7,911 research outputs found
Electronic structure and superconductivity of BiS2-based compounds LaO1-xFxBiS2
Using the density-functional perturbation theory with structural
optimization, we investigate the electronic structure, phonon spectra, and
superconductivity of BiS2-based layered compounds LaO1-xFxBiS2. For
LaO0.5F0.5BiS2, the calculated electron-phonon coupling constant is equal to
lambda = 0.8, and obtained Tc = 9.1 K is very close to its experimental value,
indicating that it is a conventional electron-phonon superconductor
Studies on Agrobacterium-mediated genetic transformation of embryogenic suspension cultures of sweet potato
In this study, genetic transformation of embryogenic suspension cultures of sweet potato (Ipomoea batatas) cultivar Xu55-2 was conducted utilizing the Agrobacterium tumefaciens strain EHA105 thatcontains the binary vector pBIN19/SBD2 with SBD2 (starch binding domain 2) gene and neomycin phosphotransferase (NPT II) gene. The presence of the SBD2 gene in the genomic DNA of transgenicplants was verified by PCR amplification and confirmed by Southern blot analysis. Results suggested that cefotaxime (Cefo), at the concentration of 200 mg/L, was able to effectively suppress the growth ofAgrobacterium after co-cultivation. The optimal concentration for kanamycin (Kan) was 10 mg/L for selecting resistance calli, somatic embryo formation and plant regeneration. The highest frequency ofshoot induction (30.9%) was obtained on the MS medium containing 10 mg/L Kan, 200 mg/L Cefo, 1.0 mg/L abscisic acid (ABA) and 1.0 mg/L gibberellic acid (GA3)
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