Gene delivery to in situ veins: Differential effects of adenovirus and adeno-associated viral vectors

AbstractPurpose: Gene transfer offers the potential to modify vein graft biology at the time of surgical implantation. Efficiency of gene delivery, stability of expression, and host responses are critical parameters for candidate vectors. We compared the effects of intraluminal exposure with adenovirus (AD) and adeno-associated virus (AAV) vectors on transgene expression and monocyte adhesion (MA) in treated vein segments. Methods: Adult New Zealand white rabbits (N = 51) were anesthetized, and the jugular veins were cannulated bilaterally. Veins were gently distended with either vector (2·108 to 1·1010 infective particles/mL) or vehicle (control) for 30 minutes, after which venous flow was restored. AD and AAV vectors encoding for the marker genes β-galactosidase (LacZ) and green fluorescent protein (GFP) were used. Vessels were explanted 2 to 40 days postinfection for analysis of gene expression (X-gal staining, reverse transcriptase-polymerase chain reaction), MA, and immunohistochemistry. Ex vivo adhesion assays used 51Cr-labeled THP-1 cells. Statistical significance was tested by using analysis of variance with a P value less than.05. Results: All animals survived, and all treated veins were patent at sacrifice. Intraluminal exposure to AD at a titer of 1·109 resulted in near complete transduction of the endothelium at 2 days, with no detectable expression by day 14. At an equal titer of infectious particles, transgene expression was markedly less for AAV at 2 to 7 days, but improved at 2 weeks and persisted to 40 days. MA was significantly increased 2 days after AD exposure (2.7-fold vs control, *P <.002); AAV treatment had no discernible effect on MA. Conclusion: AD-mediated gene transfer to vein segments resulted in robust, transient gene expression that disappeared after 2 weeks. In comparison, AAV-mediated gene delivery was less efficient, but resulted in delayed onset, persistent expression beyond 30 days. AD exposure induced an early increase in MA to the vein surface that was not seen with AAV treatment. Current generations of both AD and AAV vectors have significant, albeit different, limitations for vascular gene therapy. (J Vasc Surg 2000;31:1149-59.

Fractal aggregation kinetics contributions to thermal conductivity of nano-suspensions in unsteady thermal convection

Nano-suspensions (NS) exhibit unusual thermophysical behaviors once interparticle aggregations and the shear flows are imposed, which occur ubiquitously in applications but remain poorly understood, because existing theories have not paid these attentions but focused mainly on stationary NS. Here we report the critical role of time-dependent fractal aggregation in the unsteady thermal convection of NS systematically. Interestingly, a time ratio λ = t(p)/t(m) (t(p) is the aggregate characteristic time, t(m) the mean convection time) is introduced to characterize the slow and fast aggregations, which affect distinctly the thermal convection process over time. The increase of fractal dimension reduces both momentum and thermal boundary layers, meanwhile extends the time duration for the full development of thermal convection. We find a nonlinear growth relation of the momentum layer, but a linear one of the thermal layer, with the increase of primary volume fraction of nanoparticles for different fractal dimensions. We present two global fractal scaling formulas to describe these two distinct relations properly, respectively. Our theories and methods in this study provide new evidence for understanding shear-flow and anomalous heat transfer of NS associated non-equilibrium aggregation processes by fractal laws, moreover, applications in modern micro-flow technology in nanodevices

Facial Attractiveness of Chinese College Students With Different Sexual Orientation and Sex Roles

Facial attractiveness refers to a positive and joyful emotional experience induced by the face of a target person and the extent to which other people are driven to be close to their wishes. Since the 1970s, face attractiveness has gradually emerged in western psychological research, but most of the studies were confined to heterosexuals. More recently, some scholars have pointed out that sexual orientation may affect the judgment of facial attractiveness of individuals. Based on previous literature, this study proposed to explore the different facial attractiveness of individuals with different sexual orientations and sexual roles. Participants in this study were divided into two types (according to sexual orientation and sexual role) by the Sex Role Inventory for College Students (CSRI). Also, the eye-tracking technique was used to record the path of eye movements, where face images were manipulated by sexual dimorphism clues. The results showed that (1) compared to heterosexual men, homosexual men were significantly more likely to choose masculine faces as more attractive faces in paired faces; (2) male homosexuals are likely to have the feminization bias, and female homosexuals are likely to have the masculinization bias; and (3) the masculine faces are more attractive than feminine faces to participants whose sex role is feminine type and androgynous type

A Novel Equivalent Agglomeration Model for Heat Conduction Enhancement in Nanofluids

We propose a multilevel equivalent agglomeration (MEA) model in which all particles in an irregular cluster are treated as a new particle with equivalent volume, the liquid molecules wrapping the cluster and in the gaps are considered to assemble on the surface of new particle as mixing nanolayer (MNL), the thermal conductivity in MNL is assumed to satisfy exponential distribution. Theoretical predictions for thermal conductivity enhancement are highly in agreement with the classical experimental data. Also, we first try to employ TEM information quantitatively to offer probable reference agglomeration ratio (not necessary a very precise value) to just test rational estimations range by present model. The comparison results indicate the satisfactory priori agglomeration ratio estimations range from renovated model

Evaluation of six satellite-based terrestrial latent heat flux products in the vegetation dominated Haihe river basin of north China

In this study, six satellite-based terrestrial latent heat flux (LE) products were evaluated in the vegetation dominated Haihe River basin of North China. These LE products include Global Land Surface Satellite (GLASS) LE product, FLUXCOM LE product, Penman-Monteith-Leuning V2 (PML_V2) LE product, Global Land Evaporation Amsterdam Model datasets (GLEAM) LE product, Breathing Earth System Simulator (BESS) LE product, and Moderate Resolution Imaging Spectroradiometer (MODIS) (MOD16) LE product. Eddy covariance (EC) data collected from six flux tower sites and water balance method derived evapotranspiration (WBET) were used to evaluate these LE products at site and basin scales. The results indicated that all six LE products were able to capture the seasonal cycle of LE in comparison to EC observations. At site scale, GLASS LE product showed the highest coefficients of determination (R2) (0.58, p 2), followed by FLUXCOM and PML products. At basin scale, the LE estimates from GLASS product provided comparable performance (R2 = 0.79, RMSE = 18.8 mm) against WBET, compared with other LE products. Additionally, there was similar spatiotemporal variability of estimated LE from the six LE products. This study provides a vital basis for choosing LE datasets to assess regional water budget

Non-synchronous Structural and Functional Dynamics During the Coalescence of Two Distinct Soil Bacterial Communities

Soil is a unique environment in which the microbiota is frequently subjected to community coalescence. Additions of organic fertilizer and precipitation of dust induce coalescent events in soil. However, the fates of these communities after coalescence remain uncharted. Thus, to explore the effects of microbiota coalescence, we performed reciprocal inoculation and incubation experiments in microcosms using two distinct soils. The soils were, respectively, collected from a cropland and an industrial site, and the reciprocal inoculation was performed as models for the incursion of highly exotic microbiota into the soil. After incubation under either aerobic or anaerobic conditions for two months, the soils were assayed for their bacterial community structure and denitrification function. According to the 16S rRNA gene sequencing results, the inoculated soil showed a significant shift in bacterial community structure after incubation—particularly in the industrial soil. The structures of the bacterial communities changed following the coalescence but were predicted to have the same functional potential, e.g., nitrogen metabolism, as determined by the quantification of denitrifying genes and nitrogen gas production in the inoculated soil samples, which showed values equivalent those in the original recipient soil samples regardless of inoculum used. The functional prediction based on the known genomes of the taxa that shifted in the incubated sample communities indicates that the high functional overlap and redundancy across bacteria acted as a mechanism that preserved all the metabolic functions in the soil. These findings hint at the mechanisms underlying soil biodiversity maintenance and ecosystem function

The influence of Low Impact Development on rainfall-runoff relationships at catchment scale

With rapid urbanization, Low Impact Development (LID) is promoted as an alternative to Conventional Drainage (CD), seeking a natural solution for current urban water problems. The positive effects of LID were the main theme of recent LID researches, but this project aims to deeply explore the hidden troubles about the extreme peak runoffs influenced by LID city on catchment scale.In this research, the SUPERFLEX conceptual model was adapted to a rural-urban semi-distributed model to simulate the current rainfall-runoff relationship of the catchment where San Antonio city is located in. Besides, the model expressions of 4 representative LID practices (bioretention cells, vegetated swales, green roofs, and permeable pavements) were devised under SUPERFLEX framework. To deal with the prediction uncertainty, three urban development scenarios in 2040 and five LID implementation scenarios were designed for San Antonio city. And their influences on the basin peak runoffs will be quantitively studied.Research result shows that, the urban runoff tended to swing between extreme flood and extreme drought in the reference situation; And next, the infill urban development strategy, which means developing the vacant or undeveloped land within an existing community, was more helpful on peak runoff and total runoff volume control than sprawl urban development strategy with the same population growth; Thirdly, the bioretention cells, vegetated swales, and permeable pavements had similar good performance on peak runoff reduction, which can be mainly ascribe to the stormwater infiltration process. As for the retention of total runoff volume, the bioretention cells, permeable pavements, and green roofs perform better than vegetated swales since the rapid water transportation character of vegetated swales decrease the water residence time for infiltration;The runoff reduction function of LID practices performs effective on the large peaks in dry and normal seasons, but it will be restrained significantly in flood season. According to model result, the rural peak runoffs happened 6.5 to 15.5 hours after the urban peaks. And for 4 LID implementation scenarios in which 15% of urban grey areas is covered by LID practices, the urban peaks are delayed between 0.5 and 2.5 hours. And for the scenario with the LID cover areas as 50% of the urban grey areas, the time lag of urban peaks varies from 0.5 to 6.5 hours. For this scenario, since the obvious time delay of urban peaks, more stack of urban and rural peaks is caused by the time approaching of urban and rural peaks, which causes the increases of two total basin peaks in flood season from 3.57 to 3.65 mm/d and from 6.35 to 6.47 mm/d respectively. In conclusion we may say that the stacking effect of LID implementation on total basin runoff is limited in the case of San Antonio basin, partly due to the fact that only a small part of this basin is urbanized.<br/

The influence of Low Impact Development (LID) on basin runoff in a half-urbanized catchment: A case study in San Antonio, Texas

Low Impact Development (LID) was promoted as an alternative to conventional urban drainage methods. The effects of LID at the site or urban scales have been widely evaluated. This project aims to investigate the impact of LID implementation on basin runoff at a regional scale in a half-urbanized catchment, particularly the overlap of urban and rural sub-flows at peak times. A SUPERFLEX conceptual model framework is adapted as a semi-distributed model to simulate the rainfall-runoff relationship in the catchment for San Antonio, Texas, as a case study. Scenario analyses of both urban development and LID implementation are conducted. Results show that (1) the infill urban development strategy benefits more from runoff control than the sprawl urban development; (2) in non-flood season, permeable pavements, bioretention cells, and vegetated swales decrease peak runoff significantly, and permeable pavements, bioretention cells, and green roofs are good at runoff volume retention; (3) contrary to the general opinion about the peak reduction effect of LID, for a partly urbanized, partly rural basin, the LID implementation delays urban peaks and may cause larger stacking of rural and urban peak runoffs, leading to larger basin peaks under extremely wet conditions.Water Resource

Ground Validation and Error Sources Identification for GPM IMERG Product over the Southeast Coastal Regions of China

The Integrated Multi-satellitE Retrievals for the Global Precipitation Measurement mission (IMERG) has been widely evaluated. However, most of these studies focus on the ultimate merged satellite-gauge precipitation estimate and neglect the valuable intermediate estimates which directly guide the improvement of the IMERG product. This research aims to identify the error sources of the latest IMERG version 6 by evaluating the intermediate and ultimate precipitation estimates, and further examine the influences of regional topography and surface type on these errors. Results show that among six passive microwave (PMW) sensors, the Microwave Humidity Sounder (MHS) has outstanding comprehensive behavior, and Special Sensor Microwave Imager/Sounder (SSMIS) operates advanced at precipitation detection, while the Sounder for Atmospheric Profiling of Humidity in the Intertropics by Radiometry (SAPHIR) has the worst performance. More precipitation events are detected with larger quantitative uncertainty in low-lying places than in highlands, in urban and water body areas than in other places, and more in coastal areas than in inland regions. Infrared (IR) estimate has worse performance than PMW, and the precipitation detectability of IR is more sensitive to the factors of elevation and the distance to the coast, as larger critical successful index (CSI) over lowlands and coastal areas. PMW morphing and the mixing of PMW and IR algorithms partly reverse the conservative feature of the precipitation detection of PMW and IR estimates, resulting in higher probability of detection (POD) and false alert ratio (FAR). Finally, monthly gauge calibration improves most of the statistical indicators and reduces the influence of elevation and surface type factor on these errors