Effects of different carcass preservation temperatures on oocyte meiotic process.

<p>(A) The number of GV oocytes collected from mouse carcass after preserved for 4 hours at different temperatures. (B) The percentage of GVBD oocytes and PBE in PMI 4 h group at different preservation temperatures. Each bar represents mean ±SEM (n = 3). * P<0.05.</p

Effects of preservation time and temperature on mitochondrial distribution and GSH level in mouse oocytes.

<p>(A) Mitochondrial distribution was evaluated by fluorescence microscopy. Three localization patterns were observed: perinuclear, homogenous and clustered. (B) The percentage of abnormal mitochondrial distribution in GV oocytes in the control group (n = 279) and preservation group at different temperatures. * indicate statistically significant differences (P<0.05). (C) The GSH concentrations in GV oocytes (n = 30) in different groups.</p

Effects of different preservation periods and temperatures on oocyte spindle configuration after IVM.

<p>Oocytes cultured in M2 medium for 8h (MI) and 12h (MII), spindle was stained with FITC-α-tubulin and DNA was stained with PI. (A, B,C) the normal spindle in mouse MI and MII oocyte in control group and experimental group at different preservation temperatures.</p