Ontogenetic Trophic Shifts by <i>Ommastrephes bartramii</i> in the North Pacific Ocean Based on Eye Lens Stable Isotopes

The neon flying squid (Ommastrephes bartramii) plays an important ecological role in the North Pacific. An analysis of stable isotopes in eye lenses was conducted to investigate the inter- and intravariation of the trophic ecology of stocks at the eastern and western North Pacific throughout the life cycle. δ13C and δ15N values gradually increased with ontogenetic growth of the squid, which was associated with geographic migrations and increased the trophic level. For both stocks, from the paralarval to the juvenile stage, the trophic niche breadth increased, which might be the reason that the swimming and feeding ability improved as they entered the juvenile stage. Meanwhile, interactions between different ecosystems led to a greater diversity of food sources; thus, their feeding targets were no longer limited to plankton but shifted toward small fish and other cephalopods. Then, from the juvenile to the subadult–adult stage, the trophic niche breadth decreased, which can be explained by that O. bartramii had a selective preference for certain prey as ontogenetic growth proceeded, and they seemed to focus more on larger prey in the subadult–adult stage. Furthermore, the small amount of overlap between early and later life cycles suggested a significant trophic niche separation among different trophic ecologies and spatial ecologies. This study provides an understanding of diet shifts in neon flying squid in the North Pacific Ocean, primarily including diet shifts during their individual development and differences in trophic variation between the two stocks

Gold-Catalyzed <i>Anti</i>-Markovnikov Oxidation of Au-Allenylidene to Generate Alkylidene Ketene

It remains a long-standing challenge to directly convert alkynes to carboxylic derivatives. Herein, a unexpectedly anti-Markovnikov oxidation of a unique Au-allenylidene pathway instead of a traditional α-oxo gold carbene routine is disclosed for in situ formation and transformation of highly unsaturated alkylidene ketenes, which are subsequently trapped by broad nucleophiles such as alcohols, phenols, water, amines, and sulfoximines to easily access α,β-unsaturated drugs and natural product derivatives by a multicomponent reaction. Based on this scenario, polyacrylate and polyacrylamide are efficiently afforded by corresponding multicomponent polymerization

Phosphatidic acid inhibits SCAB1-mediated F-actin bundling in Arabidopsis

Stomatal closure-associated actin-binding protein 1 (SCAB1) regulates stomatal closure by mediating actin filament reorganization in Arabidopsis thaliana. Our previous study showed that phosphatidylinositol 3-phosphate (PI3P) binds to SCAB1 and inhibits its oligomerization, thereby inhibiting its activity on F-actin in guard cells during stomatal closure. In this study, we show that another phospholipid, phosphatidic acid (PA), also binds to SCAB1 and inhibits its actin-bundling activity but not its actin-binding activity. F-actin bundling was promoted in vivo by treating Col-0 seedlings with n-butanol, a suppressor of PA production, but this effect was absent in the scab1 mutant. These results indicate that the signaling molecule PA is involved in the modulation of SCAB1 activity in F-actin reorganization

A Perovskite-based multifunctional cathode with simultaneous supplementation of substrates and electrons for enhanced microbial electrosynthesis of organics

Microbial electrosynthesis (MES) is an electricity-driven technology for the microbial reduction of CO2 to organic commodities. However, the limited solubility of CO2 in a solution and the inefficiency electron transfer make it impossible for microorganisms to obtain an efficient surface for catalytic interaction, thus resulting in the low efficiency of MES. To address this, we introduce a multifunctional perovskite-based cathode material (Pr0.5(Ba0.5Sr0.5)0.5Co0.8Fe0.2O3-δ-carbon felt (Pr0.5BSCF-CF), which provides a simultaneously significant increase in CO2 absorption and hydrogen production. As a result, the volumetric acetate production rate of MES obtained by Pr0.5BSCF-CF is 0.24 ± 0.01 g L-1 d-1, and it achieves a maximum acetate titer of 13.74 ± 0.20 g L-1 within 70 days. An adequate supply of CO2 and H2 also provides a sufficient amount of substrates and energy for the self-replication of the biocatalysts in the MES reactor. This effect not only increases the amount of biocatalysts but also optimizes the functions of the biocatalysts; the above benefits further improve the production efficiency of the MES system. This strategy demonstrates that the development of perovskite-based multifunctional cathodes with a simultaneous supplementation of substrates and electrons is a promising approach towards improving MES efficiency

Regulation of bacteria population behaviors by AI-2 “consumer cells” and “supplier cells”

Abstract Background Autoinducer-2 (AI-2) is a universal signal molecule and enables an individual bacteria to communicate with each other and ultimately control behaviors of the population. Harnessing the character of AI-2, two kinds of AI-2 “controller cells” (“consumer cells” and “supplier cells”) were designed to “reprogram” the behaviors of entire population. Results For the consumer cells, genes associated with the uptake and processing of AI-2, which includes LsrACDB, LsrFG, LsrK, were overexpressed in varying combinations. Four consumer cell strains were constructed: Escherichia coli MG1655 pLsrACDB (NK-C1), MG1655 pLsrACDBK (NK-C2), MG1655 pLsrACDBFG (NK-C3) and MG1655 pLsrACDBFGK (NK-C4). The key enzymes responsible for production of AI-2, LuxS and Mtn, were also overexpressed, yielding strains MG1655 pLuxS (NK-SU1), and MG1655 pLuxS-Mtn (NK-SU2). All the consumer cells could decrease the environmental AI-2 concentration. NK-C2 and NK-C4 were most effective in AI-2 uptake and inhibited biofilm formation. While suppliers can increase the environmental AI-2 concentration and NK-SU2 was most effective in supplying AI-2 and facilitated biofilm formation. Further, reporter strain, MG1655 pLGFP was constructed. The expression of green fluorescent protein (GFP) in reporter cells was initiated and guided by AI-2. Mixture of consumer cells and reporter cells suggest that consumer cells can decrease the AI-2 concentration. And the supplier cells were co-cultured with reporter cells, indicating that supplier cells can provide more AI-2 compared to the control. Conclusions The consumer cells and supplier cells could be used to regulate environmental AI-2 concentration and the biofilm formation. They can also modulate the AI-2 concentration when they were co-cultured with reporter cells. It can be envisioned that this system will become useful tools in synthetic biology and researching new antimicrobials

Ligand-Controlled Remarkable Regio- and Stereodivergence in Intermolecular Hydrosilylation of Internal Alkynes: Experimental and Theoretical Studies

The first highly efficient ligand-controlled regio- and stereodivergent intermolecular hydrosilylations of internal alkynes have been disclosed. Cationic ruthenium complexes [Cp{*}Ru(MeCN)(3)](+) and [CpRu(MeCN)(3)](+) have been demonstrated to catalyze intermolecular hydrosilylations of silyl alkynes to form a range of vinyldisilanes with excellent but opposite regio- and stereoselectivity, with the former being alpha anti addition and the latter beta syn addition. The use of a silyl masking group not only provides sufficient steric bulk for high selectivity but also leads to versatile product derivatizations toward a variety of useful building blocks. DFT calculations suggest that the reactions proceed by a mechanism that involves oxidative hydrometalation, isomerization, and reductive silyl migration. The energetics of the transition states and intermediates varies dramatically with the catalyst ligand (Cp{*} and Cp). Theoretical studies combined with experimental evidence confirm that steric effect plays a critical role in governing the regio- and stereoselectivity, and the interplay between the substituent in the alkyne (e.g., silyl group) and the ligand ultimately determines the observed remarkable regio- and stereodivergence

Introducing Two Fixed Platforms in the Yellow Sea and East China Sea Supporting Long-Term Satellite Ocean Color Validation: Preliminary Data and Results

Following the Aerosol Robotic Network-Ocean Color (AERONET-OC) network scheme and instrument deployment protocols, two fixed platforms (Muping and Dong’ou) in the Yellow Sea and East China Sea were implemented with the support of the China National Satellite Ocean Application Service. Optical radiometry instruments were established at the two sites to autonomously determine remote sensing reflectance (Rrs) and aerosol optical depth (AOD). Details about location selection, platform design, instrument deployment, and the associated data processing procedure are reported in this study. Rrs and AOD measured by independent instruments at the Muping site were compared and results showed that they were consistent, with a median relative percentage difference (MRPD) Rrs. The spectral feature and temporal pattern of Rrs and AOD at the two sites were examined and compared with data from 14 AERONET-OC sites. Rrs and AOD data measured at the two sites were used to evaluate ocean color operational products of MODIS/Aqua (MODISA), OLCI/Sentinel-3A (OLCI-3A), and OLCI/Sentinel-3B (OLCI-3B). Comparison showed that the three satellite sensor-derived Rrs agreed well with in situ measurements, with an MRPD < 25% for MODISA, <30% for OLCI-3A, and <40% for OLCI-3B, respectively. Large uncertainties were observed in the blue bands for the three satellite sensors, particularly for OLCI-3B at 400 nm. AOD at 865 nm derived from the three satellite sensors also agreed well with in situ measurements, with an MRPD of 28.1% for MODISA, 30.6% for OLCI-3A, and 39.9% for OLCI-3B. Two commonly used atmospheric correction (AC) processors, the ACOLITE and SeaDAS, were also evaluated using in situ measurements at two sites and 20 m-resolution MSI/Sentinel-2A data. Close agreements were achieved for both AC processors, while the SeaDAS performed slightly better than ACOLITE. The optimal band selection in the AC models embedded in two AC processors was a combination of one near-infrared and one short-wave infrared band such as 865 and 1609 nm, shedding light on MSI data applications in the aquatic environment