P450scc protein expression in preeclampsia group was significantly higher than normal control.

<p>Left panel: representative western blot result of the placenta samples, Right panel: statistic of western blot result from 53 normal samples and 59 preeclampsia samples (p<0.05).</p

Enhanced apoptosis in CYP11A overexpressed HTR-8/SVneo cells.

<p>(A) TUNEL staining shows increased positive signals in CYP11A overexpressed HTR-8/SVneo cells compared to vector control when cultured in hypoxia condition. (B) Western blot result showed increased cleaved caspase-3 expression in CYP11A overexpressed HTR-8/SVneo compared to vector control cells. The image shown is representative result of two independant experiments.</p

of CYP 11A mRNA in normal pregnancy group, severe pre-eclampsia group compared with the relative optical density of expression in mild preeclampsia.

<p>Normal pregnancy group with severe preeclampsia group: p = 0.015,95% CI (−0.5388, −0.0595).</p><p>Normal pregnancy group compared with mild preeclampsia: p = 0.241,95% CI (−0.6510, 0.1565).</p><p>Mild preeclampsia and severe preeclampsia group: p = 0.727,95% CI (− 0.4656, 0.3259).</p

p450scc protein expression multiple regression analysis result.

a<p>Dependent Variable: lgp450db.</p

Linear multiple regression result of P450scc protein expression and clinical parameters.

<p>(A) CYP11A expression negatively correlated to INR (P = 0.095). (B) CYP11A expression positively correlated to Plt (P = 0.018). (C) CYP11A expression positively correlated to ALT (P = 0.02).</p

p450scc protein expression in normal pregnancy and preeclampsia group relative optical density.

<p>t = 3.528; 95% CI (0.9424,3.3623).</p

Immunohistochemistry staining of P450scc in placenta tissue.

<p>(A) Upper panel: Visible P450scc positive expression located in the cytoplasm of syncytiotrophoblast of placenta (arrow). Left: negative control without primary antibody. Right: P450scc (CYP11A) staining; Manification 600x; scale bar = 40 uM. Down panel: the higher manification pictures of the insert part of the above figures (B) Western blot shows that there is no significant different of CYP11A expression between first and third trimester placentas.</p

Mechanical-stretch stimulates cell cycling of C2C12 cells.

<p>(<b>A</b>) Phase contrast microscopical analysis of C2C12 myoblasts stretched or not during 2d. Cell number was higher in stretched groups (10%, 0.25Hz) than in unstretched controls. (<b>B</b>) Flow cytometric analysis of the percentage of cells in G₁, S, or G₂/M phases of the cell cycle. (<b>C</b>) Statistical analysis of the relative DNA proliferation index (DPI) of stretched (10%, 0.25Hz) or unstretched C2C12 myoblasts. Values represent mean ± SD (<i>n</i>=3 per group). <i>p</i> values were determined by independent-sample <i>t</i> tests (**<i>p</i><0.001, *<i>p</i><0.05).</p

Table_1_Short interpregnancy interval can lead to adverse pregnancy outcomes: A meta-analysis.pdf

BackgroundThe evidence of some previous papers was insufficient in studying the causal association between interpregnancy interval (IPI) and adverse pregnancy outcomes. In addition, more literature have been updated worldwide during the last 10 years.MethodsEnglish and Chinese articles published from January 1980 to August 2021 in the databases of PubMed, Cochrane Library, Ovid, Embase, China Biology Medicine disc (CBM), and China National Knowledge Infrastructure (CNKI) were searched. Then following the inclusion and exclusion criteria, we screened the articles. Utilizing the Newcastle–Ottawa Scale (NOS), we evaluated the quality of the included articles. The literature information extraction table was set up in Excel, and the meta-analysis was performed with Stata 16.0 software (Texas, USA).ResultsA total of 41 articles were included in the meta-analysis, and NOS scores were four to eight. The short IPI after delivery was the risk factor of preterm birth (pooled odds ratio 1.49, 95% confidence interval 1.42–1.57), very preterm birth (pooled OR: 1.82, 95% CI: 1.55–2.14), low birth weight (pooled OR: 1.33, 95% CI: 1.24–1.43), and small for gestational age (pooled OR: 1.14, 95% CI: 1.07–1.21), offspring death (pooled OR: 1.60, 95% CI: 1.51–1.69), NICU (pooled OR: 1.26, 95% CI: 1.01–1.57), and congenital abnormality (pooled OR: 1.10, 95% CI: 1.05–1.16), while was not the risk factor of gestational hypertension (pooled OR: 0.95, 95% CI: 0.93–0.98) or gestational diabetes (pooled OR: 1.06, 95% CI: 0.93–1.20).ConclusionShort IPI (IPI < 6 months) can lead to adverse perinatal outcomes, while it is not a risk factor for gestational diabetes and gestational hypertension. Therefore, more high-quality studies covering more comprehensive indicators of maternal and perinatal pregnancy outcomes are needed to ameliorate the pregnancy policy for women of childbearing age.</p

Table_2_Short interpregnancy interval can lead to adverse pregnancy outcomes: A meta-analysis.pdf

BackgroundThe evidence of some previous papers was insufficient in studying the causal association between interpregnancy interval (IPI) and adverse pregnancy outcomes. In addition, more literature have been updated worldwide during the last 10 years.MethodsEnglish and Chinese articles published from January 1980 to August 2021 in the databases of PubMed, Cochrane Library, Ovid, Embase, China Biology Medicine disc (CBM), and China National Knowledge Infrastructure (CNKI) were searched. Then following the inclusion and exclusion criteria, we screened the articles. Utilizing the Newcastle–Ottawa Scale (NOS), we evaluated the quality of the included articles. The literature information extraction table was set up in Excel, and the meta-analysis was performed with Stata 16.0 software (Texas, USA).ResultsA total of 41 articles were included in the meta-analysis, and NOS scores were four to eight. The short IPI after delivery was the risk factor of preterm birth (pooled odds ratio 1.49, 95% confidence interval 1.42–1.57), very preterm birth (pooled OR: 1.82, 95% CI: 1.55–2.14), low birth weight (pooled OR: 1.33, 95% CI: 1.24–1.43), and small for gestational age (pooled OR: 1.14, 95% CI: 1.07–1.21), offspring death (pooled OR: 1.60, 95% CI: 1.51–1.69), NICU (pooled OR: 1.26, 95% CI: 1.01–1.57), and congenital abnormality (pooled OR: 1.10, 95% CI: 1.05–1.16), while was not the risk factor of gestational hypertension (pooled OR: 0.95, 95% CI: 0.93–0.98) or gestational diabetes (pooled OR: 1.06, 95% CI: 0.93–1.20).ConclusionShort IPI (IPI < 6 months) can lead to adverse perinatal outcomes, while it is not a risk factor for gestational diabetes and gestational hypertension. Therefore, more high-quality studies covering more comprehensive indicators of maternal and perinatal pregnancy outcomes are needed to ameliorate the pregnancy policy for women of childbearing age.</p