Sequential FISH and GISH karyotypes of M8003 (a), Austrian rye (b), N9116H (c) and N9116M (d).

<p>(a, c, d) 4',6-diamidino-2-phenylindole (DAPI), blue fluorescence; rye genomic DNA and Oligo-pTa535, red fluorescence; Oligo-pSc119.2, green fluorescence. (b) Oligo-pSc119.2, red fluorescence. Alterations of wheat chromosomes were indicated in white box.</p

Morphologic traits of hexaploid triticales N9116H/M and their parents.

<p>Morphologic traits of hexaploid triticales N9116H/M and their parents.</p

Morphologic traits of M8003, Austrian rye, N9116H and N9116M.

<p>a Plant of M8003, Austrian rye, N9116M and N9116H; b Spikes of M8003, Austrian rye, N9116M and N9116H; c Spikelets and kernels of M8003, Austrian rye, N9116H and N9116M; d Resistance of M8003, Austrian rye, N9116H and N9116M for powdery mildew and stripe rust. 1–4 in figures represent M8003, Austrian rye, N9116M and N9116H, respectively.</p

Breeding scheme showing the development of N9116H and N9116M.

<p>Breeding scheme showing the development of N9116H and N9116M.</p

Storage protein variations in hexaploid titicales N9116H and N9116M.

<p>1–6 represent Xiaoyan No.6, Chinese Spring, M8003, Austrian rye, N9116H and N9116M, respectively. Black arrow, position of inherited HMW-GS subunit of Austrian rye; red arrow, novel HMW-GS subunit emerged in N9116H and N9116M; red triangle, novel secalins emerged in N9116H; red box, HMW-GS variations in N9116H and N9116M.</p