8 research outputs found
Preparation and Characterization of Chitosan/β-Glycerophosphate Thermal-Sensitive Hydrogel Reinforced by Graphene Oxide
Thermal-sensitive hydrogel based on chitosan (CS) and β-glycerophosphate (GP) has shown good biocompatibility and biodegradability. But the application of such hydrogel is limited due to its poor mechanical property. Recently, graphene oxide(GO) is widely used as a reinforcement agent to prepare nanocomposites with different polymers for improving the properties of the materials. In this study, CS/GP-based hydrogels with different weight ratio of GO/CS (0.5, 1, 2%) were fabricated. The gelation time of the hydrogels at body temperature was evaluated by tube inverting method. The gelation process during heating was monitored by rheological measurement. The morphology, porosities, chemical structure, swelling properties of the lyophilized hydrogels were investigated by scanning electron microscopy, liquid displacement method, Fourier transform infrared spectroscopy and gravimetric method. Mechanical property of the hydrogels was analyzed by rheological measurement and unconfined compression test. MC3T3-E1 mouse pre-osteoblast cell line was used to assess the biological properties of the hydrogels. The results obtained from those assessments revealed that the addition of GO into CS/GP improved the properties of the prepared hydrogels without changing the high porous and interconnected microstructure and swelling ability of the hydrogels. The gelation time at body temperature was significantly reduced by nearly 20% with the addition of small amount of GO (0.5% weight ratio of CS). The mechanical properties of the hydrogels containing GO were improved significantly over that of CS/GP. The storage (G′)/loss (G″) moduli of the hydrogels with GO were 1.12 to 1.69 times that of CS/GP at the gelling temperature. The Young's modulus of 0.5%GO/CS/GP hydrogel is 1.76 times that of CS/GP. Moreover, the 0.5%GO/CS/GP hydrogel revealed remarkable biological affinity such as cellular attachment, viability and proliferation. All of these results suggest that 0.5%GO/CS/GP hydrogel has great potential for practical application in biomedical field
Influence of Concentration of Sodium Metasilicate and Descaling on the High Temperature Lubricating Effects Evaluated by Hot Rolling Mill
Lubricant is vital to improve energy efficiency and workpiece durability for the moving counterpart. High-temperature lubricants are important for the hot rolling process to reduce the rolling force and protect the roller and the strips. The current paper concerns eco-friendly sodium metasilicate as a high-temperature lubricant. A hot rolling mill is employed to evaluate the lubrication effect of sodium metasilicate. The influence of crucial factors of concentration of lubricant and descaling is discussed; the rolled surface was analyzed by scanning electron microscopy, energy dispersive spectroscopy, and 3D profilometer. The results depict that the sodium metasilicate can reduce the rolling force by about 7.8% when the concentration of sodium metasilicate is 18% and above, and descaling of the hot stripe makes the lubrication effect more effective, which can reach a 12.7% reduction in the rolling force. This lubrication is attributed to the formed melts of the sodium silicate layer that offers an easy shearing interface. For the un-descaled samples, the lubricant will be compacted and mixed with the oxide scale, and weakens the lubrication effect. This work suggests that sodium metasilicate can be a high-temperature lubricant for hot rolling; descaling is vital, not only for the quality of the product but also for the efficiency of the lubricant. This work will also be useful for the concentration selection of glass lubricant
Enhanced Osseointegration of Porous Titanium Modified with Zeolitic Imidazolate Framework‑8
Nanoscale zeolitic imidazolate framework-8
(ZIF-8)-modified titanium
(ZIF-8@AHT) can enhance osteogenesis in vitro. In this study, we systematically
and quantitatively examined the effects of ZIF-8@AHT on osteogenesis,
and investigated its ability to form bone in vivo. First, we coated
various quantities of nanoscale ZIF-8 crystals on alkali- and heat-treated
titanium (AHT) by controlling the concentration of the synthesis solution.
We then characterized the ZIF-8@AHT materials using scanning electron
microscopy (SEM), powder X-ray diffraction (XRD), Fourier transform
infrared spectroscopy (FT-IR), and determination of the sessile drop
contact angle. To illustrate the combined effects of micro/nanotopography
and ZIF-8@AHT composition on bone regeneration, we cultured MC3T3-E1
preosteoblast cells on various titanium substrates in vitro by setting
pure titanium (Ti) and AHT as control groups. The ZIF-8@AHTs enhanced
cell bioactivity compared with AHT and Ti, as evidenced by increased
extracellular matrix (ECM) mineralization, collagen secretion and
the upregulated expression of osteogenic genes (<i>Alp</i>, <i>Col1</i>, <i>Opg</i>, and <i>Runx2</i>) and osteogenesis-related proteins (ALP and OPG). ZIF-8@AHT-1/8
exhibited better osteogenic activity compared with the other ZIF-8@AHT
groups investigated. We subsequently inserted Ti, AHT, and ZIF-8@AHT-1/8
implants into the healed first molars (M1s) of mice, and found that
ZIF-8@AHT-1/8 also promoted osseointegration at the bone–implant
interface. These results suggest that ZIF-8@AHT-1/8 has great potential
for practical application in implant modification
Enhanced Osseointegration of Porous Titanium Modified with Zeolitic Imidazolate Framework‑8
Nanoscale zeolitic imidazolate framework-8
(ZIF-8)-modified titanium
(ZIF-8@AHT) can enhance osteogenesis in vitro. In this study, we systematically
and quantitatively examined the effects of ZIF-8@AHT on osteogenesis,
and investigated its ability to form bone in vivo. First, we coated
various quantities of nanoscale ZIF-8 crystals on alkali- and heat-treated
titanium (AHT) by controlling the concentration of the synthesis solution.
We then characterized the ZIF-8@AHT materials using scanning electron
microscopy (SEM), powder X-ray diffraction (XRD), Fourier transform
infrared spectroscopy (FT-IR), and determination of the sessile drop
contact angle. To illustrate the combined effects of micro/nanotopography
and ZIF-8@AHT composition on bone regeneration, we cultured MC3T3-E1
preosteoblast cells on various titanium substrates in vitro by setting
pure titanium (Ti) and AHT as control groups. The ZIF-8@AHTs enhanced
cell bioactivity compared with AHT and Ti, as evidenced by increased
extracellular matrix (ECM) mineralization, collagen secretion and
the upregulated expression of osteogenic genes (<i>Alp</i>, <i>Col1</i>, <i>Opg</i>, and <i>Runx2</i>) and osteogenesis-related proteins (ALP and OPG). ZIF-8@AHT-1/8
exhibited better osteogenic activity compared with the other ZIF-8@AHT
groups investigated. We subsequently inserted Ti, AHT, and ZIF-8@AHT-1/8
implants into the healed first molars (M1s) of mice, and found that
ZIF-8@AHT-1/8 also promoted osseointegration at the bone–implant
interface. These results suggest that ZIF-8@AHT-1/8 has great potential
for practical application in implant modification