Straightforward Synthesis of <i>N</i>‑Glycan Polymers from Free Glycans via Cyanoxyl Free Radical-Mediated Polymerization

We report a straightforward synthesis of <i>N</i>-glycan polymers from free glycans via glycosylamine intermediates followed by acrylation and polymerization via cyanoxyl-mediated free radical polymerization (CMFRP) in one-pot fashion. No protection and deprotection were used in either glycomonomer or glycopolymer synthesis. A typical synthetic procedure for <i>N</i>-glycan polymers from free monosaccharide and disaccharide, Glc, Gal, Man, GlcNAc, and Lac, was demonstrated. In addition, enzymatic sialylation of the Lac-containing <i>N</i>-glycan polymers and their anti-influenza virus hemagglutination activities were investigated

EIC-MS peaks of all possible caffeoylquinic acids in Re-Du-Ning injection.

<p>EIC-MS peaks of all possible caffeoylquinic acids in Re-Du-Ning injection.</p

MS/MS spectra of compounds 37 (a) and 41 (b) and proposed fragmentation pathways of compound 37.

<p>MS/MS spectra of compounds 37 (a) and 41 (b) and proposed fragmentation pathways of compound 37.</p

MS/MS spectra of compound 35 and proposed fragmentation pathways of compound 35.

<p>MS/MS spectra of compound 35 and proposed fragmentation pathways of compound 35.</p

MS/MS spectra and proposed fragmentation pathways of compounds 39, 40, 42 and 45.

<p>MS/MS spectra and proposed fragmentation pathways of compounds 39, 40, 42 and 45.</p

MS chromatograms of diagnostic ions:

<p>(A) EICs of diagnostic ions 179.0340 and 191.0556 in the high-energy function of MS^E; (B) TIC of RDN in the high-energy function of MS^E; (C) EICs of diagnostic ions 179.0340 and 191.0556 in the low-energy function of MS^E; (D) TIC of RDN in the MS^E low-energy function.</p

Compounds identified in RDN by UPLC-ESI-Q-TOF-MS.

<p>^a<i>Gardenia jasminoides</i> Ellis, <i>Lonicera japonica</i> Thunb. and <i>Artemisia annua</i> are abbreviated as Gj, Lj and Aa, respectively;</p><p>* This compounds were identified by standard compounds;</p><p>Compounds identified in RDN by UPLC-ESI-Q-TOF-MS.</p

Data_Sheet_1_Characterization of volatile organic compounds with anti-atherosclerosis effects in Allium macrostemon Bge. and Allium chinense G. Don by head space solid phase microextraction coupled with gas chromatography tandem mass spectrometry.docx

IntroductionAllium macrostemon Bge. (AMB) and Allium chinense G. Don (ACGD) are both edible Allium vegetables and named officinal Xiebai (or Allii Macrostemonis Bulbus) in East Asia. Their medicinal qualities involve in lipid lowering and anti-atherosclerosis effects. And steroidal saponins, nitrogenous compounds and sulfur compounds are like the beneficial components responsible for medicinal functions. Sulfur compounds are the recognized main components both in the volatile oils of AMB and ACGD. Besides, few researches were reported about their holistic chemical profiles of volatile organic compounds (VOCs) and pharmacodynamic effects.MethodsIn this study, we first investigated the lipid-lowering and anti-atherosclerotic effects of volatile oils derived from AMB and ACGD in ApoE–/– mice with high fat and high cholesterol diets.ResultsThe results showed the volatile oils of AMB and ACGD both could markedly reduce serum levels of TG, TC, and LDL-C (p 0.05). Pathological results displayed they both could obviously improve the morphology of cardiomyocytes and the degree of myocardial fibrosis in model mice. Meanwhile, oil red O staining results also proved they could apparently decrease the lesion areas of plaques in the aortic intima (p DiscussionTaken together, this study was the first analysis of holistic chemical profiles and anti-atherosclerosis effects of AMB and ACGD volatile oils, and would benefit the understanding of effective components in AMB and ACGD.</p

Discovery and LC-MS Characterization of New Crocins in <i>Gardeniae Fructus</i> and Their Neuroprotective Potential

Ten new crocins, neocrocins B–J (<b>1</b>–<b>9</b>) and 13-<i>cis</i>-crocetin-8′-<i>O</i>-β-d-gentiobioside (<b>14</b>), along with 10 known crocins, were isolated from the fruits of Gardenia jasminoides Ellis (<i>Gardeniae Fructus</i>). The structures of these compounds were elucidated by analyzing HRESIMS, UV/vis, and 1D and 2D NMR spectra, and their neuroprotective effects against hydrogen peroxide- and l-glutamic acid-induced SH-SY5Y cell injury were evaluated. The UPLC-Q/TOF-MS chromatogram of a crocin-rich fraction derived from gardenia fruit extracts was established using the obtained crocin compounds as references. Most of the peaks were identified (the total integral area of the identified peaks accounted for 95% of total peak areas), and bioactive crocins were a large portion of this fraction (the areas of peaks from the neuroprotective compounds accounted for 70% of the total)

The amount and frequency of sequences that overlap in the repertoires of IgM and IgG before and after immunization with HBV vaccines in 3 volunteers.

<p>The amount and frequency of sequences that overlap in the repertoires of IgM and IgG before and after immunization with HBV vaccines in 3 volunteers.</p