Additional file 2 of Network pharmacology -based study on the mechanism of traditional Chinese medicine in the treatment of glioblastoma multiforme

Additional file 2: Supplementary Table 2. Active ingredients and target genes of YQQYJDF

Additional file 1 of Network pharmacology -based study on the mechanism of traditional Chinese medicine in the treatment of glioblastoma multiforme

Additional file 1: Supplementary Table 1. Genes in WGCNA color modules related to the prognosis of GBM patients

Additional file 3 of Network pharmacology -based study on the mechanism of traditional Chinese medicine in the treatment of glioblastoma multiforme

Additional file 3: Supplementary Table 3. YQQYJDF targets in the treatment of GBM

Additional file 3: of Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease

Figure S3. Altered surface expression density of monocytes in COPD patients. Classical (a-c) and non-classical monocytes (panels d-f) were stained for CCR2 (a, d), CCR5 (b, e), and CD14 (c, f) expression. The degree of expression is reported as the mean fluorescence intensity (MFI). * = p < 0.05 and ** = p < 0.01 relative to the normal. (PDF 13 kb

Additional file 1: of Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease

Figure S1. Flow cytometry gating strategy to identify and characterize monocyte subpopulations. PMBCs were stained as described in the Methods section and at least 250,000 events per sample were collected. Singlets (red rectangle, panel a) were gated using the forward side-scatter area (FSC-A) vs height (FSC-H). From the singlets gate, monocytes (red oval, panel b) were gated using the FSC-A vs side-scatter area (SSC-A). The monocytes were further gated using CD14 vs CD16 and are indicated by the red boxes (panel c). The classical monocytes are CD14 + CD16-; the intermediate monocytes are CD14 + CD16+; and the non-classical monocytes are CD14DIMCD16+. From the classical gate, cells stained for CCR2, CCR5, CD163, CD206, and IL-13Ra1 are shown (panels i-m), and from the non-classical gate, the staining for CCR2, CCR5, CD163, CD206, and IL-13Ra1 are shown in panels d-h. The red histograms indicate the isotype control for each marker. The black histograms indicated the expression of each marker. (PDF 311 kb

Additional file 4: of Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease

Figure S4. Increased CX3CR1 expression density in CD206 + CCR5+ non-classical monocytes in severe COPD patients. CD206 + CCR5+ co-expressing cells were stained for CX3CR1, and the mean fluorescence intensity (MFI) for each patient population was determined. Results represent the mean MFI ± SEM of all subjects in each subject group. * = p < 0.05. (PDF 4 kb

Additional file 2: of Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease

Figure S2. Analysis of CCR2 and CCR5 expression by classical and non-classical monocytes. Classical (a, c, e) and non-classical (b, d, f) monocytes were stained for CCR2 and CCR5 expression. The data are presented for the percentage of CCR2-positive (a, b), CCR5-positive (c, d), and CCR2- and CCR5-double positive (e, f) monocytes. The data are presented as the percentage of total classical or non-classical monocytes for each group. (PDF 13 kb

Additional file 5: of Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease

Figure S5. Reduced numbers of CD206 + CCR5+ monocytes in severe COPD. CD206 + CCR5+ classical (a) and CD206 + CCR5+ non-classical (b) monocytes data were expressed as the number of cells per μl. * = p < 0.05; ** = p < 0.01; and *** = p < 0.001 relative to the normal. (PDF 11 kb

Endogenous PS externalization in apoptotic EBV-transformed human B lymphocytes.

<p>EBV-transformed normal (A–D) or Tangier (E–H) B lymphocytes were untreated (A&B, E&F) or treated with camptothecin (C&D, G&H) to induce apoptosis, stained with fluorescent annexin V, and examined by flow cytometry. Left panel, forward (FS) vs side (SS) light scatter plots, with cells of normal size and shape in the R2 gate and shrunken cells in the R1 gate. Right panel, fluorescence profile of cells in the R2 gate.</p

Internalization (translocation) of NBD-labeled phospholipids by normal human fibroblasts and fibroblasts from Tangier individuals.

<p>The outer leaflet of the plasma membrane of normal (circles) or Tangier (squares) fibroblasts was labeled with NBD-PC (open symbols) or NBD-PS (filled symbols). At various times samples were removed into BSA to extract outer leaflet probe and remaining inner leaflet probe was measured by flow cytometry and expressed as percent transported. Measurements were made at room temperature. (A) Viable fibroblasts, (B) spontaneously apoptotic fibroblasts. Dashed and dotted lines in B have been redrawn from A for transport by normal fibroblasts.</p