Additional file 4: of Cecal MicroRNAome response to Salmonella enterica serovar Enteritidis infection in White Leghorn Layer

Enriched GO BP terms in each group. (XLS 100 kb

Additional file 2: of Cecal MicroRNAome response to Salmonella enterica serovar Enteritidis infection in White Leghorn Layer

Density distribution of miRNAs across chromosomes. Note: Densities are shown as number of miRNAs per megabase of DNA. (TIFF 256 kb

Phenolic Ligand–Metal Charge Transfer Induced Copper Nanozyme with Reactive Oxygen Species-Scavenging Ability for Chronic Wound Healing

Chronic wounds frequently arise as a complication in diabetic patients, and their management remains a significant clinical hurdle due to their nonhealing nature featured by heightened oxidative stress and impaired healing cells at the wound site. Herein, we present a 2D copper antioxidant nanozyme induced by phenolic ligand–metal charge transfer (LMCT) to eliminate reactive oxygen species (ROS) and facilitate the healing of chronic diabetic wounds. We found that polyphenol ligands coordinated on the Cu3(PO4)2 nanosheets led to a strong charge transfer at the interface and regulated the valence states of Cu. The obtained Cu nanozyme exhibited efficient scavenging ability toward different oxidative species and protected human cells from oxidative damage. The nanozyme enhanced the healing of diabetic wounds by promoting re-epithelialization, collagen deposition, angiogenesis, and immunoregulation. This work demonstrates the LMCT-induced ROS scavenging ability on a nanointerface, providing an alternative strategy of constructing metal-based nanozymes for the treatment of diabetic wounds as well as other diseases

Pattern ERG responses in the Normal, Edaravone and Vehicle groups.

<p>(A) Average contrast implicit time of P50 response in the three groups. (B) Average contrast implicit time of N95 response in the three groups. (C) Average contrast amplitude of P50 response in the three groups. (D) Average contrast amplitude of N95 response in the three groups. The nonparametric Kruskal-Wallis test followed by the Mann-Whitney test was used for multiple-group comparison. Values are the means ± SD of three independent experiments.*P<0.05, ***P<0.001 vs. Normal, ^#P<0.05 vs. Edaravone.</p

Eeffects of edaravone on retinal ERK1/2 activation, BDNF expression and cleavage of caspase-3 in diabetic retinopathy.

<p>(A) Data are expressed as percentage change in phosphorylated ERK1/2 (p-ERK1/2) over total ERK1/2 (t-ERK1/2) (B) The histogram represents the mean band intensity of BDNF adjusted to the intensity of β-actin in the same sample. (C) The ratio of active caspase-3 (17 kDa) and pro-caspase-3 (37 kDa) was calculated in control and diabetic mice maintained with and without Edaravone treatment. Measurements were made in duplicate in six to eight mice in each group. Western blots are representative of three different experiments. Results are expressed as mean±SD. Values obtained from Normal group are considered as 100%. *P<0.05, ***P<0.001 vs. Normal, ^#P<0.05 vs. Edaravone.</p

Eeffects of edaravone on RGC death with TUNEL staining.

<p>Quantification of TUNEL (+) cells showed significantly decreased TUNEL (+) cells/mm² with treatment of edaravone, compared with the streptozotocin-treated group. Data are expressed as the mean±SD. *P<0.05, ***P<0.001 vs. Normal, ^#P<0.05 vs. Edaravone. Original magnification: ×10; scale bar, 100 µm.</p

Quasi-Two-Dimensional SiC and SiC₂: Interaction of Silicon and Carbon at Atomic Thin Lattice Plane

The band gap of graphene is nearly zero, and thus novel two-dimensional (2D) semiconductor and band gap engineering of graphene is highly desired for advanced optoelectronic applications. Herein, we have experimentally produced quasi-two-dimensional (quasi-2D) SiC by reaction between graphene and a silicon source, which was designed and supported by Born–Oppenheimer molecular dynamics simulations. The lateral length of the as-synthesized quasi-2D SiC is mainly in the range of 0.3–5 μm while the thickness is commonly below 10 nm. Quasi-2D SiC₂ is also found as a byproduct, which is stable over 3 months in air atmosphere. The exciton binding energy of quasi-2D multilayers SiC can reach 0.23 eV while the band gap is around 3.72 eV. Additionally, in situ transmission electron microscopy has firmly proven that quasi-2D SiC can be synthesized through the reaction between graphene and silicon quantum dots. The first production of quasi-2D SiC and SiC₂ makes the band gap engineering in the graphene lattice plane possible

Effects of Astragaloside IV on pattern ERG outcomes.

<p>Data are expressed as the mean ± SD. A. Pattern ERG amplitudes in all groups. NC group received significantly decreased amplitudes compared with LC group (NC vs LC, p = 0.037, * p<0.05). Mice in Astragaloside IV treatment groups have increased amplitudes compared with NC group (AL, AH vs NC, p = 0.005, 0.037 respectively. ## p<0.01; # p<0.05). Amplitudes have no significance between AL and AH group. B. Pattern ERG latency in all groups. Astragaloside IV treatment changed the latency, but latency levels did not reveal a statistically significant difference.</p

Pattern ERGs derived from each eye in response to pattern reversal of horizontal gratings in a population of 48 mice aged 28 weeks.

<p>Individual waveforms of each eye are shown superimposed in each group. Mean amplitude in each group is regarded as a consult. A: LC, B: NC, C: AL, D: AH.</p

The effect of astragaloside IV on the apoptosis of retina cells.

<p>Apoptotic cells were marked with green fluorescence, the nuclei of cells are stained by blue fluorescence (DAPI). A: LC, B: NC, C: AL, D: AH. GCL, retinal ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Original magnification: ×20; scale bar, 100 mm. Triangles: Apoptotic cells.</p