Commercial flow cytometer counting results for the mixed RBC and HepG2 cells.

<p>The absolute counts of RBC and HepG2 are 1054 and 978 with the ratio of RBC/HepG2 by 51.9%:48.1% = 1.08: 1.</p

Different contact imaging systems without optical lens.

<p>(A) Static contact imaging system. (B) Microfluidic contact imaging system with capillary flow. (C) The proposed microfluidic contact-imaging cytometer system with continuous flow: (C1) bonding process; (C2) overall system structure.</p

Comparison of concentration measurement results for 6 µm microbead solution between the developed microfluidic cytometer and the commercial flow cytometer.

<p>The average counting result of the developed microfluidic cytometer matched well with that of the commercial cytometer with 8% error.</p

Microfluidic contact-imaging cytometer system for flowing cell detection, recognition and counting.

<p>(A) Cell shadow image by contact imaging. (B) Captured video of flowing cells. (C) CMOS image sensor board schematic with external controls. (D) System board of the developed microfluidic cytometer.</p

ELM-SR on-line testing results for HepG2 and RBC cells.

<p>The resolution is improved by 4× after ELM-SR processing. (A) The HepG2 on-line testing image and the recovered SR image. (B) The RBC on-line testing image and the recovered SR image. (C) The comparison of MSSIM for different SR images obtained under different training models. The detected HepG2 and RBC can be correctly categorized to its type as the SR image recovered by corresponding ELM-SR model produces a larger MSSIM when compared to each cell HR library. The scale bar indicates 5 µm.</p

Comparison of counting results of different microbead concentration solutions between the developed microfluidic cytometer and the commercial flow cytometer.

<p>(A) Measurement results correlate well between the developed system and the commercial one (y = 0.97x-8, correlation coefficient = 0.996). (B) The Bland-Altman analysis of the measurement results between the developed one and the commercial one show a mean bias of −13.6 uL⁻¹, the lower 95% limit of agreement by −61.0 uL⁻¹, and the upper 95% limit of agreement by 33.8 uL⁻¹.</p

Measured RBC and HepG2 counting results of the developed microfluidic cytometer with ELM-SR based cell recognition.

<p>Measured RBC and HepG2 counting results of the developed microfluidic cytometer with ELM-SR based cell recognition.</p

ELM enhanced single-frame super-resolution processing flow.

<p>(A) ELM-SR processing flowchart. The training is performed off-line to generate a reference model that can map the interpolated LR images with the HF components from the HR images; and the testing is performed on-line to recover a SR image from the input LR image with the reference model. (B) Flowing cell recognition flowchart. The detected LR image is processed with ELM-SR to obtain SR images according to different off-line trained models. Then, the SR images are compared with typical HR cell images in the library with cell categorized to one type that has the largest MSSIM.</p

ELM-SR off-line training images for HepG2 and RBC cells.

<p>(A) The original HR images for HepG2 cell with two different appearances; and the same for RBC cells. (B) The corresponding LR images. (C) The interpolated images of LR images, which cannot show HF details. (D) The extracted HF components. The scale bar indicates 5 µm.</p