Histopathological alterations in the eWAT of mice fed HFD or/and PFOA.

<p>Adult mice were pair-fed a high-fat diet (HFD) or low fat control diet (CTRL) with or without perfluorooctanoic acid (PFOA) administration at 5 mg/kg/day for 3 weeks. H&E staining of Epididymal white adipose tissue (eWAT). Arrows: Inflammatory cell infiltration.</p

Fold changes of hepatic metabolites in mice fed HFD or/and PFOA exposure for 3 weeks.

<p>Hepatic metabolites were analyzed by GC-TOF MS and HPLC-TOFMS (n = 7–8).</p>a<p>Fold change (FC) was obtained by comparing those metabolites in perfluorooctanoic acid (PFOA) group, high fat diet (HFD) group and HFD-PFOA group to control group (CTRL) or HFD-PFOA group to PFOA group; FC with a value >1 indicates a relatively higher concentration present in PFOA group, HFD group or HFD-PFOA group while a value <1 means a relatively lower concentration as compared to the controls or a relatively higher concentration present in HFD-PFOA group while a value <1 means a relatively lower concentration as compared to the PFOA group.</p>b<p><i>p</i> values from Student’s <i>t</i>-test.</p

Fold changes of proinflammatory cytokine genes in the liver of mice subjected to HFD or/and PFOA exposure for 3 weeks.

<p>The mRNA levels were analyzed by real time RT-PCR (<i>n</i> = 3, assay replicates = 4).</p>*<p>Significant differences (<i>p</i><0.05) were determined by Student’s <i>t</i>-test. CTRL: Control. HFD: High fat diet. HFD-PFOA: High fat diet plus PFOA.</p

Primer sequences used for qPCR analysis.

<p>Primer sequences used for qPCR analysis.</p

Fold changes 0f drug metabolism genes in the liver of mice subjected to HFD or/and PFOA exposure for 3 weeks.

<p>The mRNA levels were analyzed by RT² Profiler™ PCR Array (<i>n</i> = 3), and genes with ≥1.5 fold change are listed.</p>*<p>Significant differences (<i>p</i><0.05) were determined by Student’s <i>t</i>-test. CTRL: Control. HFD: High fat diet. HFD-PFOA: High fat diet plus PFOA.</p

Gene expression profiles in the liver of mice fed HFD or/and PFOA.

<p>Adult mice were pair-fed a high-fat diet (HFD) or low fat control diet (CTRL) with or without perfluorooctanoic acid (PFOA) administration at 5 mg/kg/day for 3 weeks. The mRNA levels were analyzed by RT² Profiler™ PCR Array (<i>n</i> = 3). <b>A</b>. Genes related to fatty acid metabolism. <b>B</b>. Genes related to drug metabolism. <b>C</b>. Genes related to glucose metabolism. Red and green corresponds to average up- and down-regulation, respectively.</p

Fold changes of serum metabolites in mice subjected to HFD or/and PFOA exposure for 3 weeks.

<p>Serum metabolites were analyzed by GC-TOF MS and HPLC-TOFMS (n = 7–8).</p>a<p>Fold change (FC) was obtained by comparing those metabolites in perfluorooctanoic acid (PFOA) group, high fat diet (HFD) group and HFD-PFOA group to control group (CTRL) or HFD-PFOA group to PFOA group; FC with a value >1 indicates a relatively higher concentration present in PFOA group, HFD group or HFD-PFOA group while a value <1 means a relatively lower concentration as compared to the controls or a relatively higher concentration present in HFD-PFOA group while a value <1 means a relatively lower concentration as compared to the PFOA group.</p>b<p><i>p</i> values from Student’s <i>t</i>-test.</p

Body weights, organ weights and blood parameters in mice subjected to HFD or/and PFOA exposure for 3 weeks.

<p>Data are means ± SD (n = 7–8). Significant differences (<i>p</i><0.05) among a, b, c, d were determined by One-Way ANOVA with Dunnett’s post hoc comparison. eWAT: Epididymal white adipose tissue. sWAT: Subcutaneous white adipose tissue. BW: Body weight. FFAs: Free fatty acids.</p

Additional file 1 of Long-term outcomes with HLX01 (HanliKang®), a rituximab biosimilar, in previously untreated patients with diffuse large B-cell lymphoma: 5-year follow-up results of the phase 3 HLX01-NHL03 study

Supplementary Material

Additional file 1: of Chidamide in relapsed or refractory peripheral T cell lymphoma: a multicenter real-world study in China

Methods (DOCX 16 kb