1 research outputs found
DNA Assembly of Plasmonic Nanostructures Enables <i>In Vivo</i> SERS-Based MicroRNA Detection and Tumor Photoacoustic Imaging
Controllable self-assembly of the DNA-linked gold nanoparticle
(AuNP) architecture for in vivo biomedical applications
remains a key challenge. Here, we describe the use of the programmed
DNA tetrahedral structure to control the assembly of three different
types of AuNPs (ā¼20, 10, and 5 nm) by organizing them into
defined positioning and arrangement. A DNA-assembled ācoreāsatelliteā
architecture is built by DNA sequencing where satellite AuNPs (10
and 5 nm) surround a central core AuNP (20 nm). The density and arrangement
of the AuNP satellites around the core AuNP were controlled by tuning
the size and amount of the DNA tetrahedron functionalized on the core
AuNPs, resulting in strong electromagnetic field enhancement derived
from hybridized plasmonic coupling effects. By conjugating with the
Raman molecule, strong surface-enhanced Raman scattering photoacoustic
imaging signals could be generated, which were able to image microRNA-21
and tumor tissues in vivo. These results provided
an efficient strategy to build precision plasmonic superstructures
in plasmonic-based bioanalysis and imaging