Supplementary document for Adaptive real-time single-pixel imaging - 6838594.pdf

This document includes the programming details of the proposed method and the illustration of how each of the pattern sequences is arranged to achieve adaptive real-time single-pixel imaging

Data_Sheet_1_Development and validation of Child-Friendly School Environment Questionnaire from Chinese culture.pdf

In the context of building Child-Friendly Cities in China, child-friendly school environments are considered as having a profound impact on children’s development and growth. This study presents the development and validation of the Child-Friendly School Environment Questionnaire for assessing a child-friendly school environment. Utilizing open-ended questions and interviews, an initial questionnaire on the child-friendly school environment was compiled. An exploratory factor analysis of the preliminary test results with 696 primary school children in grades three to six was conducted to refine the questionnaire into a formal 19-item questionnaire. Subsequently, a confirmatory factor analysis was performed to analyze the evaluation results of 807 primary school children in grades three to six. The results indicated that a child-friendly school environment is a multi-dimensional construct encompassing Environment Friendly, Teaching Friendly, Peer Friendly, and Children Participation, with good reliability and validity. The promising outcomes of this study suggest that the Child-Friendly School Environment Questionnaire can be widely used as a powerful evaluation tool for the child-friendly school education practice in the future.</p

Direct and Rapid Quantitative Analysis of Alkyldibenzothiophenes in Deeply Hydrodesulfurized Diesel Fuel by Gas Chromatography Quadrupole Time-of-Flight Mass Spectrometry

Polycyclic aromatic sulfur heterocycles are undesirable compounds in fuel and refined petroleum-based products. The sulfur compounds in diesel are limited strictly to an ultralow concentration according to environmental protection rules. Alkyldibenzothiophenes are recalcitrant species in the hydrodesulfurization process, and speciation of dibenzothiophenes in deeply hydrodesulfurized diesel fuel provides important information to the catalyst and process improvement. However, most of the existing analytical methods are unsuitable for identification of ultralow-sulfur compounds in deeply hydrodesulfurized diesel because of the limits of sensitivity. A new method for direct quantitative analysis of alkyldibenzothiophenes in deeply hydrodesulfurized diesel fuel was developed by gas chromatography quadrupole time-of-flight mass spectrometry, which exhibited good linear response and high precision and sensitivity. The practicability of the method was shown by an extensive qualitative and quantitative analysis of alkyldibenzothiophenes in hydrodesulfurized middle distillates at different sulfur levels

Additional file 1: of Smaller gray matter volume of hippocampus/parahippocampus in elderly people with subthreshold depression: a cross-sectional study

STROBE Statement—Checklist of items that should be included for cross-sectional studies (XLSX 14 kb

Encapsulating Antibiotic and Protein-Stabilized Nanosilver into Sandwich-Structured Electrospun Nanofibrous Scaffolds for <i>MRSA</i>-Infected Wound Treatment

With the increasing prevalence of microbial infections, which results in prolonged inflammation and delayed wound healing, the development of effective and safe antimicrobial wound dressings of multiple properties remains challenging for public health. Despite their various formats, the available developed dressings with limited functions may not fulfill the diverse demands involved in the complex wound healing process. In this study, multifunctional sandwich-structured electrospinning nanofiber membranes (ENMs) were fabricated. According to the structural composition, the obtained ENMs included a hydrophilic inner layer loaded with curcumin and gentamicin sulfate, an antibacterial middle layer consisting of bovine serum albumin stabilized silver oxide nanoparticles, and a hydrophobic outer layer. The prepared sandwich-structured ENMs (SNM) exhibited good biocompatibility and killing efficacy on Escherichia coli, Staphylococcus aureus, and Methicillin-resistant S. aureus (MRSA). In particular, transcriptomic analysis revealed that SNM inactivated MRSA by inhibiting its carbohydrate and energy metabolism and reduced the bacterial resistance by downregulating mecA. In the animal experiment, SNM showed improved wound healing efficiency by reducing the bacterial load and inflammation. Moreover, 16S rDNA sequencing results indicated that SNM treatment may accelerate wound healing without observed influence on the normal skin flora. Therefore, the constructed sandwich-structured ENMs exhibited promising potential as dressings to deal with the infected wound management

Aggregation Interface and Rigid Spots Sustain the Stable Framework of a Thermophilic <i>N</i>‑Demethylase

Enzymes from thermophilic microorganisms usually show high thermostability, which is of great potential in industrial application; to understand the structural logic of these enzymes is helpful for the construction of robust biocatalysts. In this study, based on the crystal structure of an N-demethylaseTrSOXwith outstanding thermostability from Thermomicrobium roseum, substitutions were introduced on the aggregation interface and rigid spots to reduce the aggregation ratio and the rigidity. Four substitutions on the aggregation interfaceV162S, M308S, F170S, and V306Sconsiderably reduced the thermostability and slightly enhanced the catalytic efficiency. In addition, the thermostable framework was considerably disrupted in several multiple P → G substitutions in several local motifs (P129G/P134G, P237G/P259G, and P259G/P276G). These structural fluctuations were in good accordance with whole-structure or partial root-mean-square deviation, radius of gyration H-bonds, and solvent-accessible surface area values in molecular dynamics simulation. Furthermore, these key spots were introduced into an unstable homolog from Bacillus sp., resulting in a dramatical increase in the half-life at 60 °C from <10 to 1440 min. These results could help understand the natural stable framework of thermophilic enzymes, which could be references for the construction of robust enzymes in industrial applications