DataSheet_1_Genome-wide analysis and expression of the aquaporin gene family in Avena sativa L..zip

BackgroundOat (Avena sativa L.) belongs to the early maturity grass subfamily of the Gramineae subfamily oats (Avena) and has excellent characteristics, such as tolerance to barrenness, salt, cold, and drought. Aquaporin (AQP) proteins belong to the major intrinsic protein (MIP) superfamily, are widely involved in plant growth and development, and play an important role in abiotic stress responses. To date, previous studies have not identified or analyzed the AsAQP gene family system, and functional studies of oat AQP genes in response to drought, cold, and salt stress have not been performed.MethodsIn this study, AQP genes (AsAQP) were identified from the oat genome, and various bioinformatics data on the AQP gene family, gene structure, gene replication, promoters and regulatory networks were analyzed. Quantitative real-time PCR technology was used to verify the expression patterns of the AQP gene family in different oat tissues under different abiotic stresses.ResultsIn this study, a total of 45 AQP genes (AsAQP) were identified from the oat reference genome. According to a phylogenetic analysis, 45 AsAQP were divided into 4 subfamilies (PIP, SIP, NIP, and TIP). Among the 45 AsAQP, 23 proteins had interactions, and among these, 5AG0000633.1 had the largest number of interacting proteins. The 20 AsAQP genes were expressed in all tissues, and their expression varied greatly among different tissues and organs. All 20 AsAQP genes responded to salt, drought and cold stress. The NIP subfamily 6Ag0000836.1 gene was significantly upregulated under different abiotic stresses and could be further verified as a key candidate gene.ConclusionThe findings of this study provide a comprehensive list of members and their sequence characteristics of the AsAQP protein family, laying a solid theoretical foundation for further functional analysis of AsAQP in oats. This research also offers valuable reference for the creation of stress-tolerant oat varieties through genetic engineering techniques.</p

Total Syntheses of (−)-Englerins A/B, (+)-Orientalols E/F, and (−)-Oxyphyllol

(−)-Englerin A was synthesized in 20 steps from the commercially available material (<i>R</i>)-(+)-limonene. In addition, (−)-englerin B, (+)-orientalol E/F and (−)-oxyphyllol were obtained from the intermediate in the route. The key steps include a hydroxyl-directing stereoselective and regioselective intramolecular cyclopropanation and a multi-gram-scale stereoselective formal intramolecular [3 + 2] cross cycloaddition ([3 + 2]-IMCC) of a cyclopropane 1,1-diester with a carbonyl. A precursor of 7,10-diastereoisomer of englerins was also obtained

Additional file 1 of Molecular mechanism of vimentin nuclear localization associated with the migration and invasion of daughter cells derived from polyploid giant cancer cells

Additional file 1: Table S1. Detail information of antibodies used in the paper. Table S2. SUMO1-siRNA interfering sequences. Table S3. SUMO2-siRNA interfering sequences. Table S4. SUMO3-siRNA interfering sequences. Table S5. P62-siRNA interfering sequences. Table S6. Vimentin-siRNA interfering sequences. Table S7. CDC42-siRNA interfering sequences. Table S8. CDC42 primer sequences

Highly Efficient Removal of Uranium from Aqueous Solution Using a Magnetic Adsorbent Bearing Phosphine Oxide Ligand: A Combined Experimental and Density Functional Theory Study

It is still a great challenge to develop magnetic adsorbents for the highly efficient entrapment of uranium from aqueous solution. Herein, a novel magnetic adsorbent (denoted as Fe₃O₄/P (AA-MMA-DVP)) bearing phosphine oxide ligand was designed and synthesized via a DPE (1,1-diphenylethylene) method based on DPE as radical controlling agent, showing an excellent adsorption capacity for uranium at pH 4.5 and outstanding selectivity in aqueous media including 14 coexisting ions. The magnetic adsorbent showed a <i>q</i>_max value of 413.2 mg g^–1 at 298 K and pH 4.5, which was higher than that of most of other magnetic adsorbents. The outstanding selectivity (<i>S</i>_u = 95.8%) for uranium was reasonably ascribed to the strong complexation between UO₂²⁺ and PO groups anchored on the polymer skeletion, which was evidenced by experimental results. Furthermore, the magnetic adsorbent could be isolated by magnetic force and be recycled at least five times without significant loss in adsorption capacity. This work provided a convenient synthetic route to develop a novel magnetic adsorbent with high capacity and strong selectivity for the entrapment of uranium from aqueous solution