Phylogenetic network analysis of <i>Toxoplasma gondii</i> isolates from humans and animals in China.

<p>From the 23 samples, 5 genotypes were identified. Genotype number (ToxoDB PCR-RFLP #) and the representative strains are listed for each taxonomic branch. The representative strains from each genotype were underlined found in this study. The numbers in parentheses indicate the number of isolates from this study belonging to that genotype. The phylogenetic network analysis with EF1, HP2, UPRT1, UPRT7, GRA6 and GRA7 shows a perfect match with PCR-RFLP allele types at 10 multilocus markers used in the present study.</p

Multilocus PCR-RFLP genotyping of <i>T. gondii</i> from animals and humans in China.

a<p>μ−1 and μ−2 represent unique RFLP genotypes, respectively.</p

The genotypes and geographic regions of <i>T. gondii</i> Chinese isolates.

<p>Panel A: a map of China to show the cumulative data of geographic distribution where the Chinese isolates of <i>T. gondii</i> used for genotyping were collected. The provinces and cities where <i>T. gondii</i> isolates were obtained and indicated by red dots and black dots, respectively. Red dots represent the locations (counties) where the isolates were collected in this work. Black dots indicate the locations (counties) where the isolates were collected in the early studies. SX: Shanxi province; SD: Shandong province; HN: Henan province; AH: Anhui province; JS: Jiangsu province; HuB: Hubei province; HuN: Hunan province; GD: Guangdong province; ZJ: Zhejiang province; FJ: Fujian province; YN: Yunnan province; QH: Qinghai province; GS: Gansu province; XJ: Xinjiang province; BJ: Beijing city; SH: Shanghai city. Panel B: a: the cumulative data of genotypes of <i>T. gondii</i> Chinese isolates. b: the genotypes of <i>T. gondii</i> isolates from human patients in mainland China. This is the first report of the genotype ToxoDB#204 from humans.</p

The kinetics of infection in mice with TgCtwh6 isolates.

<p>Kinetics of infection in blood and organs after oral infection with 50 cysts of TgCtwh6 isolate (genotype Chinese 1) in mice. I: the number of DNA copies in blood and tissues of the TgCtwh6 isolate post infection at various intervals by qPCR. Each point represents the mean value of the <i>T. gondii</i> DNA copies for five mice ± SD. II: detection of PCR products of <i>T. gondii</i> 529 bp fragments extracted from brain or ascitic fluid in the recipient mice. Abbreviations: <b>A</b>; blood, <b>B</b>; heart, <b>C</b>; liver, <b>D</b>; brain, and <b>E</b>; lymph node. b, n, and p represent blank, negative and positive control. *<i>P</i><0.05.</p

Summary of mouse virulence of representative strains with differents genotypes from China.

<p>Summary of mouse virulence of representative strains with differents genotypes from China.</p

The medical data from patients with toxoplasmosis.

a<p>F, female; M, male;</p>b<p>IC, immunocompromised; HCC, hepato-cellular carcinoma;</p