Differential Impacts of Soybean and Fish Oils on Hepatocyte Lipid Droplet Accumulation and Endoplasmic Reticulum Stress in Primary Rabbit Hepatocytes

Parenteral nutrition-associated liver disease (PNALD) is a severe ailment associated with long-term parenteral nutrition. Soybean oil-based lipid emulsions (SOLE) are thought to promote PNALD development, whereas fish oil-based lipid emulsions (FOLE) are thought to protect against PNALD. This study aimed to investigate the effects of SOLE and FOLE on primary rabbit hepatocytes. The results reveal that SOLE caused significant endoplasmic reticulum (ER) and mitochondrial damage, ultimately resulting in lipid droplets accumulation and ER stress. While these deleterious events induce hepatocyte injury, FOLE at high doses cause only minor ER and mitochondrial damage, which has no effect on hepatic function. SOLE also significantly upregulated glucose-regulated protein 94 mRNA and protein expression. These data indicate that SOLE, but not FOLE, damage the ER and mitochondria, resulting in lipid droplets accumulation and ER stress and, finally, hepatocyte injury. This likely contributes to the differential impacts of SOLE and FOLE on PNALD development and progression

Genome-Wide Identification, Sequence Variation, and Expression of the Glycerol-3-Phosphate Acyltransferase (GPAT) Gene Family in Gossypium

Cotton is an economically important crop grown for natural fiber and seed oil production. Cottonseed oil ranks third after soybean oil and colza oil in terms of edible oilseed tonnage worldwide. Glycerol-3-phosphate acyltransferase (GPAT) genes encode enzymes involved in triacylglycerol biosynthesis in plants. In the present study, 85 predicted GPAT genes were identified from the published genome data in Gossypium. Among them, 14, 16, 28, and 27 GPAT homologs were identified in G. raimondii, G. arboreum, G. hirsutum, and G. barbadense, respectively. Phylogenetic analysis revealed that a total of 108 GPAT genes from cotton, Arabidopsis and cacao could be classified into three groups. Furthermore, through comparison, the gene structure analyses indicated that GPAT genes from the same group were highly conserved between Arabidopsis and cotton. Segmental duplication could be the major driver for GPAT gene family expansion in the four cotton species above. Expression patterns of GhGPAT genes were diverse in different tissues. Most GhGPAT genes were induced or suppressed after salt or cold stress in Upland cotton. Eight GhGPAT genes were co-localized with oil and protein quantitative trait locus (QTL) regions. Thirty-two single nucleotide polymorphisms (SNPs) were detected from 12 GhGPAT genes, sixteen of which in nine GhGPAT genes were classified as synonymous, and sixteen SNPs in ten GhGPAT genes non-synonymous. Two SNP markers of the GhGPAT16 and GhGPAT26 genes were significantly correlated with cotton oil content in one of the three field tests. This study shed lights on the molecular evolutionary properties of GPAT genes in cotton, and provided reference for improvement of cotton response to abiotic stress and the genetic improvement of cotton oil content

Genome-Scale Analysis of the WRI-Like Family in Gossypium and Functional Characterization of GhWRI1a Controlling Triacylglycerol Content

Cotton (Gossypium spp.) is the most important natural fiber crop and the source of cottonseed oil, a basic by-product after ginning. AtWRI1 and its orthologs in several other crop species have been previously used to increase triacylglycerols in seeds and vegetative tissues. In the present study, we identified 22, 17, 9, and 11 WRI-like genes in G. hirsutum, G. barbadense, G. arboreum, and G. raimondii, respectively. This gene family was divided into four subgroups, and a more WRI2-like subfamily was identified compared with dicotyledonous Arabidopsis. An analysis of chromosomal distributions revealed that the 22 GhWRI genes were distributed on eight At and eight Dt subgenome chromosomes. Moreover, GhWRI1a was highly expressed in ovules 20–35 days after anthesis and was selected for further functional analysis. Ectopic expression of GhWRI1a rescued the seed phenotype of a wri1-7 mutant and increased the oil content of Arabidopsis seeds. Our comprehensive genome-wide analysis of the cotton WRI-like gene family lays a solid foundation for further studies

Effect of the structure of Ni nanoparticles on the electrocatalytic activity of Ni@Pd/C for formic acid oxidation

Ni@Pd/C catalysts were synthesized, using Ni/C with different crystalline structures prepared with various ligands. A series of characterizations were performed by transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy. The results indicated the electrocatalysts with amorphous/crystalline (denoted as Nia and Nic) Ni structures decorated with Pd. The formic acid electrocatalytic oxidation results showed that the peak current of Nia@Pd/C was about 1.2 times higher than that of Nic@Pd/C. The good electrochemical performance and stability of Pd-modified amorphous Ni substrate reveals that the core structure plays an important role in the electrocatalytic activity and the change of the structure can improve the activity and stability of electrocatalysts.Web of Scienc

Tillage and crop straw methods affect energy use efficiency, economics and greenhouse gas emissions in rainfed winter wheat field of Loess Plateau in China

Data from a field experiment conducted in China's Loess Plateau (2013–2015) were used to determine the energy balance of winter wheat (Triticum aestivum L.) as affected by tillage and straw treatments. Tillage treatments included chisel plow, no tillage, and mouldboard plow. Crop straw levels included straw returning and straw removed. The energy balance was evaluated by comparing the following parameters: net energy, energy profitability, energy use efficiency, and energy intensity. The yield parameters were significantly influenced by the tillage treatments and revealed that the chisel plow entailed fewer field operations and lower energy requirements with a higher yield than mouldboard plowing tillage. The highest proportion of energy input came from a nitrogen fertiliser, followed by diesel fuel. The total energy input applied per hectare increased with an increase in the tillage intensity, and the lowest energy input was required for the no tillage case with the straw returning treatment, and the highest for the case of mouldboard plow with the straw returning treatment. The lowest average energy intensity was recorded for the no tillage case, followed by the case of chisel plow tillage in both cropping seasons. Moreover, in the case of mouldboard plough tillage, the maximum energy intensity was recorded in both cropping seasons. In the cases of the chisel plow tillage and the no tillage, we observed the maximum energy gain, while in the no tillage case, we observed the maximum energy use efficiency. The net return and the benefit/cost ratio were higher in the case of straw returning than those in the case of no straw treatment. We concluded that no tillage and chisel plow tillage with straw returning could improve the energy use efficiency and the benefit/cost ratio of winter wheat production systems

A Maximum Efficiency-86% Hybrid Power Modulator for 5G New Radio(NR) Applications

A hybrid power modulator is presented for the radio frequency (RF) power amplifiers of 5th generation mobile communication technology (5G) new radio (NR) applications. A hybrid power modulator utilizing a two-level switching converter and a broadband and high-efficiency linear amplifier is presented. A further improvement in the efficiency of the circuit is achieved by using an optimized supply voltage of the two-level switching converters of 4.5 V. In this way, the overall efficiency is improved by more than 5% compared to using a 5 V supply voltage. The linear amplifier consists of four stages. In order to improve bandwidth and circuit stability, a compensation circuit is added to the linear amplifier that eliminates the poles of the main amplifier by introducing additional zeros, indirectly pushing the pole distribution out of the bandwidth. Using this approach, the linear amplifier achieves a 3-dB bandwidth of 180 MHz and an efficiency of 51%. The hybrid power modulator achieves a maximum output power of 2.4 W and an efficiency of 86% when tracking a 100 MHz 5G-NR signal under a 4 Ω load in a 180 nm CMOS package

Three-Level Hybrid Envelope Tracking Supply Modulator with High-Bandwidth Wide-Output-Swing

Envelope tracking is a dynamic supply modulation technique, which is mainly used to improve the efficiency of radio frequency power amplifier. For 5G NR mobile devices, this paper presents an envelope tracking supply modulator which is composed of a three-level switching converter and linear amplifier (LA) in parallel. The hysteresis control method is adopted in the supply modulator to improve the bandwidth of the switching converter and the loop response speed. At the same time, a rail-to-rail input-output class AB linear amplifier is designed to achieve a wide output swing and a super source follower is proposed to reduce the output impedance of LA. Designed with the 180 nm CMOS technology, the supply modulator operated at 5G NR 100 MHz envelope signal. The output power of the modulator reaches 1.5 W for a 6 Ω load resistor and the output swing is 4.3 V at 5.0 V supply, and the maximum efficiency reaches 85%

Design of 2–16 GHz Non-Uniform Distributed GaN HEMT MMIC Power Amplifier with Harmonic Suppression Network

In this paper, an ultra-wideband (UWB) power amplifier (PA) on a 0.25 μm gallium-nitride (GaN) on silicon carbide (SiC) high-electron-mobility transistor (HEMT) process, operating in Ku-band, is presented. The broadband PA design is based on the four-stage non-uniform distributed amplifier structure. In order to improve the efficiency of the PA, a harmonic suppression network is added at the output of the drain artificial transmission line. At the same time, a capacitor is connected in series at the input of the gate, which is used to compensate for the phase offset of the gate and increase the cut-off frequency of the PA. The final gate width of the first stage is 0.56 μm, and the other three-stage gate widths are all 0.32 μm. Over the frequency range of 2–16 GHz, the simulated results of this NDPA exhibit a power-added efficiency (PAE) of 16.6–27%, a saturated continuous wave (CW) output power of 35–37 dBm, a small signal gain of 9.1–11.6 dB, and output return losses of 5–15 dB

Design of 2–16 GHz Non-Uniform Distributed GaN HEMT MMIC Power Amplifier with Harmonic Suppression Network

In this paper, an ultra-wideband (UWB) power amplifier (PA) on a 0.25 μm gallium-nitride (GaN) on silicon carbide (SiC) high-electron-mobility transistor (HEMT) process, operating in Ku-band, is presented. The broadband PA design is based on the four-stage non-uniform distributed amplifier structure. In order to improve the efficiency of the PA, a harmonic suppression network is added at the output of the drain artificial transmission line. At the same time, a capacitor is connected in series at the input of the gate, which is used to compensate for the phase offset of the gate and increase the cut-off frequency of the PA. The final gate width of the first stage is 0.56 μm, and the other three-stage gate widths are all 0.32 μm. Over the frequency range of 2–16 GHz, the simulated results of this NDPA exhibit a power-added efficiency (PAE) of 16.6–27%, a saturated continuous wave (CW) output power of 35–37 dBm, a small signal gain of 9.1–11.6 dB, and output return losses of 5–15 dB