Phenotype and adhesion assays of <i>KP1_4563</i>.

<p>(A) Hemagglutination assays. Mannose-resistant hemagglutination (MRHA) assays were performed with human erythrocytes. The results are expressed as the minimum bacterial density (CFU/ml) required to cause a visible agglutination reaction. Values represent the mean of three independent experiments, and the error bars represent standard deviation. <i>P</i> values were calculated by one-way ANOVA and Tukey HSD post hoc comparisons. (B) Mannan-binding assay. Mean values and standard deviation of six technical replicates are showed. <i>P</i> values were calculated by one-way ANOVA and LSD post hoc comparisons. (C) Bacterial adhesion assays. Data are the means of measurements made in technical triplicates. Error bars represent the standard deviation. <i>P</i> values were calculated by one-way ANOVA and LSD post hoc comparisons. Significant differences are indicated by * for <i>P</i><0.05 or ** for <i>P</i><0.01.</p

The forest plot for the association between <i>SNP rs12252</i> of <i>IFITM</i>3 and influenza susceptibility in the recessive model (CC vs. CT+TT), subgroup analysis by different severity.

<p>The individual block squares denote the susceptibility for each study published during 2014, with an area proportional to the amount of statistical information in each study. The horizontal line denotes a 95% confidence interval (CI). The pooled estimate and its 95% CI are represented by a diamond.</p

The forest plot for the association between <i>SNP rs12252</i> of <i>IFITM3</i> and influenza susceptibility in the homozygote comparison (CC vs. TT), subgroup analysis by different severity.

<p>The forest plot for the association between <i>SNP rs12252</i> of <i>IFITM3</i> and influenza susceptibility in the homozygote comparison (CC vs. TT), subgroup analysis by different severity.</p

Transcriptional regulation of <i>KP1_4563</i> by CRP.

<p>(A) Quantitative RT-PCR (qRT-PCR). Transcriptional expression of <i>KP1_4563</i> in WT and Kp-Δ<i>crp</i>. The results are expressed as the percentage of WT expression. Data are presented as the mean of at least three technical replicates (mean ± standard deviation). Statistical significance was analyzed by independent samples <i>t</i>- test. Significant difference is indicated by * for <i>P</i><0.05. (B) LacZ fusion assay. The putative promoter region of <i>KP1_4563</i> was cloned into the <i>lacZ</i> transcriptional fusion placZ15 plasmid and then introduced into CCW01 or CCW01Δ<i>crp</i> to determine promoter activity. The results are expressed as β-galactosidase activity (Miller units) in the cellular extracts. Statistical significance was analyzed by independent samples <i>t</i>-test. Significant difference is indicated by * for <i>P</i><0.05. (C) EMSA. The radioactively labeled putative promoter region of <i>KP1_4563</i> was incubated with increasing amounts of purified His–CRP protein with cAMP and was then subjected to 4% (w/v) native polyacrylamide gels electrophoresis. The interaction between His–CRP and the promoter region of <i>KP1_4563</i> formed a DNA–CRP complex, which produced a retarded DNA band with decreased mobility. (D) DNase I footprinting. A labeled coding or non-coding DNA fragment was incubated with increasing amounts of His–CRP (lanes 1, 2, 3, and 4 represent 0, 8.5, 16.9, and 25.4 pmol of purified His–CRP protein, respectively) with cAMP and was then subjected to 8 M urea-6% (w/v) polyacrylamide gels electrophoresis. The footprint region is indicated by vertical bars with positions, and the negative numbers indicate the nucleotide positions upstream of the <i>KP1_4563</i> gene start codon ATG where the A in the ATG start codon refers to position 1.</p

Flow chart of data selection.

<p>Flow chart of data selection.</p

Bacterial strains and plasmids used in this study.

<p>Bacterial strains and plasmids used in this study.</p

Oligonucleotide primers used in this study.

<p>Oligonucleotide primers used in this study.</p

Begg’s funnel plot for publication bias in the recessive model (CC vs. CT+TT) for influenza.

<p>Begg’s funnel plot for publication bias in the recessive model (CC vs. CT+TT) for influenza.</p