Effect of culture residence time on substrate uptake and storage by a pure culture of Thiothrix (CT3 strain) under continuous or batch feeding

A pure culture of the filamentous bacterium Thiothrix, strain CT3, was aerobically cultured in a chemostat under continuous acetate feeding at three different culture residence times (RT 6, 12 or 22 d) and the same volumetric organic load rate (OLR 0.12 gCOD/L/d). Cells cultured at decreasing RT in the chemostat had an increasing transient response to acetate spikes in batch tests. The maximum specific acetate removal rate increased from 25 to 185 mgCOD/gCOD/h, corresponding to a 1.8 to 8.1 fold higher respective steady-state rate in the chemostat. The transient response was mainly due to acetate storage in the form of poly(3-hydroxybutyrate) (PHB), whereas no growth response was observed at any RT. Interestingly, even though the storage rate also decreased as the RT increased, the storage yield increased from 0.41 to 0.50 COD/COD. This finding does not support the traditional view that storage plays a more important role as the transient response increases. The transient response of the steady-state cells was much lower than in cells cultured under periodic feeding (at 6 d RT, from 82 to 247 mgCOD/gCOD/h), with the latter cells showing both storage and growth responses. On the other hand, even though steady-state cells had no growth response and their storage rate was also less, steady-state cells showed a higher storage yield than cells cultured under dynamic feeding. This suggests that in Thiothrix strain CT3, the growth response is triggered by periodic feeding, whereas the storage response is a constitutive mechanism, independent from previous acclimation to transient conditions

Table_1_Linking life table and predation rate for evaluating temperature effects on Orius strigicollis for the biological control of Frankliniella occidentalis.DOCX

IntroductionOrius spp. are generalist predators released in horticultural and agricultural systems to control thrips. Understanding the effects of temperature on the development, predation rate, and population dynamics of Orius is essential for identifying the optimal timing of Orius release for establishing an adequate population to facilitate synchrony with thrips population growth and to prevent thrips outbreaks. The biological control efficiency of natural enemies as well as predator–prey relationships can be precisely described by integrating life table parameters and the predation rate.MethodsIn this study, the demographic features of Orius strigicollis fed on 2nd instar nymphs of western flower thrips (WFT), Frankliniella occidentalis, were compared at 18.5, 23.5, 27, and 33°C using the TWOSEX-MSChart program. The CONSUME-MSChart program was used to examine predation rates under different temperatures (18.5, 23.5, and 27°C).ResultsThe results showed no significant difference in fecundity among those reared at 18.5, 23.5, and 27°C, but fecundity at these temperatures was significantly higher than that at 33°C. The intrinsic rate of increase (r), finite rate of increase (λ), and net reproduction rate (R0) were the highest at 27°C. The net predation rate (C0) and transformation rate (Qp) were significantly higher at 18.5°C (C0 = 168.39 prey/predator, Qp = 8.22) and 23.5°C (C0 = 140.49 prey/predator, Qp = 6.03) than at 27°C (C0 = 138.39 prey/predator, Qp= 3.81); however, the finite predation rate (ω) showed the opposite trend. In addition to temperature, the stage of O. strigicollis at release can affect population dynamics.DiscussionOur study showed that temperature influenced the demographic traits and predation rates of O. strigicollis. When planning a release, the stage of O. strigicollis and temperature should be taken into account to establish an adequate population for the control of WFT.</p

Metagenomic Detection of Viruses in Aerosol Samples from Workers in Animal Slaughterhouses

<div><p>Published studies have shown that workers in animal slaughterhouses are at a higher risk of lung cancers as compared to the general population. No specific causal agents have been identified, and exposures to several chemicals have been examined and found to be unrelated. Evidence suggests a biological aetiology as the risk is highest for workers who are exposed to live animals or to biological material containing animal faeces, urine or blood. To investigate possible biological exposures in animal slaughterhouses, we used a metagenomic approach to characterise the profile of organisms present within an aerosol sample. An assessment of aerosol exposures for individual workers was achieved by the collection of personal samples that represent the inhalable fraction of dust/bioaerosol in workplace air in both cattle and sheep slaughterhouses. Two sets of nine personal aerosol samples were pooled for the cattle processing and sheep processing areas respectively, with a total of 332,677,346 sequence reads and 250,144,492 sequence reads of 85 bp in length produced for each. Eukaryotic genome sequence was found in both sampling locations, and bovine, ovine and human sequences were common. Sequences from WU polyomavirus and human papillomavirus 120 were detected in the metagenomic dataset from the cattle processing area, and these sequences were confirmed as being present in the original personal aerosol samples. This study presents the first metagenomic description of personal aerosol exposure and this methodology could be applied to a variety of environments. Also, the detection of two candidate viruses warrants further investigation in the setting of occupational exposures in animal slaughterhouses.</p></div

Identity of virus contigs as reported by BLASTN search against the non-redundant genbank nucleotide database, and subsequent taxonomic assignment by MEGAN.

<p>Identity of virus contigs as reported by BLASTN search against the non-redundant genbank nucleotide database, and subsequent taxonomic assignment by MEGAN.</p

Summary of mapping statistics for WU polyomavirus and human papillomavirus 120 sequences identified in the cattle processing area aerosol sample, when mapped to reference genomes GU296398.1 for WUPyV, and JQ963485.1 for HPV120, using the Bowtie 2 software [28].

<p>Summary of mapping statistics for WU polyomavirus and human papillomavirus 120 sequences identified in the cattle processing area aerosol sample, when mapped to reference genomes GU296398.1 for WUPyV, and JQ963485.1 for HPV120, using the Bowtie 2 software <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072226#pone.0072226-Langmead1" target="_blank">[28]</a>.</p