Table_1_Tinnitus-associated cognitive and psychological impairments: a comprehensive review meta-analysis.XLSX

BackgroundTinnitus is strongly associated with an increased risk of cognitive disabilities. The findings of this research will provide valuable support for future investigations aimed at determining the correlation between tinnitus and the risk of cognitive impairments.ObjectivesWe investigated the potential correlation between tinnitus and the risk of various cognitive impairments, such as dementia, compromised learning attention, anxiety, depression, and insomnia. The study examined this relationship collectively and by categorizing the data based on different age groups.MethodsWe compiled data from case–control studies and cohort studies obtained from reputable databases such as PubMed, Cochrane Library, and Embase. To minimize potential bias, two reviewers independently assessed the selected articles. After extracting the data, we calculated the pooled odds ratios (ORs) using a random-effects model.ResultsSeventeen relevant studies, comprising an adult population, were included in this analysis. Pooled estimated outcomes revealed a strong association between tinnitus and an elevated risk of dementia-compromised learning, auditory attention, anxiety, depression, and poor sleep quality (PConclusionTinnitus has the potential to increase the risk of cognitive impairments. Moreover, geriatric patients aged above 60 shows a higher susceptibility to developing cognitive disabilities compared to their younger counterparts.</p

Image_1_Tinnitus-associated cognitive and psychological impairments: a comprehensive review meta-analysis.JPEG

BackgroundTinnitus is strongly associated with an increased risk of cognitive disabilities. The findings of this research will provide valuable support for future investigations aimed at determining the correlation between tinnitus and the risk of cognitive impairments.ObjectivesWe investigated the potential correlation between tinnitus and the risk of various cognitive impairments, such as dementia, compromised learning attention, anxiety, depression, and insomnia. The study examined this relationship collectively and by categorizing the data based on different age groups.MethodsWe compiled data from case–control studies and cohort studies obtained from reputable databases such as PubMed, Cochrane Library, and Embase. To minimize potential bias, two reviewers independently assessed the selected articles. After extracting the data, we calculated the pooled odds ratios (ORs) using a random-effects model.ResultsSeventeen relevant studies, comprising an adult population, were included in this analysis. Pooled estimated outcomes revealed a strong association between tinnitus and an elevated risk of dementia-compromised learning, auditory attention, anxiety, depression, and poor sleep quality (PConclusionTinnitus has the potential to increase the risk of cognitive impairments. Moreover, geriatric patients aged above 60 shows a higher susceptibility to developing cognitive disabilities compared to their younger counterparts.</p

RNA-Seq reveals differentially expressed genes affecting polyunsaturated fatty acids percentage in the Huangshan Black chicken population

<div><p>Fatty acids metabolic products determine meat quality in chickens. Identifying genes associated with fatty acids composition could provide valuable information for the complex genetic networks of genes with underlying variations in fatty acids synthesis. RNA sequencing (RNA-Seq) was conducted to explore the chicken transcriptome from the thigh muscle tissue of 6 Huangshan Black Chickens with 3 extremely high and low phenotypic values for percentage of polyunsaturated fatty acids (PUFAs). In total, we obtained 41,139,108–44,901,729 uniquely mapped reads, which covered 74.15% of the current annotated transcripts including 18964 mRNA transcripts, across all the six thigh muscle tissue samples. Of these, we revealed 274 differentially expressed genes (DEGs) with a highly significant correlation with polyunsaturated fatty acids percentage between the comparison groups based on the ratio of PUFA/SFA. Gene ontology and pathway analysis indicated that the DEGs were enriched in particular biological processes affecting fatty acids metabolism, biosynthesis of unsaturated fatty acids (USFAs), and cell junction-related pathways. Integrated interpretation of differential gene expression and formerly reported quantitative trait loci (QTL) demonstrated that <i>FADS2</i>, <i>DCN</i>, <i>FRZB</i>, <i>OGN</i>, <i>PRKAG3</i>, <i>LHFP</i>, <i>CHCHD10</i>, <i>CYTL1</i>, <i>FBLN5</i>, and <i>ADGRD1</i> are the most promising candidate genes affecting polyunsaturated fatty acids percentage.</p></div

Volcano plot displaying DEGs within two different comparison groups.

<p>Note: the y-axis shows the mean expression value of log₁₀(q-value), and the x-axis displays the log₂fold change value. The blue dots represent the transcripts did not reach statistical significance (q > 0.05); the red dots represent whose expression levels were significantly different (q < 0.05).</p

Correlations of mRNA expression level of 10 randomly DEGs between high and low polyunsaturated fatty acids percentage using RNA-Seq and qRT-PCR.

<p>Note: the x- and y-axis correspond to the log₂ (ratio of FAH/FAL) measured by RNA-Seq and qRT-PCR, respectively.</p

Top 20 expressed genes in thigh muscle tissue with high polyunsaturated fatty acids percentage compared to low polyunsaturated fatty acids percentage.

<p>Top 20 expressed genes in thigh muscle tissue with high polyunsaturated fatty acids percentage compared to low polyunsaturated fatty acids percentage.</p

Basic sequencing data statistics for each sample.

<p>Basic sequencing data statistics for each sample.</p

Summary of the GO analysis of 20 differentially expressed genes.

<p>Summary of the GO analysis of 20 differentially expressed genes.</p