Frequency-dependent AVO attribute: theory and example

Fluid-saturated rocks generally have seismic velocities that depend upon frequency. Exploring this property may help us discriminate different fluids from seismic data. In this paper, we introduce a scheme to calculate a frequency-dependent AVO attribute in order to estimate seismic dispersion from pre-stack data, and apply it to North Sea data. The scheme essentially combines the two-term approximation of Smith and Gidlow (1987) with the method of spectral decomposition based on the Wigner-Ville distribution, which is used to achieve high resolution. The result suggests the potential of this method for detection of seismic dispersion due to fluid saturation

Regulation of Nuclear Hormone Receptors by Corepressors and Coactivators: a Dissertation

Nuclear hormone receptors (NHR) constitute a superfamily of ligand inducible transcriptional activators that enable an organism to regulate development and homeostasis through switching on or off target genes in response to stimuli reflecting changes in environment as well as endocrine. NHRs include classical steroid hormone receptors (GR, AR, ER and MR) and retinoid, thyroid hormone receptors. One long-term goal of our lab is to understand the molecular mechanisms through which the transcriptional activity of NHRs is regulated. Extensive studies in the past few years have revealed that in addition to the dependence on ligand availability, the transcriptional activity of NHRs is also regulated by two types of proteins: co activators and corepressors. In the absence of ligand, many NHRs, including TR and RAR can actively repress target gene transcription with the help of corepressors, proteins that physically interact with both NHRs and histone deacetylases (HDACs). Functional interactions between NHRs and corepressors therefore lead to tightly compact and transcriptionally non-permissive chromatin structures after the removal of obstructive acetyl groups from histone tails by HDACs. On the other hand, ligand binding stabilizes NHRs in a conformation that favors interaction with proteins other than corepressors; many of these proteins are able to potentiate the transcriptional activity of NHRs through various mechanisms, such as histone acetylation, chromatin remodeling and recruitment of basal transcription machinery and are collectively termed coactivators. Two highly related corepressors, SMRT (silencing mediator of retinoid and thyroid hormone receptors) and N-CoR (nuclear receptor corepressor), have been cloned. This research in corepressor SMRT started by a systematic study of its subcellular localization. We found that SMRT predominantly forms a specific nuclear punctuate structure that does not appear to overlap with any other well-known subnuclear domains/speckles. Although our searching for specific sequence signals that may determine the specific speckle localization of SMRT did not yield conclusive results, we discovered the colocalization of unliganded RAR and certain HDACs, including HDAC1, 3,4 and 5, in the SMRT nuclear speckles. Moreover, SMRT is likely to be the organizer of such speckles since it appears to be able to recruit other proteins into these speckles. The presence of HDAC1 in the SMRT speckles suggests a direct association between these two proteins, which has not been detected by previous biochemical analyses. Interestingly, HDAC1 point mutants that are completely defective in deacetylase activity failed to locate to SMRT nuclear speckles, while another partially active mutant maintained the colocalization. These discoveries may indicate SMRT nuclear speckles as novel nuclear domains involved in transcriptional repression. More physiologically relevant support for this hypothesis arises from study of HDAC4 and 5. HDAC4 and 5 are potent inhibitors of transcriptional activator MEF2C. Nuclear presence of HDAC4/5 can block the activation of MEF2C, which is required during muscle differentiation. Normally, HDAC4 is predominantly located in cytoplasm. However, we found that in the presence of SMRT overexpression, HDAC4 was found mostly in SMRT nuclear speckles. This accumulation enhanced HDAC4 mediated inhibition on MEF2C transcriptional activity in a transient transfection assay. SMRT overexpression also resulted in accumulation of HDAC5 in the SMRT nuclear speckles compared to the nuclear diffuse distribution in the absence of SMRT. Again, this accumulation of HDAC5 in nuclear speckles correlated with enhanced inhibition of MEF2C. Taken together, our study suggested that instead of being merely a corepressor for NHRs, SMRT might function as an organizer of a nuclear repression domain, which may be involved in a broad array of cellular processes. In contrast to the limited number of corepressors, numerous co activators have been identified; the SRC (or p160) family is relatively well studied. This family includes three highly related members, SRC-1, TIF2/GRIP1, RAC3/AIB1/ACTR/p/CIP. Similar domain structures are shared among these factors, with the most highly conserved region, the bHLH-PAS domain found within the N terminal ~400 amino acid residues. This study of RAC3 aims to identify the function of the highly conserved N terminal bHLH-PAS domain by isolating interacting proteins through yeast two-hybrid screening. One candidate gene isolated encodes the C terminal fragment of the human homologue of the yeast protein MMS19. Functional studies of this small fragment revealed that it specifically interacted with human estrogen receptors (ERs) and inhibited ligand induced transcriptional activity of ERs in the transient transfection assay. Then we cloned the full-length human MMS19 cDNA and characterized the hMMS19 as a weak coactivator for estrogen receptors in the transient transfection assay. Furthermore, when tested on separate AF-1 or AF-2 of ERs, hMMS19 specifically enhanced AF-1 but had no effect on AF-2. These results identified hMMS19 as a specific coactivator for ER AF-1

The Supreme People\u27s Court\u27s Annual Report on Intellectual Property Cases (2016) (China)

The Supreme People’s Court of China began publishing its Annual Report on Intellectual Property Cases in 2008. The Annual Report summarizes intellectual property cases, such as patent, trademark, copyright, trade secrets, and unfair competition cases. This 2016 Annual Report examines 27 cases and includes general guidelines for legal application. It reflects the Supreme People’s Court’s thoughts and approaches for ruling on new, difficult, and complex IP and competition cases

Interpretation of phase reversals in seismic reflections from attenuating targets

Phase reversals are commonly observed during exploration seismic amplitude-versus-offset analysis, and usually modelled with the Zoeppritz equations. In practice, when multiple fluids are present we often deal with reflections from interfaces having a contrast in both elastic and anelastic properties. In this paper, we present a new phenomenological model for phase reversals in cases in which velocity dispersion and attenuation are present. We demonstrate strong qualitative differences in behaviour between the elastic and anelastic cases, influencing both the amplitude and phase of the reflection coefficient. Analysis of seismic data showing a phase reversal from a gas reservoir in the Vienna basin shows a striking agreement with the modelling. We conclude that the influence of fluid-induced dispersion on reflection data is a significant and measurable phenomenon in cases of practical interest, that recognition of the phenomena may be used as a novel fluid indicator and that frequency-dependent rock physics analysis may be an important tool for both industrial geophysics and seismic monitoring of CO2 storage

Estimating seismic dispersion from prestack data using frequency-dependent AVO analysis

Recent laboratory measurement studies have suggested a growing consensus that fluid saturated rocks can have frequency-dependent properties within the seismic bandwidth. It is appealing to try to use these properties for the discrimination of fluid saturation from seismic data. In this paper, we develop a frequency-dependent AVO (FAVO) attribute to measure magnitude of dispersion from pre-stack data. The scheme essentially extends the Smith and Gidlow (1987)’s two-term AVO approximation to be frequency-dependent, and then linearize the frequency-dependent approximation with Taylor series expansion. The magnitude of dispersion can be estimated with least-square inversion. A high-resolution spectral decomposition method is of vital importance during the implementation of the FAVO attribute calculation. We discuss the resolution of three typical spectral decomposition techniques: the short term Fourier transform (STFT), continuous wavelet transform (CWT) and Wigner-Vill Distribution (WVD) based methods. The smoothed pseudo Wigner-Ville Distribution (SPWVD) method, which uses smooth windows in time and frequency domain to suppress cross-terms, provides higher resolution than that of STFT and CWT. We use SPWVD in the FAVO attribute to calculate the frequency-dependent spectral amplitudes from pre-stack data. We test our attribute on forward models with different time scales and crack densities to understand wave-scatter induced dispersion at the interface between an elastic shale and a dispersive sandstone. The FAVO attribute can determine the maximum magnitude of P-wave dispersion for dispersive partial gas saturation case; higher crack density gives rise to stronger magnitude of P-wave dispersion. Finally, the FAVO attribute was applied to real seismic data from the North Sea. The result suggests the potential of this method for detection of seismic dispersion due to fluid saturation

Simulated identification on dynamic characteristics of large heavy-load bearing

It’s difficult to test repeatedly for large heavy-load bearings (LHLBs) with full-scale and real load due to complexity and costliness, so simulated identification on dynamic characteristics of 1750 MW nuclear generator bearing with diameter 800 mm and specific pressure 3.3 MPa is provided in this paper. The identification model of bearing dynamic characteristic is established, the calculating method of positive and negative dynamic problems is provided, and effects of signal disturbances on identification precision are analyzed. The results show that the LHLBs’ permitted displacement disturbance should not be over 5 μm and the permitted ratio of dynamic load and static load is about 1 %-2 %, which is different from common knowledge of 15 %-20 % for small light-load bearings. If identification error of the main stiffness and main damping coefficients is less than 5 %, the amplitude of periodical disturbance of the dynamic load and displacement signals should be less than 5 %. If identification error of the main damping coefficients is less than 10 %, the phase of these two signals should be less than 1°. The roundness error and rotation error of the large shaft should be eliminated