Production of doubly heavy baryons via Higgs boson decays

We systematically analyzed the production of semi-inclusive doubly heavy baryons ($\Xi_{cc}$, $\Xi_{bc}$ and $\Xi_{bb}$) for the process $H^0 \rightarrow \Xi_{QQ'}+ \bar {Q'} + \bar {Q}$ through four main Higgs decay channels within the framework of non-relativistic QCD. The contributions from the intermediate diquark states, $\langle cc\rangle[^{1}S_{0}]_{\mathbf{6}}$, $\langle cc\rangle[^{3}S_{1}]_{\mathbf{\bar 3}}$, $\langle bc\rangle[^{3}S_{1}]_{\mathbf{\bar 3}/ \mathbf{6}}$, $\langle bc\rangle[^{1}S_{0}]_{\mathbf{\bar 3}/ \mathbf{6}}$, $\langle bb\rangle[^{1}S_{0}]_{\mathbf{6}}$ and $\langle bb\rangle[^{3}S_{1}]_{\mathbf{\bar 3}}$, have been taken into consideration. The differential distributions and three main sources of the theoretical uncertainties have been discussed. At the High Luminosity Large Hadron Collider, there will be about 0.43$\times10^4$ events of $\Xi_{cc}$, 6.32$\times10^4$ events of $\Xi_{bc}$ and 0.28$\times10^4$ events of $\Xi_{bb}$ produced per year. There are fewer events produced at the Circular Electron Positron Collider and the International Linear Collider, about $0.26\times 10^{2}$ events of $\Xi_{cc}$, $3.83\times 10^{2}$ events of $\Xi_{bc}$ and $0.17\times 10^{2}$ events of $\Xi_{bb}$ in operation.Comment: 15 pages, 3 figures, 7 table

Enhanced yeast one-hybrid screens to identify transcription factor binding to human DNA sequences

Identifying the sets of transcription factors (TFs) that regulate each human gene is a daunting task that requires integrating numerous experimental and computational approaches. One such method is the yeast one-hybrid (Y1H) assay, in which interactions between TFs and DNA regions are tested in the milieu of the yeast nucleus using reporter genes. Y1H assays involve two components: a 'DNA-bait' (e.g., promoters, enhancers, silencers, etc.) and a 'TF-prey,' which can be screened for reporter gene activation. Most published protocols for performing Y1H screens are based on transforming TF-prey libraries or arrays into DNA-bait yeast strains. Here, we describe a pipeline, called enhanced Y1H (eY1H) assays, where TF-DNA interactions are interrogated by mating DNA-bait strains with an arrayed collection of TF-prey strains using a high density array (HDA) robotic platform that allows screening in a 1,536 colony format. This allows for a dramatic increase in throughput (60 DNA-bait sequences against >1,000 TFs takes two weeks per researcher) and reproducibility. We illustrate the different types of expected results by testing human promoter sequences against an array of 1,086 human TFs, as well as examples of issues that can arise during screens and how to troubleshoot them.Accepted manuscrip