Additional file 8: Table S1. of GLI pathogenesis-related 1 functions as a tumor-suppressor in lung cancer

List of primers used for RT-PCR analysis. (PDF 40 kb

Additional file 3: Figure S3. of GLI pathogenesis-related 1 functions as a tumor-suppressor in lung cancer

The expression profile of WDR77 in various lung cancer cell lines. The results were obtained from expression profiling (GDS1688) of a set of 29 lung cancer cell lines consisting of ten non-small cell adenocarcinoma, ten small cell cancer, and nine squamous cell cancer lines. Value: the RMA normalized expression value. Rank: the position of the WDR77 gene across 22,337 genes on the DNA chip based on the expression level (from low to high). (PDF 428 kb

Additional file 1: Figure S1. of GLI pathogenesis-related 1 functions as a tumor-suppressor in lung cancer

GLIPR1 expression in A549, PC14, LNCaP, PC3 and U87 cells. Western blot analysis of whole-cell lysates derived from A549 (lane 1), PC14 (lane2), LNCaP (lane 3), PC3 (lane 4), and U87 (lane 5) cells with anti-GLIPR1 or -actin antibody. (PDF 308 kb

Mxenes–Au NP Hybrid Plasmonic 2D Microplates in Microfluidics for SERS Detection

Combined with microfluidics, surface-enhanced Raman spectroscopy (SERS) exhibits huge application prospective in sensitive online detection. In current studies, the design and optimization of plasmonic enhanced structures in microfluidics for SERS detection could be an interesting challenge. In this work, hybrid plasmonic 2D microplates composed of Mxenes (Ti3C2Tx) microplates and in-situ synthesized Au nanoparticles (Au NPs) are fabricated in a microchannel for enhanced structures in SERS microfluidics. Benefiting from the 2D Mxenes microplates with complex distributions, the enhanced areas generated by Au NPs are quite enlarged in a microchannel, which exhibits high sensitivity in SERS detection at 10−10 M for Nile blue (NB) molecules in microfluidics. The mechanism of electromagnetic enhancement (EM) and chemical enhancement (CM) is analyzed. The experimental data indicate the ultrasonic times of Mxenes and the concentration of Au3+ play important roles in the sensitivity of SERS detection, which is confirmed by the simulated electric field distributions. Furthermore, a typical pesticide (thiram) at 100 ppm in water is detected on these SERS microfluidics with hybrid plasmonic enhanced structures, which demonstrates that our work not only strengthens the knowledge of plasmonics but also enlarges the application of SERS

Correlation between preoperative peripheral blood NLR, PLR, LMR and prognosis of patients with head and neck squamous cell carcinoma

Abstract Background Markers that can be used to evaluate the prognosis of patients with head and neck squamous cell carcinoma (HNSCC) remain undefined. Objective This study aimed to investigate the prognostic impact of preoperative neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-to-monocyte ratio (LMR) in patients with HNSCC who underwent surgery-based treatment for the first time. Methods This retrospective study included patients HNSCC who underwent surgery-based treatment at our institution between January 2018 and December 2020. Specificity and sensitivity were analyzed using receiver operating characteristic (ROC) curves and the critical value was determined. Patients were divided into low and high groups according to NLR, PLR, and LMR the critical value. Log-rank and Cox proportional hazards models were used to evaluate the associations between preoperative NLR, PLR, LMR, and overall survival (OS). Results A total of 304 patients with HNSCC were included, of whom 190 (62.5%) and 114 (37.5%), 203 (66.8%) and 101 (33.2%), 98 (32.2%), and 206 (67.8%) cases were classified as low NLR and high NLR groups, low PLR and high PLR groups, and low LMR and high LMR groups, respectively. Univariate analysis showed that white blood cell count (WBC), neutrophil count (NEU), platelet count (PLT), NLR, pathologic N stage (pN stage), TNM stage and postoperative complications were significantly associated with OS (p < 0.05). Multivariate analysis showed that NEU, NLR, TNM stage and postoperative complications were independent negative prognostic factors for HNSCC (p < 0.05). Conclusion Preoperative NLR is an independent negative prognostic factor for HNSCC. Patients with an increased NLR may have a poor OS