1 research outputs found
Ultrasensitive Ratiometric Homogeneous Electrochemical MicroRNA Biosensing via Target-Triggered Ru(III) Release and Redox Recycling
A new label-free
and enzyme-free ratiometric homogeneous electrochemical
microRNA biosensing platform was constructed via target-triggered
RuĀ(III) release and redox recycling. To design the effective ratiometric
dual-signal strategy, [RuĀ(NH<sub>3</sub>)<sub>6</sub>]<sup>3+</sup> (RuĀ(III)), as one of the electroactive probes, was ingeniously entrapped
in the pores of the positively charged mesoporous silica nanoparticle
(PMSN), and another electroactive probe, [FeĀ(CN)<sub>6</sub>]<sup>3ā</sup> (FeĀ(III)), was selected to facilitate RuĀ(III) redox
recycling due to its distinctly separated reduction potential and
different redox properties. Owing to the liberation of the formed
RNAāssDNA complex from PMSN, the target miRNA triggered the
RuĀ(III) release and was quickly electroreduced to RuĀ(II), and then,
the in-site-generated RuĀ(II) could be chemically oxidized back to
RuĀ(III) by FeĀ(III). Thus, with the release of RuĀ(III) and the consumption
of FeĀ(III), a significant enhancement for the ratio of electroreduction
current [RuĀ(NH<sub>3</sub>)<sub>6</sub>]<sup>3+</sup> over [FeĀ(CN)<sub>6</sub>]<sup>3ā</sup> (<i>I</i><sub>Ru(III)</sub>/<i>I</i><sub>Fe(III)</sub>) value was observed, which
was dependent on the concentration of the target miRNA. Consequently,
a simple, accurate, and ultrasensitive method for the miRNA assay
was readily realized. Furthermore, the limit of detection (LOD) of
our method was down to 33 aM (S/N = 3), comparable or even superior
to other approaches reported in literature. More importantly, it also
exhibited excellent analytical performance in the complex biological
matrix cell lysates. Therefore, this homogeneous biosensing strategy
not only provides an ingenious idea for realizing simple, rapid, reliable,
and ultrasensitive bioassays but also has a great potential to be
adopted as a powerful tool for precision medicine