and one-way ANOVA. Color coding: red, up-regulated; black, mean; green, down-regulated.</p

Length distribution of mappable reads obtained from <i>P. xylostella</i> deep sequencing.

<p>Reads with length >25nt were excluded from miRNA mapping.</p

Selected reference genes under different insecticide treatments.

<p>Selected reference genes under different insecticide treatments.</p

Comparative analysis of miRNA sequence similarity between <i>P. xylostella</i> and other insect species.

<p>As expected, <i>P. xylostella</i> miRNAs have the highest sequence similarity with Lepidoptera insects.</p

Known and predicted <i>P. xylostella</i> miRNAs referencing to its own genome.

<p>Known and predicted <i>P. xylostella</i> miRNAs referencing to its own genome.</p

Length distribution and copy number of the <i>P. xylostella</i> miRNAs.

<p>Length distribution and copy number of the <i>P. xylostella</i> miRNAs.</p

Expression levels of candidate reference genes.

<p>The expression level of candidate <i>Bemisia tabaci</i> reference genes in the tested samples is shown in terms of the cycle threshold number (Ct-value). The box plot indicates the mean of duplicate samples; the whiskers indicate the standard error of the mean.</p