63 research outputs found
The Organic Amendment Improve the Yield and Quality of Vegetable
Using biotechnology, we can change agricultural wastes into high‐quality organic fertilizers, which leads us in the direction of the development in modern agriculture and act as substitute to the chemical fertilizers. In this chapter, five types of technologies of organic amendment are elaborated. Each method can be selected based on the specific circumstance. The effects of the technology in the production are introduced and the principles of the technologies are explained in a simple manner
Dihydroflavonol 4-Reductase Genes from Freesia hybrida Play Important and Partially Overlapping Roles in the Biosynthesis of Flavonoids
Characterization and Gene Mapping of an Open-Glume <i>Oryza sativa</i> L. Mutant
Floral organ development determines agricultural productivity by affecting seed development, seed quality, and final yield. In this study, we described the novel ogl mutant in rice (Oryza sativa L.), which is characterized by an open-glume phenotype, increased pistil number, reduced stamen number, decreased seed setting rate, and smaller rice grains. Genetic analysis showed that the open-glume phenotype might be controlled by a recessive qualitative trait locus. Employing bulked segregant analysis (BSA), one candidate region was identified on rice chromosome 1. The glume opening phenotype cosegregated with SNP (Chr1:1522703), which was located at the start codon of one transcript of OsJAG, resulting in partial loss of OsJAG function. cDNA analysis revealed that OsJAG encodes two transcript variants. Compared to normal plants, the expression of OsJAG.1 was upregulated in open-glume plants. When investigating the glume phenotype, we found that the expression of genes related to floral development changed greatly in open-glume plants. Taken together, this work increases our understanding of the developmental role of OsJAG in rice floral development
A 1 × 2 Two-Dimensional Slanted Grating Based on Double-Layer Cylindrical Structure
Diffraction gratings play an increasingly important role in various planar optical systems, such as near-eye display systems for virtual reality (VR) and augmented reality (AR). The slanted gratings have more advantages than other elements. A 1 × 2 transmission two-dimensional (2D) slanted grating based on a double-layer cylindrical structure was proposed in this paper. In the initial phase of this study, this kind of grating was proposed and designed. We used rigorous coupled-wave analysis (RCWA) and simulated annealing algorithm (SA) to optimize the grating parameters. The effects of the grating geometric parameters on the diffraction efficiency were investigated using rigorous coupled-wave analysis (RCWA). The simulated annealing algorithm (SA) optimization results show that the diffraction efficiency of the (0, −1) and (−1, 0) order exceed 35% under normal incidence in the range of 429–468 nm wavelength for TE and TM polarization. Meanwhile, the total diffraction efficiency can reach up to 78%. In the last section, we discuss the tolerances for the grating parameters to ensure high quality manufacturing processes. The total effective efficiency is greater than 75% when the MgF2 thickness is from 300 nm to 350 nm and the SiO2 thickness is from 525 nm to 550 nm. Moreover, the grating period has a 53 nm fabrication tolerance, and the slanted angle has a 8.8-degree fabrication tolerance. The relatively large tolerances ensure that it is easy to fabricate the two-dimensional slanted grating and to achieve the targeted objectives. The proposed 2D slanted grating can be applied to 2D exit pupil expansion, which is of great importance in AR/VR applications
Identification of Genes Associated with Morphology in Aspergillus niger by Using Suppression Subtractive Hybridization
The morphology of citric acid production strains of Aspergillus niger is sensitive to a variety of factors, including the concentration of manganese (Mn(2+)). Upon increasing the Mn(2+) concentration in A. niger (ATCC 11414) cultures to 14 ppb or higher, the morphology switches from pelleted to filamentous, accompanied by a rapid decline in citric acid production. The molecular mechanisms through which Mn(2+) exerts effects on morphology and citric acid production in A. niger cultures have not been well defined, but our use of suppression subtractive hybridization has identified 22 genes responsive to Mn(2+). Fifteen genes were differentially expressed when A. niger was grown in media containing 1,000 ppb of Mn(2+) (filamentous form), and seven genes were expressed in 10 ppb of Mn(2+) (pelleted form). Of the 15 filament-associated genes, seven are novel and eight share 47 to 100% identity with genes from other organisms. Five of the pellet-associated genes are novel, and the other two genes encode a pepsin-type protease and polyubiquitin. All 10 genes with deduced functions are either involved in amino acid metabolism-protein catabolism or cell regulatory processes. Northern blot analysis showed that the transcripts of all 22 genes were rapidly enhanced or suppressed by Mn(2+). Steady-state mRNA levels of six selected filament-associated genes remained high during 5 days of culture in a filamentous state and remained low under pelleted growth conditions. The opposite behavior was observed for four selected pellet-associated genes. The full-length cDNA of the filament-associated clone, Brsa-25, was isolated. Antisense expression of Brsa-25 permitted pelleted growth and increased citrate production at concentrations of Mn(2+) that were higher than the parent strain could tolerate. These results suggest the involvement of the newly isolated genes in the regulation of A. niger morphology
Fuzzy evaluation of ecological vulnerability based on the SRP-SES method and analysis of multiple decision-making attitudes based on OWA operators: A case of Fujian Province, China
Evaluation of the ecological vulnerability facilitates ecological protection and management. We presented a well-organized and comprehensive evaluation system and analysis method of ecological vulnerability. We used Fujian Province, China, as a case study and validated the reliability of evaluation results. The details are as follows. Based on the framework of sensitivity-resilience-pressure (SRP) coupled with the “social-environmental” system (SES), this study selected 23 indicators and determined their weights by a combination of subjective and objective weighting methods to systematically construct the ecological vulnerability evaluation system. By establishing a fuzzy evaluation model, the indicator data were fuzzily mapped into evaluation scores for the calculation of the ecological vulnerability index (EVI). Following the calculation results, the spatial clustering analysis based on K-means, driving factor analysis based on GeoDetector and spatial autocorrelation analysis were conducted. Finally, an ecological vulnerability analysis under multiple decision-making attitudes was conducted based on the ordered weighted average (OWA) operators. The results were as follows. (1) The ecological vulnerability of Fujian Province showed a trend of higher in the east and lower in the west, and there is an obvious spatial aggregation effect. The high-high aggregation areas mainly distributed in the eastern coastal areas of Fujian Province, and the low-low aggregation areas mainly distributed in the western and central areas. (2) Landscape pattern sensitivity had the highest degree of explanation for the EVI. The main driving factors of the EVI in coastal cities were different from those of the EVI in inland cities. (3) We divided the study area into four categories: high-quality development areas, transformational development areas, ecological-economic synergy areas and ecological function areas. (4) With an increase in the decision-making risk coefficient, the ecological vulnerability of Fujian Province gradually increased. The results of the study provide important guidance for ecological protection and sustainable development in Fujian Province. Moreover, the study framework has reference value for related studies of regional ecological vulnerability evaluation
Simulation Research on Energy Evolution and Supply Law of Rock–Coal System under the Influence of Stiffness
The energy supply effect caused by the stiffness difference between roofs and sidewalls is an important factor that induces strain coal bursts. In order to quantitatively reveal the energy supply mechanism of strain coal bursts, this paper first establishes a coal burst energy model of the rock–coal system and proposes the calculation formula of coal burst kinetic energy considering supply energy and the stiffness ratio of rock to coal. Then the whole energy evolution law of the rock–coal system with different stiffness ratios is researched by using the numerical simulation method, and the whole process is divided into three stages. With the decrease in the stiffness ratio, the elastic strain energy of the coal changes little, while its kinetic energy is negatively correlated with the stiffness ratio in a power function. Meanwhile, the elastic strain energy and kinetic energy of the rock have power function relations with the stiffness ratio, too. When the rock–coal system is fractured, the kinetic energy of the coal comes from the release of elastic strain energy from the coal and the energy supplied from the rock. The energy supply rate is between 22% and 35% when the stiffness ratio changes from 3.0 to 0.5, and they show a linear relationship, while the supplied energy has a negative power function relationship with the stiffness ratio
A 1 × 2 Two-Dimensional Slanted Grating Based on Double-Layer Cylindrical Structure
Diffraction gratings play an increasingly important role in various planar optical systems, such as near-eye display systems for virtual reality (VR) and augmented reality (AR). The slanted gratings have more advantages than other elements. A 1 × 2 transmission two-dimensional (2D) slanted grating based on a double-layer cylindrical structure was proposed in this paper. In the initial phase of this study, this kind of grating was proposed and designed. We used rigorous coupled-wave analysis (RCWA) and simulated annealing algorithm (SA) to optimize the grating parameters. The effects of the grating geometric parameters on the diffraction efficiency were investigated using rigorous coupled-wave analysis (RCWA). The simulated annealing algorithm (SA) optimization results show that the diffraction efficiency of the (0, −1) and (−1, 0) order exceed 35% under normal incidence in the range of 429–468 nm wavelength for TE and TM polarization. Meanwhile, the total diffraction efficiency can reach up to 78%. In the last section, we discuss the tolerances for the grating parameters to ensure high quality manufacturing processes. The total effective efficiency is greater than 75% when the MgF2 thickness is from 300 nm to 350 nm and the SiO2 thickness is from 525 nm to 550 nm. Moreover, the grating period has a 53 nm fabrication tolerance, and the slanted angle has a 8.8-degree fabrication tolerance. The relatively large tolerances ensure that it is easy to fabricate the two-dimensional slanted grating and to achieve the targeted objectives. The proposed 2D slanted grating can be applied to 2D exit pupil expansion, which is of great importance in AR/VR applications
Sodium Alginate as a Novel Cryoprotective Agent for Cryopreservation of Endothelial Cells in a Closed Polytetrafluoroethylene Loop
Acute myocardial infarction in a 39-year-old lactating woman
A 39-year-old Chinese woman presented to the department of emergency with chest pain radiating to the left shoulder and arm accompanied with sweating and nausea for 4 h. Her blood pressure was 120/70 mmHg symmetrically over both arms and her pulse was 70 beats per minute. Her physical examination was normal. Electrocardiography showed sinus rhythm with ST-segment elevation in leads II and aVF. Laboratory data revealed cardiac troponin I of 3.0 ng/ml (relative index < 0.02 ng/ml), creatine kinase MB of 106.0 ng/ml (relative index < 7.0 ng/ml), and myoglobin of 117.0 ng/ml (relative index < 112.0 ng/ml)
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