379 research outputs found
Research on real-time detection algorithm of safety helmets in complex operating environment
In order to solve the problems of low detection accuracy when the background of safety helmets is complex at construction sites,
and the safety helmet target is too small to be easily detected, this paper proposes a real-time detection algorithm for safety helmets in complex working environments based on the YOLOv5 framework. An improved YOLOv5 detection algorithm is proposed to address the issues
of missing safety helmets and low detection accuracy in the construction environment. Adding an attention mechanism to the YOLOv5 backbone network, adding a detection layer at the neck of the network, and integrating an ASFF module at the neck of the network have better
detection performance when facing complex backgrounds and dense helmet detection; The experimental results show that compared to the
original YOLOv5 model, the improved average accuracy has increased by 2.4%, reaching 91.3%, effectively improving the detection ability
of safety helmets in complex environments
CDC5, a DNA binding protein, positively regulates posttranscriptional processing and/or transcription of primary microRNA transcripts
CDC5 is a MYB-related protein that exists in plants, animals, and fungi. In Arabidopsis, CDC5 regulates both growth and immunity through unknown mechanisms. Here, we show that CDC5 from Arabidopsis positively regulates the accumulation of microRNAs (miRNAs), which control many biological processes including development and adaptations to environments in plants. CDC5 interacts with both the promoters of genes encoding miRNAs (MIR) and the DNA-dependent RNA polymerase II. As a consequence, lack of CDC5 reduces the occupancy of polymerase II at MIR promoters, as well as MIR promoter activities. In addition, CDC5 is associated with the DICER–LIKE1 complex, which generates miRNAs from their primary transcripts and is required for efficient miRNA production. These results suggest that CDC5 may have dual roles in miRNA biogenesis: functioning as a positive transcription factor of MIR and/or acting as a component of the DICER–LIKE1 complex to enhance primary miRNA processing
URLLC packet management for packetized predictive control
Packetized predictive control (PPC) is an effective
solution to ensure the robustness of the control
system over unreliable wireless links. However, conventional
wireless transmission methods in PPC suffer from either
high wireless resource consumption or poor performance of
real-time control due to the separately design of the two
parts. To deal with the issue, we propose a communicationcontrol
co-design approach to achieve good trade-off between
real-time control performance and communication
energy efficiency. Our results demonstrate the advantages
of the communication-control co-design
Methylation protects microRNAs from an AGO1- associated activity that uridylates 5′ RNA fragments generated by AGO1 cleavage
In plants, methylation catalyzed by HEN1 (small RNA methyl transferase) prevents microRNAs (miRNAs) from degradation triggered by uridylation. Howmethylation antagonizes uridylation of miRNAs in vivo is not well understood. In addition, 5′ RNA fragments (5′ fragments) produced by miRNA-mediated RNA cleavage can be uridylated in plants and animals. However, the biological significance of this modification is unknown, and enzymes uridylating 5′ fragments remain to be identified. Here, we report that in Arabidopsis, HEN1 suppressor 1 (HESO1, a miRNA nucleotidyl transferase) uridylates 5′ fragments to trigger their degradation.We also show that Argonaute 1 (AGO1), the effector protein of miRNAs, interacts with HESO1 through its Piwi/Argonaute/Zwille and PIWI domains, which bind the 3′ end of miRNA and cleave the target mRNAs, respectively. Furthermore, HESO1 is able to uridylate AGO1-bound miRNAs in vitro. miRNA uridylation in vivo requires a functional AGO1 in hen1, in which miRNA methylation is impaired, demonstrating that HESO1 can recognize its substrates in the AGO1 complex. On the basis of these results, we propose that methylation is required to protect miRNAs from AGO1-associated HESO1 activity that normally uridylates 5′ fragments
Demonstration of a 280-Gbit/s free-space SDM communications link utilizing plane-wave spatial multiplexing
We demonstrate a 280-Gbit/s free-space SDM
communications link incorporating a set of independent
tilted truncated plane-waves, each generated by a single
mode fiber placed at the back-focal plane of a spherical
lens. Each of the 7 tilted plane-wave channels are
encoded with a 40-Gbit/s 16-QAM signal. Our approach
comprises two identical linear fiber-arrays placed
approximately 5 m apart. As each fiber array is placed at
the back-focal-plane of a spherical lens, each fiber array
is effectively placed in a conjugate image plane of the
other. A channel crosstalk less than 26 dB is shown, with
a bit-error-rate below the FEC threshold of 3.8 × 10−3
Tissue specific induction of p62/sqstm1 by farnesoid X receptor
Background: Farnesoid X Receptor (FXR) is a member of the nuclear receptor superfamily and is a ligand-activated transcription factor essential for maintaining liver and intestinal homeostasis. FXR is protective against carcinogenesis and inflammation in liver and intestine as demonstrated by the development of inflammation and tumors in the liver and intestine of FXR knock-out mice. However, mechanisms for the protective effects of FXR are not completely understood. This study reports a novel role of FXR in regulating expression of Sqstm1, which encodes for p62 protein. p62 plays an important role in maintaining cellular homeostasis through selective autophagy and activating signal transduction pathways, such as NF-κB to support cell survival and caspase-8 to initiate apoptosis. FXR regulation of Sqstm1 may serve as a protective mechanism. Methods and Results: This study showed that FXR bound to the Sqstm1 gene in both mouse livers and ileums as determined by chromatin immunoprecipitation. In addition, FXR activation enhanced transcriptional activation of Sqstm1 in vitro. However, wild-type mice treated with GW4064, a synthetic FXR ligand, showed that FXR activation induced mRNA and protein expression of Sqstm1/p62 in ileum, but not in liver. Interestingly, FXR-transgenic mice showed induced mRNA expression of Sqstm1 in both liver and ileum compared to wild-type mice. Conclusions: Our current study has identified a novel role of FXR in regulating the expression of p62, a key factor in protein degradation and cell signaling. Regulation of p62 by FXR indicates tissue-specific and gene-dosage effects. Furthermore, FXR-mediated induction of p62 may implicate a protective mechanism of FXR. © 2012 Williams et al
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