2 research outputs found
Fluorescence Probe Based on an Amino-Functionalized Fluorescent Magnetic Nanocomposite for Detection of Folic Acid in Serum
A new
fluorescence probe constructed with a multifunctional nanocomposite,
Fe<sub>3</sub>O<sub>4</sub>–ZnS:Mn<sup>2+</sup>/SiO<sub>2</sub>–NH<sub>2</sub>, was successfully synthesized and then used
to detect folic acid in real serum samples. The nanocomposite was
characterized by fluorescence spectroscopy, transmission electron
microscopy, Fourier transform infrared spectroscopy, X-ray powder
diffraction, and physical property measurement system. With the addition
of analyte, the Fe<sub>3</sub>O<sub>4</sub>–ZnS:Mn<sup>2+</sup>/SiO<sub>2</sub>–NH<sub>2</sub> composite and folic acid formed
a new complex because cross-linking of the amino and carboxyl groups
participated in the condensation reaction. Then, the energy of quantum
dots was transferred to the complex and led to quenching of the fluorescence.
Moreover, the fluorescence intensity decreased significantly as the
concentration of folic acid increased, and the fluorescence quenching
ratio <i>F</i><sub>0</sub>/<i>F</i> was related
to the folic acid concentration in the range from 0.1 to 5 μg
mL<sup>–1</sup>. This method was used for detecting folic acid
in real serum samples and gave recoveries in the range of 89.0%–96.0%,
with relative standard deviations of 1.2%–3.9%. The detection
limit was 9.6 ng mL<sup>–1</sup> (S/N = 3). These satisfactory
and simple results showed the great potential of this fluorescence
probe in the field of pharmaceutical analysis
DataSheet1_Oxidative Stress in Parkinson's Disease: A Systematic Review and Meta-Analysis.docx
<p>Oxidative stress has been suggested to play a key role in Parkinson's disease, but inconsistent results were found in clinical studies. This study sought to quantitatively summarize the blood and cerebrospinal fluid (CSF) oxidative stress marker data in PD patients. We performed a systematic search of PubMed and Web of Science, and studies were included if they provided data on peripheral blood and CSF oxidative stress marker concentrations in PD patients and healthy control (HC) subjects. Data were extracted by three independent investigators from 80 included studies encompassing 7,212 PD patients and 6,037 HC subjects. Of the 22 oxidative stress markers analyzed, random effects meta-analysis showed that blood concentrations of 8-OhdG, MDA, nitrite, and ferritin were increased in patients with PD compared with HC subjects. In contrast, we showed that blood levels of catalase, uric acid, glutathione, and total-cholesterol were significantly down-regulated in patients with PD when compared with controls. There were no significant differences between PD patients and HC subjects for blood, Mn, Cu, Zn, Fe, SOD, albumin, glutathione peroxidase, vitamin E, ceruloplasmin, triglycerides, lactoferrin, transferrin, LDL-cholesterol, and HDL-cholesterol. Due to the limited number of CSF studies with small sample size, this meta-analysis only showed non-significant association between CSF 8-OhdG and PD. The findings of our meta-analysis demonstrated higher blood concentrations of 8-OhdG, MDA, nitrite and ferritin, and lower blood concentrations of catalase, uric acid, glutathione and total-cholesterol in PD patients, strengthening the clinical evidence that PD is accompanied by increased oxidative stress.</p