{N,N-Dimethyl-N′-[phen­yl(2-pyrid­yl)methyl­ene]ethane-1,2-diamine-κ3 N,N′,N′′}dithio­cyanato-κN,κS-copper(II)

In the title complex, [Cu(NCS)2(C16H19N3)], the CuII atom is coordinated by a total of four N atoms; three from one tridentate Schiff base ligand and one from one of the NCS− ions. The S atom from the other NCS− ion completes the distorted square-pyramidal coordination

Bis(N′-benzoyl­pyridine-4-carbohydrazide)(1,10-phenanthroline)copper(II) dinitrate

In the title complex, [Cu(C13H11N3O2)2(C12H8N2)](NO3)2, the CuII atom (site symmetry 2) is coordinated by four N atoms from one 1,10-phenanthroline and two hydrazine ligands, respectively. The hydrazine ligands coordinate to the CuIIatom by a pyridine N atom. These four atoms form a slightly distorted square-planar N4 donor set. In the packing, two additional Cu⋯O inter­actions occur [Cu⋯O = 2.462 (2) Å], resulting in a typical Jahn–Teller-distorted octahedral environment around the Cu atom. N—H⋯O hydrogen bonds result in a three-dimensional network. The O atoms of the anion are disordered over two positions in a 0.68 (2):0.32 (2) ratio

Aqua[N-(2,5-dihydroxybenzyl)imino­diacetato]copper(II)

The title complex, [Cu(C11H11NO6)(H2O)], contains a CuII atom in a distorted square-pyramidal geometry. The metal centre is coordinated in the basal sites by one water mol­ecule and two carboxyl­ate O atoms and one N atom of the tetra­dentate ligand [Cu—O range, 1.9376 (11)–1.9541 (12), Cu—N, 1.9929 (12) Å] while the apical site is occupied by a hydro­quinone O donor atom [Cu—O, 2.3746 (12) Å]. Inter­molecular hydrogen bonding inter­actions involving both hydro­quinone hydr­oxy groups and the coordinated water as donors give a three-dimensional framework structure

Comparison of corneal flap thickness using a FS200 femtosecond laser and a moria SBK microkeratome

<b>AIM:</b> To evaluate differences in flap thickness resulting from use of an Alcon Wavelight FS200 femtosecond laser and a MORIA SBK microkeratome when making a 110-μm-thick corneal flap and to identify the potential factors that affect corneal flap thickness.<b>METHODS:</b> A prospective case study was performed on 120 eyes of 60 patients who were divided into two groups for LASIK, each group consisting of 60 eyes (30 patients). The corneal flaps were created using an Alcon Wavelight FS200 femtosecond laser or a MORIA SBK microkeratome. The central corneal flap thickness was calculated by subtraction pachymetry. Age, central corneal thickness (CCT), spherical equivalent refraction, mean keratometry, and corneal diameter were recorded preoperatively for analysis.<b>RESULTS:</b> Cutting of all flaps was easily performed without intraoperative complications. In the Alcon Wavelight FS200 femtosecond lasergroup, the mean right and left corneal flap thicknesses were 114.0±6.6 μm (range:98-126) and 111.4±7.6 μm (range:98-122), respectively. The difference (2.6±9.1 μm) in the corneal flap thickness between the right and left eyes was not significant (<i>t</i>=1.59, <i>P</i>=0.12). Stepwise regression analysis indicated that the resulting corneal flap thickness was unrelated to the patient’s age, preoperative CCT, spherical equivalent refraction, mean keratometry, or corneal diameter. In the MORIA SBK microkeratome group, the mean right and left corneal flap thicknesses were 110.6±7.4 μm (range:97-125 μm) and 108.2±6.1 μm (range:78-123 μm), respectively. The difference in the corneal flap thickness between the right and left eyes (2.4±6.5μm) was not significant (<i>t</i>=2.039, <i>P</i>=0.0506). The corneal flap thickness was positively correlated with the preoperative CCT through stepwise regression analysis (<i>r</i>=0.297,<i> P</i>=0.021). The corneal flap thickness was not related to age, spherical equivalent refraction, mean keratometry, or corneal diameter. The corneal flap thickness was estimated using the following equation:Tflap=67.77+0.076 CCT (<i>F</i>=5.63, <i>P</i>=0.021).<b>CONCLUSION:</b>Both the Alcon Wavelight FS200 femtosecond laser and the MORIA SBK microkeratome produced 110-μm-thick corneal flaps. The central corneal flap thickness was positively correlated with the preoperative CCT in MORIA SBK microkeratome surgery

Cationic liposomes induce cytotoxicity in HepG2 via regulation of lipid metabolism based on whole-transcriptome sequencing analysis

Abstract Backgroud Cationic liposomes (CLs) can be used as non-viral vectors in gene transfer and drug delivery. However, the underlying molecular mechanism of its cytotoxicity has not been well elucidated yet. Methods We herein report a systems biology approach based on whole-transcriptome sequencing coupled with computational method to identify the predominant genes and pathways involved in the cytotoxicity of CLs in HepG2 cell line. Results Firstly, we validated the concentration-dependent cytotoxicity of CLs with an IC50 of 120 μg/ml in HepG2 exposed for 24 h. Subsequently, we used whole-transcriptome sequencing to identify 220 (77 up- and 143 down-regulated) differentially expressed genes (DEGs). Gene ontology (GO) and pathway analysis showed that these DEGs were mainly related to cholesterol, steroid, lipid biosynthetic and metabolic processes. Additionally, “key regulatory” genes were identified using gene act, pathway act and co-expression network analysis, and expression levels of 11 interested altered genes were confirmed by quantitative real time PCR. Interestingly, no cell cycle arrest was observed through flow cytometry. Conclusions These data are expected to provide deep insights into the molecular mechanism of CLs cytotoxicity.https://deepblue.lib.umich.edu/bitstream/2027.42/144776/1/40360_2018_Article_230.pd

A meta-analysis of the efficacy of azithromycin and benzathine penicillin in early syphilis

Purpose: To systematically analyze the efficacy of azithromycin and benzathine penicillin in early syphilis, and provide guidance for diagnosis and treatment.Methods: Databases were searched for randomized controlled trials (RCTs) and control studies according to keywords, and inclusion and exclusion criteria. Related documents and meeting records were also searched manually to extract study types, basic information of study objects, intervention measurements and study results, and evaluation of the quality of the methodology used.Results: Three studies were excluded from the review. The quality evaluation was B grade, and heterogenicity was good. We adopted a fixed effect model to conduct the meta-analysis. There was no significant difference in the cure rate between azithromycin and benzathine penicillin administered for 6 months. The ORs for 3 time points were 0.96 (95% CI = 0.71, 1.29), 0.01 (95% CI = -0.05, 0.06), and 0.04 (95% CI = -0.02, 0.11; p &lt; 0.05). There were no significant differences in the incidence of adverse events among the three studies.Conclusion: There was no apparent difference in the efficacy of azithromycin and benzathine penicillin in early syphilis. The advantages of azithromycin included good compliance, a long half-life, and a high economic benefit.Keywords: Early syphilis, Azithromycin, Benzathine penicillin, Curative effec