mRNA expression analysis by quantitative real-time RT-PCR.

<p>mRNA expression levels of NGF/β-actin, TrkA/β-actin, and SYN/β-actin in the VaD group were significantly lower than those in the control group. Expression levels in untransfected and NGF-TERT co-transfected BMSC transplanted VaD groups were significantly higher than those of the VaD group. Additionally, expression levels in rats treated with NGF-TERT co-transfected BMSCs were significantly higher than those in rats treated with untransfected BMSCs (*, <i>P<</i>0.05, n = 12).</p

Cell proliferation analysis was performed after cell culture for 1 month (<b>Figure 2a</b>) and 2 months (<b>Figure 2b</b>).

<p>The rate of proliferation in TERT-BMSCs was significantly increased compared to untransfected BMSCs and empty vector-transfected BMSCs following one month of cell culture (<i>P<</i>0.05) (Figure 2a). After long-term culture of 2 months, the rate of proliferation decreased in untransfected and empty-vector-transfected BMSCs but remained high in TERT-BMSCs (<i>P<</i>0.05) (Figure 2b).</p

Ultrastructure in the hippocampal CA1 area by transmission electron microscopy (×40,000).

<p>The synaptic structural parameters of the hippocampal CA1 area in each group are shown. PSD, postsynaptic density; SC, synaptic cleft; SAZ, synaptic active zone. (*<i>P<</i>0.05, n = 12).</p

Protein expression analysis by western blotting.

<p>After normalisation to actin, protein levels of NGF, TrkA, and SYN in the VaD group were significantly lower than those in the control group. The untransfected and NGF-TERT co-transfected BMSC transplanted VaD groups had significantly higher levels of these proteins than the VaD group. Additionally, rats treated with NGF-TERT co-transfected BMSCs had significantly higher levels of NGF, TrkA, and SYN than rats treated with untransfected BMSCs (*<i>P<</i>0.05, n = 12).</p

Primers in real-time RT-PCR analysis.

<p>Primers in real-time RT-PCR analysis.</p

Immunohistochemical analysis of protein expression.

<p>NGF-positive and TrkA-positive cells were detected in the hippocampus and cortex, and these proteins were most highly abundant in the cytoplasm of hippocampal cells. SYN protein expression was stronger in the emitting layer of the hippocampus CA1 area than in the molecular layer, and the outline of the cone cells showed minimal SYN. The average grey values of NGF, TrkA, and SYN proteins in the VaD group were significantly lower than those in the control group. The average grey values of untransfected and NGF-TERT co-transfected BMSCs transplanted VaD groups were higher than those of the VaD group. Additionally, grey values of NGF-TERT co-transfected BMSCs transplanted VaD rats were significantly higher than those of the untransfected BMSCs transplanted VaD rats (*<i>P<</i>0.05, n = 12).</p

Tribenzodecacyclene and Hexabenzodecacyclene

High-temperature, TiCl₄-catalyzed, triple aldol condensations of aceanthrenone <b>5</b> and acenaphthacenone <b>6</b> gave tribenzodecacyclene <b>3</b> and hexabenzodecacyclene <b>4</b>, respectively, in yields of 16 and 0.8%, respectively. Compound <b>3</b> is a red, crystalline solid that is stable under ordinary conditions; its X-ray structure reveals it to be a strongly pitched, <i>C</i>₃-symmetric, molecular propeller. In contrast, the more highly strained compound <b>4</b> is a blue-black solid whose solutions are unstable to air and light. Its simple NMR spectra, as well as HDFT calculations, indicate that it is a <i>D</i>₃-symmetric molecular propeller

Synthesis and Chiroptical Properties of Arylimines of Cholest-4-ene-3,6-dione

In the hope of generating a new class of materials with large optical rotations, various arylamines were condensed with cholest-4-ene-3,6-dione (<b>2</b>) to give 3-arylimino steroids. These compounds possess moderately high specific rotations ([α]_D ∼300–800) and strong circular dichroism. One such derivative, 3-(4-methoxyphenylimino)­cholest-4-en-6-one (<b>3</b>), crystallizes as the (<i>E</i>)-imine and upon dissolution undergoes mutarotation to an equilibrium mixture of (<i>E</i>)- and (<i>Z</i>)-isomers with a half-life of approximately 1 h at room temperature, as judged by both NMR spectroscopy and polarimetry

Synthesis and Chiroptical Properties of Arylimines of Cholest-4-ene-3,6-dione

In the hope of generating a new class of materials with large optical rotations, various arylamines were condensed with cholest-4-ene-3,6-dione (<b>2</b>) to give 3-arylimino steroids. These compounds possess moderately high specific rotations ([α]_D ∼300–800) and strong circular dichroism. One such derivative, 3-(4-methoxyphenylimino)­cholest-4-en-6-one (<b>3</b>), crystallizes as the (<i>E</i>)-imine and upon dissolution undergoes mutarotation to an equilibrium mixture of (<i>E</i>)- and (<i>Z</i>)-isomers with a half-life of approximately 1 h at room temperature, as judged by both NMR spectroscopy and polarimetry

Synthesis and Chiroptical Properties of Arylimines of Cholest-4-ene-3,6-dione

In the hope of generating a new class of materials with large optical rotations, various arylamines were condensed with cholest-4-ene-3,6-dione (<b>2</b>) to give 3-arylimino steroids. These compounds possess moderately high specific rotations ([α]_D ∼300–800) and strong circular dichroism. One such derivative, 3-(4-methoxyphenylimino)­cholest-4-en-6-one (<b>3</b>), crystallizes as the (<i>E</i>)-imine and upon dissolution undergoes mutarotation to an equilibrium mixture of (<i>E</i>)- and (<i>Z</i>)-isomers with a half-life of approximately 1 h at room temperature, as judged by both NMR spectroscopy and polarimetry