Dcr-1 Maintains Drosophila Ovarian Stem Cells

SummaryMicroRNAs (miRNAs) regulate gene expression by controlling the turnover, translation, or both of specific mRNAs. In Drosophila, Dicer-1 (Dcr-1) is essential for generating mature miRNAs from their corresponding precursors. Because miRNAs are known to modulate developmental events, such as cell fate determination and maintenance in many species, we investigated whether a lack of Dcr-1 would affect the maintenance of stem cells (germline stem cells, GSCs; somatic stem cells, SSCs) in the Drosophila ovary by specifically removing its function from the stem cells. Our results show that dcr-1 mutant GSCs cannot be maintained and are lost rapidly from the niche without discernable features of cell death, indicating that Dcr-1 controls GSC self-renewal but not survival. bag of marbles (bam), the gene that encodes an important differentiating factor in the Drosophila germline, however, is not upregulated in dcr-1 mutant GSCs, and its removal does not slow down dcr-1 mutant GSC loss, suggesting that Dcr-1 controls GSC self-renewal by repressing a Bam-independent differentiation pathway. Furthermore, Dcr-1 is also essential for the maintenance of SSCs in the Drosophila ovary. Our data suggest that miRNAs produced by Dcr-1 are required for maintaining two types of stem cells in the Drosophila ovary

BSG alignment of SDSS galaxy groups

We study the alignment signal between the distribution of brightest satellite galaxies (BSGs) and the major axis of their host groups using SDSS group catalog constructed by Yang et al. (2007). After correcting for the effect of group ellipticity, a statistically significant (~ 5\sigma) major-axis alignment is detected and the alignment angle is found to be 43.0 \pm 0.4 degrees. More massive and richer groups show stronger BSG alignment. The BSG alignment around blue BCGs is slightly stronger than that around red BCGs. And red BSGs have much stronger major-axis alignment than blue BSGs. Unlike BSGs, other satellites do not show very significant alignment with group major axis. We further explore the BSG alignment in semi-analytic model (SAM) constructed by Guo et al. (2011). We found general good agreement with observations: BSGs in SAM show strong major-axis alignment which depends on group mass and richness in the same way as observations; and none of other satellites exhibit prominent alignment. However, discrepancy also exists in that the SAM shows opposite BSG color dependence, which is most probably induced by the missing of large scale environment ingredient in SAM. The combination of two popular scenarios can explain the detected BSG alignment. The first one: satellites merged into the group preferentially along the surrounding filaments, which is strongly aligned with the major axis of the group. The second one: BSGs enter their host group more recently than other satellites, then will preserve more information about the assembling history and so the major-axis alignment. In SAM, we found positive evidence for the second scenario by the fact that BSGs merged into groups statistically more recently than other satellites. On the other hand, although is opposite in SAM, the BSG color dependence in observation might indicate the first scenario as well.Comment: 8 pages, 11 figures, ApJ accepte

Polyacrylonitrile nanofibre yarn; electrospinning and their post-drawing behaviour

Polyacrylontrile nanofibre yarns have been successfully produced from an electrospinning setup composing positively and negatively charged spinnerets, a rotating funnel and a yarn winder. Through hot drawing, yarns show compact morphology and improved uniformity and have a significant decrease in both yarn and fibre diameters. The hot drawing has improved the molecular orientation and crystallinity of the fibres. The yarn drawn to 5 times of its original length has been found to have the highest tensile strength and modulus

Numerical investigation on rules of fracture propagation during hydraulic fracturing in heterogeneous coal-rock mass

To investigate rules of fracture propagation during hydraulic fracturing in heterogeneous coal-rock mass, a new mathematical model for hydraulic fracturing with seepage-damage coupling and its numerical algorithm are proposed. The rules of coal-rock mass heterogeneity, confining pressure, beforehand hydraulic slotting, and non-symmetric pressure gradient on fracture propagation are investigated. Numerical results show the following: (1) Compared to homogeneous coal-rock mass, the fracture propagation pattern exhibits a more zig-zag characteristic and the fracture initiation pressure is reduced in heterogeneous coal-rock mass. (2) Fracture propagation during borehole fracturing is mainly controlled by confining pressure ratio, and the fracture would propagate along the path with least resistance in coal-rock mass. (3) During hydraulic fracturing with beforehand hydraulic slotting, fracture propagation pattern would become more complex with slotting length increasing; the propagation direction of fracture is primarily controlled by principal stress difference, the larger of principal stress difference, the more difficult of oriented fracturing. (4) Non-symmetric pressure gradient can reduce breakdown pressure and influence fracture propagation pattern, which provides a beneficial guide for oriented fracturing. The simulation results are consistent with the theoretical solutions and experimental observations, which is promising to guide field operation of hydraulic fracturing to improve coalbed methane extraction

A novel PCR strategy for high-efficiency, automated site-directed mutagenesis

We have developed a novel three-primer, one-step PCR-based method for site-directed mutagenesis. This method takes advantage of the fact that template plasmid DNA cannot be efficiently denatured at its reannealing temperature (T(ra)), which is otherwise a troublesome problem in regular PCR. Two flanking primers and one mutagenic primer with different melting temperatures (T(m)) are used together in a single PCR tube continuously without any intervention. A single-stranded mutagenic DNA (smDNA) is synthesized utilizing the high T(m) mutagenic primer at a high annealing temperature, which prevents the priming of the low T(m) primers (i.e. the two flanking primers). A megaprimer is then produced using this smDNA as the template at a denaturing temperature that prevents wild-type template DNA activity. The desired mutant DNA is then obtained by cycling again through these first two steps, resulting in a mutagenic efficiency of 100% in all tested cases. This highly automated method not only eliminates the necessity of any intermediate manipulation and accomplishes the mutagenesis process in a single round of PCR but, most notably, enables complete success of mutagenesis. This novel method is also both cost and time efficient and fully automated

Communication: Rigorous Quantum Dynamics of O + O₂ Exchange Reactions on an Ab Initio Potential Energy Surface Substantiate the Negative Temperature Dependence of Rate Coefficients

The kinetics and dynamics of several O + O2 isotope exchange reactions have been investigated on a recently determined accurate global O3 potential energy surface using a time-dependent wave packet method. The agreement between calculated and measured rate coefficients is significantly improved over previous work. More importantly, the experimentally observed negative temperature dependence of the rate coefficients is for the first time rigorously reproduced theoretically. This negative temperature dependence can be attributed to the absence in the new potential energy surface of a submerged reef structure, which was present in all previous potential energy surfaces. In addition, contributions of rotational excited states of the diatomic reactant further accentuate the negative temperature dependence

Desertification Reversal Promotes the Complexity of Plant Community by Increasing Plant Species Diversity of Each Plant Functional Type

Desertification reversal is globally significant for the sustainable development of land resources. However, the mechanisms of desertification reversal at the level of plant community are still unclear. We hypothesized that desertification reversal has clear effects on plant community composition, plant functional types (PFTs), and other vegetation characteristics, including plant diversity and biomass, and their changes in the early stages of reversal are more dramatic than in later stages. We investigated the vegetation of four to five different stages of desertification reversal at each of seven large study sites in southwestern Mu Us Sandy Land, China. The results show that the dominant species in very severe desertification areas were replaced by perennial grasses in potential desertification areas. The importance values of annual forbs and perennial sub-shrubs decreased dramatically (from 42.59 and 32.98 to 22.13 and 5.54, respectively), whereas those of perennial grasses and perennial forbs increased prominently (from 13.26 and 2.71 to 53.94 and 11.79, respectively) with the reversal of desertification. Desertification reversal increased the complexity of plant community composition by increasing plant species in each PFT, and C3 plants replaced C4 plants to become the dominant PFT with reversal. Plant species richness and species diversity rose overall, and aboveground plant biomass significantly (p < 0.05) increased with the reversal of desertification. Most vegetation characteristics changed more strikingly in the early stages of desertification reversal than in later stages. Our results indicate that the type and composition of the plant community were dramatically affected by desertification reversal. Anthropogenic measures are more applicable to being employed in early stages than in later stages, and Amaranthaceae C4 plants are suggested to be planted in mobile dunes for the acceleration of desertification reversal. This study is useful for designing strategies of land management and ecological restoration in arid and semiarid regions

A revisit of superconductivity in 4HbH_b-TaS2−2x_{2-2x}Se2x_{2x} single crystals

Previous investigations of 4$H_b$-TaS$_{2-2x}$Se$_{2x}$ mainly focused on the direct competition between superconductivity and charge density wave (CDW). However, the superconductivity itself, although has been prominently enhanced by isovalent Se substitution, has not been adequately investigated. Here, we performed a detailed electrical transport measurement down to 0.1 K on a series of 4$H_b$-TaS$_{2-2x}$Se$_{2x}$ single crystals. A systematic fitting of the temperature-dependent resistance demonstrates that the decreased Debye temperatures ($\Theta_{D}$) and higher electron-phonon coupling constants ($\lambda_{e-p}$) at the optimal Se doping content raise the superconducting transition temperature ($T_c$). Additionally, we discovered that the incorporation of Se diminishes the degree of anisotropy of the superconductivity in the highly layered structure. More prominently, a comprehensive analysis of the vortex liquid phase region reveals that the optimally doped sample deviates from the canonical 2D Tinkham prediction but favors a linear trend with the variation of the external magnetic field. These findings emphasize the importance of interlayer interaction in this segregated superconducting-Mott-insulating system.Comment: 11 pages, 5 figure

Downwelling wind, tides, and estuarine plume dynamics

Author Posting. © American Geophysical Union, 2016. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 121 (2016): 4245–4263, doi:10.1002/2015JC011475.The estuarine plume dynamics under a downwelling-favorable wind condition were examined in the windy dry season of the Pearl River Estuary (PRE) using the PRE primitive-equation Finite-Volume Community Ocean Model (FVCOM). The wind and tide-driven estuarine circulation had a significant influence on the plume dynamics on both local and remote scales. Specifically, the local effect of downwelling-favorable winds on the plume was similar to the theoretical descriptions of coastal plumes, narrowing the plume width, and setting up a vertically uniform downstream current at the plume edge. Tides tended to reduce these plume responses through local turbulent mixing and advection from upstream regions, resulting in an adjustment of the isohalines in the plume and a weakening of the vertically uniform downstream current. The remote effect of downwelling-favorable winds on the plume was due to the wind-induced estuarine sea surface height (SSH), which strengthened the estuarine circulation and enhanced the plume transport accordingly. Associated with these processes, tide-induced mixing tended to weaken the SSH gradient and thus the estuarine circulation over a remote influence scale. Overall, the typical features of downwelling-favorable wind-driven estuarine plumes revealed in this study enhanced our understanding of the estuarine plume dynamics under downwelling-favorable wind conditions.National Natural Science Foundation of China Grant Number: (41206005); Ocean Public Welfare Scientific Research Project, State Oceanic Administration of the People's Republic of China Grant Number: (201305019-3)2016-12-2