The distribution of the TLR4 +896A/G and +1196C/T polymorphisms in gastric cancer.

<p>HWE: Hardy-Weinberg Equilibrium.</p><p>The distribution of the TLR4 +896A/G and +1196C/T polymorphisms in gastric cancer.</p

Association between TLR4 (+896A/G and +1196C/T) Polymorphisms and Gastric Cancer Risk: An Updated Meta-Analysis

<div><p>Background</p><p>Toll-like receptor 4 (TLR4) is a receptor of lipopolysaccharide in the signaling transduction of gastric epithelial cell. It plays a pivotal role in activation of innate immunity and pathogen recognition and thus acts as a modulator in the development and progression of gastric cancer. Growing studies explored the association of polymorphisms in TLR4 with susceptibility to gastric cancer, but the results have remained controversial and conflicting. To investigate the effect of two selected TLR4 (+896A/G and +1196C/T) polymorphisms on gastric cancer, we performed a meta-analysis.</p><p>Methods</p><p>A comprehensive search was conducted to identify all eligible case-control publications investigating the association between TLR4 polymorphisms and gastric cancer risk. Odds ratios (OR) and corresponding 95% confidence intervals (CI) were used to assess such association.</p><p>Results</p><p>Up to March 26 2014, 10 published case-control studies from PubMed and EMBase were available, involving a total of 1888 gastric cancer patients and 3433 control subjects. In the overall meta-analyses, a significantly increased gastric cancer risk was detected in TLR4 +896A/G polymorphism (heterozygous model, AG vs. AA: OR = 1.67, 95% CI, 1.39–2.01; additive model, G vs. A: OR = 1.64, 95% CI, 1.37–1.95) and TLR4 +1196C/T polymorphism (heterozygous model, CT vs. CC: OR = 1.42, 95% CI, 1.11–1.81; additive model, T vs. C: OR = 1.36, 95% CI, 1.08–1.72), similar results were obtained in the subgroup analyses of Caucasian, whereas no associations were detected in any genetic models of non-Caucasian.</p><p>Conclusions</p><p>The overall results suggest that TLR4 polymorphisms (+896A/G and +1196C/T) may be associated with a significantly increased gastric cancer risk in Caucasian.</p></div

Egger's linear regression plots for publication bias test.

<p>A. AG vs. AA; B. G vs. A; C. CT vs. CC; D. T vs. C.</p

Forest plots showing the association of the TLR4 +896A/G polymorphism with H. Pylori infection in gastric cancer patients.

<p>A. AG vs. AA, B. G vs. A.</p

Forest plots showing the association of the TLR4 +896A/G and +1196C/T polymorphisms with risk of gastric cancer.

<p>A. AG vs. AA; B. G vs. A; C. CT vs. CC; D. T vs. C.</p

Characteristics of studies included in the meta-analysis.

<p>HB: hospital based, PB: population based, PCR-RFLP: polymerase chain reaction-restriction fragment length polymorphism.</p><p>Characteristics of studies included in the meta-analysis.</p

Flow diagram of included and excluded studies.

<p>Flow diagram of included and excluded studies.</p

The results of Q-test in all genetic models for TLR4 +896A/G and +1196C/T polymorphisms in gastric cancer.

<p>The results of Q-test in all genetic models for TLR4 +896A/G and +1196C/T polymorphisms in gastric cancer.</p

Begg's funnel plots for publication bias test.

<p>A. AG vs. AA; B. G vs. A; C. CT vs. CC; D. T vs. C.</p

Sensitivity analysis for heterogeneity.

<p>A. AG vs. AA; B. G vs. A; C. CT vs. CC; D. T vs. C.</p