テクスチャー画像および絵画に対する複雑さの知覚モデルの構築

This thesis devotes to building a relationship between human visual complexity perception and objective image features. Visual complexity of images is a worth-well investigation. It has a wide range of applications from computer science (emotion semantic image retrieval, digital watermarking and image analysis, etc.) to arts (the design of product surface, wallpapers, painting appreciation and selection, etc.). Various methods for computing image complexity have been developed depending on different applications, such as information theory, fractal dimension, quad tree method, etc. However, these measures are not sufficiently related to human visual perception of complexity. Intuitively, visual complexity is influenced by various factors perceived by humans from the image, not directly related to simple objective measures like distribution of spatial frequencies.……広島大学(Hiroshima University)博士(工学)Engineeringdoctora

Exonuclease III protection assay with FRET probe for detecting DNA-binding proteins

We describe a new method for the assay of sequence-specific DNA-binding proteins in this paper. In this method, the sensitive fluorescence resonance energy transfer (FRET) technology is combined with the common DNA footprinting assay in order to develop a simple, rapid and high-throughput approach for quantitatively detecting the sequence-specific DNA-binding proteins. We named this method as exonuclease III (ExoIII) protection assay with FRET probe. The FRET probe used in this assay was a duplex DNA which was designed to contain one FRET pair in the center and two flanking protein-binding sites. During protein detection, if a target protein exists, it will bind to the two protein-binding sites of the FRET probe and thus protect the FRET pair from ExoIII digestion, resulting in high FRET. However, if the target protein does not exist, the FRET pair on the naked FRET probe will be degraded by ExoIII, resulting in low FRET. Three kinds of recombinant transcription factors including NF-κB, SP1 and p50, and the target protein of NF-κB in HeLa cell nuclear extracts, were successfully detected by the assay. This assay can be extensively used in biomedical research targeted at DNA-binding proteins

An acousto-optic modulator based bi-frequency interferometer for quantum technology

Acousto-optic modulators (AOMs) have been widely used in quantum optical technology, but the non-ideal diffraction efficiency limits its application in a quantum system. Here we demonstrate a bi-frequency interferometer using AOMs as both the beam-splitter and the beam-combiner. The intensity of the input light can be as low as the single photon level, and the interferometer can work in a chopped phase locking mode. The modulation for the phase locking scheme is realized on the beam-splitting AOM driven by specially designed radio frequency signal, which avoids using extra optical modulators and makes the quantum efficiency of the system as high as $(95\pm1)\%$. By optimizing the factors that affect the mode matching, the visibility of the beating signal for the interferometer is $(99.5\pm0.2)\%$. This near prefect visibility allows the interferometer to be applied in high efficiency quantum technical schemes while leaving the diffraction efficiencies of each AOM for about $50\%$. This greatly reduced the demand for the driving of AOMs.Comment: 6 pages, 4 figure