Effects of Cobalt Compounds on the Morphology and Structure of Carbonaceous Materials Prepared by Hydrothermal/Solvothermal Carbonization of Furfural

The cobalt-assisted hydrothermal/solvothermal carbonization of furfural has been developed into a facile and versatile strategy to prepare structured carbonaceous materials. In this paper, the hierarchical flower-like, honeycomb-like, and spherical particles can be readily obtained in the presence of different cobalt compounds. Also, it was demonstrated that the developing of hierarchical carbonaceous structures was strongly influenced by the formation of cobalt crystals in products during the reaction. We believe that the cobalt crystals could act as a kind of structure-directing agent to induce the fabrication of hierarchical carbonaceous structures. Organic cobalt compound was indicative of beneficial cobalt precursor for synthesis of hierarchical carbonaceous structures. The presence of ethanol facilitated the aggregation and assembly of primary nanoparticles to form hierarchical structures

Selection and Characterization of Aptamers against Salmonella typhimurium Using Whole-Bacterium Systemic Evolution of Ligands by Exponential Enrichment (SELEX)

In this paper, a high-affinity ssDNA aptamer binding to Salmonella typhimurium was obtained by a whole-bacterium-based Systemic Evolution of Ligands by Exponential Enrichment (SELEX) procedure. After nine rounds of selection with <i>S. typhimurium</i> as the target, a highly enriched oligonucleotide pool was sequenced and then grouped into different families based on primary sequence homology and secondary structure similarity. Eleven sequences from different families were selected for further characterization via flow cytometry analysis. The results showed that the sequence ST2P demonstrates affinity for <i>S. typhimurium</i> much more strongly and specifically than other sequences tested. The estimated <i>K</i>_d value of this particularly promising aptamer was 6.33 ± 0.58 nM. To demonstrate the potential use of the aptamers in the quantitative determination of <i>S. typhimurium</i>, a fluorescent bioassay with the aptamer ST2P was prepared. Under optimal conditions, the correlation between the concentration of <i>S. typhimurium</i> and fluorescent signal was found to be linear within the range of 50–10⁶ cfu/mL (<i>R</i>² = 0.9957). The limit of detection (LOD) of the developed method was found to be 25 cfu/mL. This work demonstrates that this aptamer could potentially be used to improve the detection of <i>S. typhimurium</i>

IGF2R expression in vitro after overexpression or knockdown of let-7g-5p.

<p>(a) Overexpression of let-7g-5p in HEK293T and hESCs cells. Cells were cultured and incubated with let-7g-5p mimic or negative control. Levels of let-7g-5p were determined using qPCR. (b) Knockdown of let-7g-5p miRNA by transfection with hsa-let-7g-5p inhibitor. Cells were cultured and incubated with hsa-let-7g-5p inhibitor or negative control. Levels of let-7g-5p were determined using qPCR. (c-d) Expression of <i>IGF2R</i> mRNA, as determined using qPCR analysis. Experiments were replicated three times, and error bars represent standard error. *P<0.05, **P<0.01 ***P<0.001.</p

Kyoto Encyclopedia of Genes and Genomes analysis indicating the number of miRNA-targeted genes and the various associated pathways.

<p>Horizontal axis indicates the annotated signaling pathways identified, and the vertical axis indicates the number of genes in each pathway to which miRNAs were predicted to bind.</p

IGF2BP-1 expression in vitro after overexpression or knockdown of let-7f-5p.

<p>(a) Overexpression of let-7f-5p in HEK293T and hESCs cells. Cells were cultured and incubated with hsa-let-7f-5p mimic or negative control. Levels of let-7f-5p were determined using qPCR. (b) Knockdown of let-7f miRNA by transfection with hsa-let-7f-5p inhibitor. Cells were cultured and incubated with hsa-let-7f-5p inhibitor or negative control. Levels of let-7f-5p were determined using qPCR. (c-d) Expression of <i>IGF2BP-1</i> mRNA, as determined using qPCR analysis. Experiments were replicated three times, and error bars represent standard error. **P<0.01, ***P<0.001.</p

MicroRNA Profiles in Spontaneous Decidualized Menstrual Endometrium and Early Pregnancy Decidua with Successfully Implanted Embryos - Fig 3

<p>(a) Let-7f-5p was predicted to bind to a site on the <i>IGF2BP-1</i> mRNA. (b) Let-7g-5p was predicted to bind to a site on the <i>IGF-2R</i> mRNA.</p

Validation of the differential expression of six known miRNAs by qPCR analysis.

<p>The miRNA expression level was normalized to that of U6 snRNA. “*” represents P value <0.05, “**” represents P value <0.01, and “***” represents P value <0.001. All experiments were repeated with 3 replicates.</p

The 62 novel miRNAs expressed in the human decidua and menstrual endometrium.

<p>Candidates with sequence overlap with known miRNAs (but having a distinct mature miRNA sequence: isomiRs) and sequence alignments of novel miRNA candidates with known miRNAs of other species are shown.</p

Whole-exome sequencing reveals novel variants associated with abnormal uterine bleeding caused by copper intrauterine device Supplementary Materials

Aim: This study aimed to explore the genetic risk factors and validate variants of abnormal uterine bleeding after copper intrauterine device insertion. Methods: Whole-exome sequencing was performed and several variants were validated by Sequenom MassARRAY. Results: Eight variants showed potential clinical damage according to American College of Medical Genetics and Genomics criteria. By combined analysis of screening and validation, NFASC RS2802808 C>G p.Ile971Met (Pallele = 0.009 and Pgenotype = 0.027) and PIGR RS2275531 C>T p.Gly365Ser (Pallele = 0.009 and Pgenotype = 0.013) variants were identified as significantly associated with abnormal uterine bleeding with a false discovery rate and PIGR may play a role in abnormal uterine bleeding by regulating coagulation fibrinolysis and endometrial epithelium inflammation functions. Conclusion: These findings provide a genetic basis for clinical individualization and precision of intrauterine device implantation.</p