Media 2: SIFT flow for large-displacement object tracking

Originally published in Applied Optics on 20 September 2014 (ao-53-27-6194

Model-driven development of interactive and integrated 2D and 3D user interfaces using MML

While there is a lot of research done in the area of 2D or 3D user interfaces (UIs) construction, comparatively little is known about systematic approaches to designing and developing integrated 2D/3D UIs and applications. The previously developed multimedia modeling language (MML) provides a top down approach for a model driven development of 2D/3D UIs and applications. The MML structure model and media components provide support for including X3D based content and automatic generation of application skeletons. We use a work instruction manual for a woodchipper as an example to illustrate how to apply MML. We discuss the ramifications of this approach and opportunities for some improvements

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Data_Sheet_1_Lactobacillus plantarum surface-displayed FomA (Fusobacterium nucleatum) protein generally stimulates protective immune responses in mice.pdf

A significant correlation is observed between Fusobacterium nucleatum (F. nucleatum) and the evolution of inflammatory bowel disease (IBD). Particularly, FomA, a critical pathogenic element of F. nucleatum, inflicts substantial detriment to human intestinal health. Our research focused on the development of recombinant Lactobacillus plantarum that expresses FomA protein, demonstrating its potential in protecting mice from severe IBD induced by F. nucleatum. To commence, two recombinant strains, namely L. plantarum NC8-pSIP409-pgsA'-FomA and NC8-pSIP409-FnBPA-pgsA'-FomA, were successfully developed. Validation of the results was achieved through flow cytometry, ELISA, and MTT assays. It was observed that recombinant L. plantarum instigated mouse-specific humoral immunity and elicited mucosal and T cell-mediated immune responses. Significantly, it amplified the immune reaction of B cells and CD4+T cells, facilitated the secretion of cytokines such as IgA, IL4, and IL10, and induced lymphocyte proliferation in response to FomA protein stimulation. Finally, we discovered that administering recombinant L. plantarum could protect mice from severe IBD triggered by F. nucleatum, subsequently reducing pathological alterations and inflammatory responses. These empirical findings further the study of an innovative oral recombinant Lactobacillus vaccine.</p

Data_Sheet_2_Lactobacillus plantarum surface-displayed FomA (Fusobacterium nucleatum) protein generally stimulates protective immune responses in mice.pdf

A significant correlation is observed between Fusobacterium nucleatum (F. nucleatum) and the evolution of inflammatory bowel disease (IBD). Particularly, FomA, a critical pathogenic element of F. nucleatum, inflicts substantial detriment to human intestinal health. Our research focused on the development of recombinant Lactobacillus plantarum that expresses FomA protein, demonstrating its potential in protecting mice from severe IBD induced by F. nucleatum. To commence, two recombinant strains, namely L. plantarum NC8-pSIP409-pgsA'-FomA and NC8-pSIP409-FnBPA-pgsA'-FomA, were successfully developed. Validation of the results was achieved through flow cytometry, ELISA, and MTT assays. It was observed that recombinant L. plantarum instigated mouse-specific humoral immunity and elicited mucosal and T cell-mediated immune responses. Significantly, it amplified the immune reaction of B cells and CD4+T cells, facilitated the secretion of cytokines such as IgA, IL4, and IL10, and induced lymphocyte proliferation in response to FomA protein stimulation. Finally, we discovered that administering recombinant L. plantarum could protect mice from severe IBD triggered by F. nucleatum, subsequently reducing pathological alterations and inflammatory responses. These empirical findings further the study of an innovative oral recombinant Lactobacillus vaccine.</p

Induction, Purification and Characterization of a Novel Manganese Peroxidase from <i>Irpex lacteus</i> CD2 and Its Application in the Decolorization of Different Types of Dye

<div><p>Manganese peroxidase (MnP) is the one of the important ligninolytic enzymes produced by lignin-degrading fungi which has the great application value in the field of environmental biotechnology. Searching for new MnP with stronger tolerance to metal ions and organic solvents is important for the maximization of potential of MnP in the biodegradation of recalcitrant xenobiotics. In this study, it was found that oxalic acid, veratryl alcohol and 2,6-Dimehoxyphenol could stimulate the synthesis of MnP in the white-rot fungus <i>Irpex lacteus</i> CD2. A novel manganese peroxidase named as CD2-MnP was purified and characterized from this fungus. CD2-MnP had a strong capability for tolerating different metal ions such as Ca²⁺, Cd²⁺, Co²⁺, Mg²⁺, Ni²⁺ and Zn²⁺ as well as organic solvents such as methanol, ethanol, DMSO, ethylene glycol, isopropyl alcohol, butanediol and glycerin. The different types of dyes including the azo dye (Remazol Brilliant Violet 5R, Direct Red 5B), anthraquinone dye (Remazol Brilliant Blue R), indigo dye (Indigo Carmine) and triphenylmethane dye (Methyl Green) as well as simulated textile wastewater could be efficiently decolorized by CD2-MnP. CD2-MnP also had a strong ability of decolorizing different dyes with the coexistence of metal ions and organic solvents. In summary, CD2-MnP from <i>Irpex lacteus</i> CD2 could effectively degrade a broad range of synthetic dyes and exhibit a great potential for environmental biotechnology.</p></div

Effect of metal ions on the stability of purified CD2-MnP from <i>Irpex lacteus</i> CD2.

<p>The MnP activity of control without adding any metal compound was set as 100%.</p><p>Effect of metal ions on the stability of purified CD2-MnP from <i>Irpex lacteus</i> CD2.</p

Effect of metal ions and organic solvents on the activity of purified CD2-MnP.

<p><b>A</b>: The effect of different metal ions on MnP activity. The MnP activity of the control without adding any metal compound was set as 100%. <b>B</b>: The effect of different organic solvents on MnP activity. The MnP activity of the control without adding any organic solvent was set as 100%.</p

Decolorization of different types of dyes by the purified CD2-MnP with the coexistence of metal ions.

<p>The reaction mixture in a total volume 1 ml contained (final concentration): malonate buffer (20 mM, pH 4.5), Mn²⁺ (1.6 mM), H₂O₂ (0.08 mM), purified CD2-MnP (0.25 U/ml), dye (50 mg/L) and Ca²⁺, Co²⁺, Mg²⁺, Zn²⁺ (4 mM). <b>CK (MnP+H₂O₂)</b> was the control without addition of any metal compound except Mn²⁺. <b>H₂O₂ (no MnP)</b> was the negative control without addition of purified CD2-MnP. (<b>A</b>): Decolorization of RBV5R; (<b>B</b>): Decolorization of DR5B; (<b>C</b>): Decolorization of RBBR; (<b>D</b>): Decolorization of IC; (<b>E</b>): Decolorization of MG. <b>RBV5R</b>: Remazol Brilliant Violet 5R, <b>DR5B</b>: Direct Red 5B, <b>RBBR</b>: Remazol Brilliant Blue R, <b>IC</b>: Indigo Carmine, <b>MG</b>: Methyl Green. The negative control (H₂O₂ was added into the decolorization mixture in the absence of purified CD2-MnP) showed no significant decolorization of different dyes.</p

Effect of organic solvents on the stability of purified CD2-MnP from <i>Irpex lacteus</i> CD2.

<p>The MnP activity of control without adding any organic solvent was set as 100%.</p><p>Effect of organic solvents on the stability of purified CD2-MnP from <i>Irpex lacteus</i> CD2.</p