22 research outputs found
Control of the Coordination Status of the Open Metal Sites in Metal–Organic Frameworks for High Performance Separation of Polar Compounds
Metal–organic frameworks (MOFs) with open metal
sites have
great potential for enhancing adsorption separation of the molecules
with different polarities. However, the elution and separation of
polar compounds on such MOFs packed columns using nonpolar solvents
is difficult due to too strong interaction between polar compounds
and the open metal sites. Here, we report the control of the coordination
status of the open metal sites in MOFs by adjusting the content of
methanol (MeOH) in the mobile phase for fast and high-resolution separation
of polar compounds. To this end, high-performance liquid chromatographic
separation of nitroaniline, aminophenol and naphthol isomers, sulfadimidine,
and sulfanilamide on the column packed with MIL-101Â(Cr) possessing
open metal sites was performed. The interaction between the open metal
sites of MIL-101Â(Cr) and the polar analytes was adjusted by adding
an appropriate amount of MeOH to the mobile phase to achieve the effective
separation of the polar analytes due to the competition of MeOH with
the analytes for the open metal sites. Fourier transform infrared
spectra and X-ray photoelectron spectra confirmed the interaction
between MeOH and the open metal sites of MIL-101Â(Cr). Thermodynamic
parameters were measured to evaluate the effect of the content of
MeOH in the mobile phase on the separation of polar analytes on MIL-101Â(Cr)
packed column. This approach provides reproducible and high performance
separation of polar compounds on the open metal sites-containing MOFs
Ratiometric Fluorescent Detection of Phosphate in Aqueous Solution Based on Near Infrared Fluorescent Silver Nanoclusters/Metal–Organic Shell Composite
Synthesis of near-infrared (NIR)
fluorescent AgNCs with high quantum yield and stability is challenging
but important for sensing and bioimaging application. Here, we report
the fabrication of AgNCs/metal–organic shell composite via
the deposition of metal–organic (zinc–nitrogen) coordination
shell around AgNCs for ratiometric detection of phosphate. The composite
exhibits NIR emission at 720 nm with 30 nm red-shift in comparison
to bare AgNCs and a weak emission at 510 nm from the shell. The absolute
quantum yield of NIR fluorescence of the composite is 15%, owing to
FRET from the shell to the AgNCs core under the excitation at 430
nm. Besides, the composite is stable due to the protection of the
shell. On the basis of the composite, a novel ratiometric fluorescence
probe for the detection of phosphate in aqueous solution with good
sensitivity and selectivity was developed. The limit of detection
(3<i>s</i>) is 0.06 ÎĽM, and the relative standard
deviation for 10 replicate detections of 10 ÎĽM phosphate was
0.6%. The recoveries of spiked phosphate in water, human urine, and
serum samples ranged from 94.1% to 103.4%
Self-Assembly of Folate onto Polyethyleneimine-Coated CdS/ZnS Quantum Dots for Targeted Turn-On Fluorescence Imaging of Folate Receptor Overexpressed Cancer Cells
Folate receptor (FR) can be overexpressed by a number
of epithelial-derived
tumors, but minimally expressed in normal tissues. As folic acid (FA)
is a high-affinity ligand to FR, and not produced endogenously, development
of FA-conjugated probes for targeted imaging FR overexpressed cancer
cells is of significance for assessing cancer therapeutics and for
better understanding the expression profile of FR in cancer. Here
we report a novel turn-on fluorescence probe for imaging FR overexpressed
cancer cells. The probe was easily fabricated via electrostatic self-assembly
of FA and polyethyleneimine-coated CdS/ZnS quantum dots (PEI-CdS/ZnS
QDs). The primary fluorescence of PEI-CdS/ZnS QDs turned off first
upon the electrostatic adsorption of FA onto PEI-CdS/ZnS QDs based
on electron transfer to produce negligible fluorescence background.
The presence of FR expressed on the surface of cancer cells then made
FA desorb from PEI-CdS/ZnS QDs due to specific and high affinity of
FA to FR. As a result, the primary fluorescence of PEI-CdS/ZnS QDs
adhering to the cells turned on due to the inhibition of electron
transfer. The most important merits of the developed probe are its
simplicity and the effective avoidance of the false positive results
due to the simple electrostatic self-assembly of FA onto the surface
of PEI-CdS/ZnS QDs and the involved fluorescence “off-on”
mechanism. The probe was demonstrated to be sensitive and selective
for targeted imaging of FR overexpressed cancer cells in turn-on mode
Fabrication of Transferrin Functionalized Gold Nanoclusters/Graphene Oxide Nanocomposite for Turn-On Near-Infrared Fluorescent Bioimaging of Cancer Cells and Small Animals
Transferrin (Tf)-functionalized gold nanoclusters (Tf-AuNCs)/graphene
oxide (GO) nanocomposite (Tf-AuNCs/GO) was fabricated as a turn-on
near-infrared (NIR) fluorescent probe for bioimaging cancer cells
and small animals. A one-step approach was developed to prepare Tf-AuNCs
via a biomineralization process with Tf as the template. Tf acted
not only as a stabilizer and a reducer but also as a functional ligand
for targeting the transferrin receptor (TfR). The prepared Tf-AuNCs
gave intense NIR fluorescence that can avoid interference from biological
media such as tissue autofluorescence and scattering light. The assembly
of Tf-AuNCs and GO gave the Tf-AuNCs/GO nanocomposite, a turn-on NIR
fluorescent probe with negligible background fluorescence due to the
super fluorescence quenching property of GO. The NIR fluorescence
of the Tf-AuNCs/GO nanocomposite was effectively restored in the presence
of TfR, due to the specific interaction between Tf and TfR and the
competition of TfR with the GO for the Tf in Tf-AuNCs/GO composite.
The developed turn-on NIR fluorescence probe offered excellent water
solubility, stability, and biocompatibility, and exhibited high specificity
to TfR with negligible cytotoxicity. The probe was successfully applied
for turn-on fluorescent bioimaging of cancer cells and small animals
Zeolitic Imidazolate Framework‑8 for Fast Adsorption and Removal of Benzotriazoles from Aqueous Solution
1<i>H</i>-benzotriazole (BTri) and 5-tolyltriazole (5-TTri) are
emerging pollutants; the development of novel materials for their
efficient adsorption and removal is thus of great significance in
environmental sciences. Here, we report the application of zeolitic
imidazolate framework-8 (ZIF-8) as a novel adsorbent for fast removal
of BTri and 5-TTri in aqueous solution in view of adsorption isotherms,
kinetics and thermodynamics, desorption, and adsorbent regeneration.
The adsorption of BTri and 5-TTri on ZIF-8 was very fast, and most
of BTri and 5-TTri were adsorbed in the first 2 min. The adsorption
for BTri and 5-TTri follows a pseudo-second-order kinetics and fits
the Langmuir adsorption model with the adsorption capacity of 298.5
and 396.8 mg g<sup>–1</sup> for BTri and 5-TTri at 30 °C,
respectively. The adsorption was a spontaneous and endothermic process
controlled by positive entropy change. No remarkable effects of pH,
ionic strength, and dissolved organic matter on the adsorption of
BTri and 5-TTri on ZIF-8 were observed. The used ZIF-8 could be regenerated
effectively and recycled at least three times without significant
loss of adsorption capacity. In addition, ZIF-8 provided much larger
adsorption capacity and faster adsorption kinetics than activated
carbon and ZIF-7. The hydrophobic and π–π interaction
between the aromatic rings of the BTri and 5-TTri and the aromatic
imizole rings of the ZIF-8, and the coordination of the nitrogen atoms
in BTri and 5-TTri molecules to the Zn<sup>2+</sup> ions in the ZIF-8
framework was responsible for the efficient adsorption. The fast adsorption
kinetics, large adsorption capacity, excellent reusability as well
as the pH, ionic strength, and dissolved organic matter insensitive
adsorption create potential for ZIF-8 to be effective at removing
benzotriazoles from aqueous solution
Liposome-Coated Persistent Luminescence Nanoparticles as Luminescence Trackable Drug Carrier for Chemotherapy
Near-infrared
persistent luminescence nanoparticles (NIR-PLNPs)
are promising imaging agents due to deep tissue penetration, high
signal-to-noise ratio, and repeatedly charging ability. Here, we report
liposome-coated NIR-PLNPs (Lipo-PLNPs) as a novel persistent luminescence
imaging guided drug carrier for chemotherapy. The Lipo-PLNP nanocomposite
shows the advantages of superior persistent luminescence and high
drug loading efficiency and enables autofluorescence-free and long-term
tracking of drug delivery carriers with remarkable therapeutic effect
Biomimetic Persistent Luminescent Nanoplatform for Autofluorescence-Free Metastasis Tracking and Chemophotodynamic Therapy
Metastasis
is the main cause of death in people with cancer. Early
diagnosis and targeted therapy for metastasis is crucial for the survival
of the cancer patients. However, metastasis is hard to trace for its
small size, dispersed distribution and unvascularized anatomy. Here
we report a biomimetic persistent luminescent nanoplatform for noninvasive
high-sensitive diagnosis and 808 nm laser controlled photodynamics
assisted chemotherapy of metastasis. The nanoplatform is composed
of a photosensitizer functionalized persistent luminescent nanoparticle
core, a doxorubicin loaded hollow silica interlayer and a cancer cell
membrane shell for effective metastasis theranostic. The cancer cell
membrane shell prevents drug leakage and endows the nanoplatform with
targeting ability to metastasis. The reactivatable persistent luminescence
of persistent luminescent nanoparticles not only enables long-term
in vivo metastasis tracking, but also provides internal light source
for singlet oxygen generation to kill cancer cells and further break
the membrane shell for drug release. This work provides a promising
strategy to develop persistent luminescence imaging guided theranostic
nanoplatforms for early metastasis
Fluorescent Metal–Organic Framework MIL-53(Al) for Highly Selective and Sensitive Detection of Fe<sup>3+</sup> in Aqueous Solution
Fluorescent
metal–organic frameworks (MOFs) have received
great attention in sensing application. Here, we report the exploration
of fluorescent MIL-53Â(Al) for highly selective and sensitive detection
of Fe<sup>3+</sup> in aqueous solution. The cation exchange between
Fe<sup>3+</sup> and the framework metal ion Al<sup>3+</sup> in MIL-53Â(Al)
led to the quenching of the fluorescence of MIL-53Â(Al) due to the
transformation of strong-fluorescent MIL-53Â(Al) to weak-fluorescent
MIL-53Â(Fe), allowing highly selective and sensitive detection of Fe<sup>3+</sup> in aqueous solution with a linear range of 3–200
ÎĽM and a detection limit of 0.9 ÎĽM. No interferences from
0.8 M Na<sup>+</sup>; 0.35 M K<sup>+</sup>; 11 mM Cu<sup>2+</sup>;
10 mM Ni<sup>2+</sup>; 6 mM Ca<sup>2+</sup>, Pb<sup>2+</sup>, and
Al<sup>3+</sup>; 5.5 mM Mn<sup>2+</sup>; 5 mM Co<sup>2+</sup> and
Cr<sup>3+</sup>; 4 mM Hg<sup>2+</sup>, Cd<sup>2+</sup>, Zn<sup>2+</sup>, and Mg<sup>2+</sup>; 3 mM Fe<sup>2+</sup>; 0.8 M Cl<sup>–</sup>; 60 mM NO<sub>2</sub><sup>–</sup> and NO<sub>3</sub><sup>–</sup>; 10 mM HPO<sub>4</sub><sup>2–</sup>, H<sub>2</sub>PO<sub>4</sub><sup>–</sup>, SO<sub>3</sub><sup>2–</sup>, SO<sub>4</sub><sup>2–</sup>, and HCOO<sup>–</sup>; 8 mM CO<sub>3</sub><sup>2–</sup>, HCO<sub>3</sub><sup>–</sup>, and C<sub>2</sub>O<sub>4</sub><sup>2–</sup>; and 5 mM CH<sub>3</sub>COO<sup>–</sup> were found for the detection of 150
ÎĽM Fe<sup>3+</sup>. The possible mechanism for the quenching
effect of Fe<sup>3+</sup> on the fluorescence of MIL-53Â(Al) was elucidated
by inductively coupled plasma-mass spectrometry, X-ray diffraction
spectrometry, and Fourier transform infrared spectrometry. The specific
cation exchange behavior between Fe<sup>3+</sup> and the framework
Al<sup>3+</sup> along with the excellent stability of MIL-53Â(Al) allows
highly selective and sensitive detection of Fe<sup>3+</sup> in aqueous
solution. The developed method was applied to the determination of
Fe<sup>3+</sup> in human urine samples with the quantitative spike
recoveries from 98.2% to 106.2%
Intracellular Messenger RNA Triggered Catalytic Hairpin Assembly for Fluorescence Imaging Guided Photothermal Therapy
We
show a theranostic nanoplatform for messenger RNA (mRNA) triggered
enhanced fluorescence imaging guided therapy. Catalytic hairpin assembly
(CHA) and gold nanorods (AuNRs) are employed to fabricate the theranostic
nanoplatform. Two hairpin DNAs and Cy5 labeled duplex DNA are integrated
into the CHA for mRNA triggered fluorescence signal amplification
via hybridization and displacement with mRNA. The AuNRs act both as
the fluorescence quencher and the photothermal therapy (PTT) agent.
The nanoplatform not only enables sensitive and specific imaging of
target mRNA in living cells and good differentiating of the survivin
mRNA expression levels in different cell lines but also offers excellent
photothermal conversion efficiency for PTT. The developed nanoplatform
has great potential for sensitive and specific intracellular mRNA
imaging guided PTT
Room-Temperature Phosphorescent Discrimination of Catechol from Resorcinol and Hydroquinone Based on Sodium Tripolyphosphate Capped Mn-Doped ZnS Quantum Dots
A room-temperature phosphorescence (RTP) strategy was
developed
for direct, additive-free discrimination of catechol from resorcinol
and hydroquinone based on sodium tripolyphosphate capped Mn-doped
ZnS quantum dots (STPP-Mn-ZnS QDs). The RTP response of STPP-Mn-ZnS
QDs to the three isomers was pH-dependent, and the greatest difference
in the RTP response to the isomers was observed at pH 8.0: catechol
enhanced the RTP intensity of the QDs, while resorcinol and hydroquinone
had little effect on the RTP intensity of the QDs. The enhanced RTP
intensity of 1 ÎĽM catechol was not affected by the coexistence
of 30 ÎĽM resorcinol and 50 ÎĽM hydroquinone at pH 8.0.
The detection limit of this RTP method was 53 nM catechol, and the
precision was 3.2% (relative standard deviation) for five replicate
detections of 1 ÎĽM catechol. The discrimination mechanism was
ascribed to the weak bonded ligand of STPP-Mn-ZnS QDs and the different
interaction between the three isomers and STPP-Mn-ZnS QDs. The strong
binding of catechol to Zn resulted in the extraction of Zn from the
surface of STPP-Mn-ZnS QDs and the generation of holes that were trapped
by Mn<sup>2+</sup> to form Mn<sup>3+</sup>. Catechol also promoted
the reduction of Mn<sup>3+</sup> into Mn<sup>2+</sup> excited state,
thus ultimately inducing the enhanced RTP response of STPP-Mn-ZnS
QDs